Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4817 and GFF4818 are separated by 87 nucleotides GFF4818 and GFF4819 are separated by 75 nucleotides GFF4819 and GFF4820 overlap by 1 nucleotides
GFF4817 - Secreted protein, at 116,985 to 118,067
GFF4817
GFF4818 - Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8), at 118,155 to 118,889
GFF4818
GFF4819 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 118,965 to 120,677
GFF4819
GFF4820 - Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2), at 120,677 to 122,476
GFF4820
Position (kb)
118
119
120
121 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 117.999 kb on + strand at 118.131 kb on + strand at 118.131 kb on + strand at 118.139 kb on + strand at 119.721 kb on - strand, within GFF4819 at 119.892 kb on - strand, within GFF4819 at 119.892 kb on - strand, within GFF4819 at 120.167 kb on - strand, within GFF4819 at 120.194 kb on - strand, within GFF4819 at 120.206 kb on + strand, within GFF4819 at 120.219 kb on - strand, within GFF4819 at 120.505 kb on + strand, within GFF4819 at 120.599 kb on - strand at 120.638 kb on + strand at 120.707 kb on + strand at 120.722 kb on + strand at 120.828 kb on + strand at 120.829 kb on - strand at 120.829 kb on - strand at 120.832 kb on + strand at 120.897 kb on + strand, within GFF4820 at 120.964 kb on - strand, within GFF4820 at 121.033 kb on + strand, within GFF4820 at 121.270 kb on + strand, within GFF4820 at 121.315 kb on - strand, within GFF4820 at 121.416 kb on + strand, within GFF4820 at 121.445 kb on + strand, within GFF4820
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 117,999 + -0.1 118,131 + +0.4 118,131 + +0.0 118,139 + +0.6 119,721 - GFF4819 0.44 -0.7 119,892 - GFF4819 0.54 -0.4 119,892 - GFF4819 0.54 -0.3 120,167 - GFF4819 0.70 +0.1 120,194 - GFF4819 0.72 -0.0 120,206 + GFF4819 0.72 -0.2 120,219 - GFF4819 0.73 -1.5 120,505 + GFF4819 0.90 -0.9 120,599 - -3.8 120,638 + -0.9 120,707 + -2.2 120,722 + -0.7 120,828 + -1.7 120,829 - +0.4 120,829 - -0.4 120,832 + -3.7 120,897 + GFF4820 0.12 -2.3 120,964 - GFF4820 0.16 -0.2 121,033 + GFF4820 0.20 -3.8 121,270 + GFF4820 0.33 -2.1 121,315 - GFF4820 0.35 +0.5 121,416 + GFF4820 0.41 -1.7 121,445 + GFF4820 0.43 -0.9
Or see this region's nucleotide sequence