Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4797 and GFF4798 are separated by 95 nucleotides GFF4798 and GFF4799 overlap by 14 nucleotides GFF4799 and GFF4800 are separated by 9 nucleotides
GFF4797 - Putative cytochrome oxidase subunit, at 101,759 to 102,037
GFF4797
GFF4798 - Adenylylsulfate kinase (EC 2.7.1.25), at 102,133 to 102,738
GFF4798
GFF4799 - Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4), at 102,725 to 104,164
GFF4799
GFF4800 - Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4), at 104,174 to 105,082
GFF4800
Position (kb) 102
103
104
105 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 101.732 kb on - strand at 101.765 kb on + strand at 101.768 kb on + strand at 101.775 kb on - strand at 101.776 kb on + strand at 101.776 kb on + strand at 101.810 kb on + strand, within GFF4797 at 101.810 kb on + strand, within GFF4797 at 101.915 kb on - strand, within GFF4797 at 101.917 kb on - strand, within GFF4797 at 101.917 kb on - strand, within GFF4797 at 102.074 kb on - strand at 102.076 kb on + strand at 102.077 kb on - strand at 102.085 kb on + strand at 102.086 kb on - strand at 102.115 kb on + strand at 102.115 kb on + strand at 102.116 kb on - strand at 102.116 kb on - strand at 102.328 kb on - strand, within GFF4798 at 102.347 kb on + strand, within GFF4798 at 102.560 kb on + strand, within GFF4798 at 102.561 kb on - strand, within GFF4798 at 102.583 kb on + strand, within GFF4798 at 102.584 kb on - strand, within GFF4798 at 102.822 kb on + strand at 102.939 kb on + strand, within GFF4799 at 102.996 kb on - strand, within GFF4799 at 103.232 kb on + strand, within GFF4799 at 103.411 kb on - strand, within GFF4799 at 103.443 kb on - strand, within GFF4799 at 103.454 kb on + strand, within GFF4799 at 103.455 kb on - strand, within GFF4799 at 103.460 kb on + strand, within GFF4799 at 103.706 kb on - strand, within GFF4799 at 103.711 kb on + strand, within GFF4799 at 103.796 kb on - strand, within GFF4799 at 103.804 kb on + strand, within GFF4799 at 103.804 kb on + strand, within GFF4799 at 103.805 kb on - strand, within GFF4799 at 103.837 kb on + strand, within GFF4799 at 103.882 kb on + strand, within GFF4799 at 103.886 kb on - strand, within GFF4799 at 103.919 kb on + strand, within GFF4799 at 103.976 kb on - strand, within GFF4799 at 104.153 kb on + strand at 104.154 kb on - strand at 104.176 kb on - strand at 104.182 kb on - strand at 104.494 kb on - strand, within GFF4800 at 104.572 kb on - strand, within GFF4800 at 104.727 kb on - strand, within GFF4800 at 104.812 kb on + strand, within GFF4800 at 104.813 kb on - strand, within GFF4800 at 104.816 kb on + strand, within GFF4800 at 104.865 kb on + strand, within GFF4800 at 104.865 kb on + strand, within GFF4800 at 104.865 kb on + strand, within GFF4800 at 104.904 kb on - strand, within GFF4800 at 104.982 kb on + strand, within GFF4800 at 104.983 kb on - strand, within GFF4800 at 105.025 kb on + strand at 105.083 kb on - strand at 105.125 kb on + strand at 105.136 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 101,732 - -1.9 101,765 + -0.4 101,768 + -1.6 101,775 - -0.9 101,776 + -0.8 101,776 + -0.1 101,810 + GFF4797 0.18 -0.4 101,810 + GFF4797 0.18 -0.8 101,915 - GFF4797 0.56 -1.2 101,917 - GFF4797 0.57 -2.6 101,917 - GFF4797 0.57 +0.2 102,074 - -0.5 102,076 + -1.0 102,077 - -0.6 102,085 + -0.5 102,086 - +1.0 102,115 + -0.0 102,115 + -1.6 102,116 - +1.2 102,116 - +1.2 102,328 - GFF4798 0.32 -5.5 102,347 + GFF4798 0.35 -2.2 102,560 + GFF4798 0.70 -2.7 102,561 - GFF4798 0.71 -1.9 102,583 + GFF4798 0.74 -2.1 102,584 - GFF4798 0.74 -2.0 102,822 + -1.1 102,939 + GFF4799 0.15 +0.1 102,996 - GFF4799 0.19 +0.2 103,232 + GFF4799 0.35 +0.2 103,411 - GFF4799 0.48 +0.9 103,443 - GFF4799 0.50 -2.8 103,454 + GFF4799 0.51 -0.1 103,455 - GFF4799 0.51 +0.4 103,460 + GFF4799 0.51 -0.2 103,706 - GFF4799 0.68 +0.4 103,711 + GFF4799 0.68 +0.8 103,796 - GFF4799 0.74 +0.4 103,804 + GFF4799 0.75 -1.7 103,804 + GFF4799 0.75 +0.4 103,805 - GFF4799 0.75 -0.5 103,837 + GFF4799 0.77 +0.0 103,882 + GFF4799 0.80 +0.2 103,886 - GFF4799 0.81 -0.2 103,919 + GFF4799 0.83 -0.1 103,976 - GFF4799 0.87 -0.1 104,153 + +0.6 104,154 - -0.2 104,176 - +0.7 104,182 - +1.1 104,494 - GFF4800 0.35 -0.6 104,572 - GFF4800 0.44 +0.3 104,727 - GFF4800 0.61 +0.3 104,812 + GFF4800 0.70 -0.7 104,813 - GFF4800 0.70 -0.1 104,816 + GFF4800 0.71 +0.1 104,865 + GFF4800 0.76 +0.0 104,865 + GFF4800 0.76 -0.1 104,865 + GFF4800 0.76 +0.7 104,904 - GFF4800 0.80 -1.1 104,982 + GFF4800 0.89 -0.1 104,983 - GFF4800 0.89 -0.6 105,025 + +0.1 105,083 - +1.1 105,125 + -0.9 105,136 - +0.8
Or see this region's nucleotide sequence