Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4703 and GFF4704 are separated by 185 nucleotides GFF4704 and GFF4705 overlap by 4 nucleotides
GFF4703 - Anaerobic nitric oxide reductase transcription regulator NorR, at 13,145 to 14,665
GFF4703
GFF4704 - Anaerobic nitric oxide reductase flavorubredoxin, at 14,851 to 16,290
GFF4704
GFF4705 - Nitric oxide reductase FlRd-NAD(+) reductase (EC 1.18.1.-), at 16,287 to 17,420
GFF4705
Position (kb) 14
15
16
17 Strain fitness (log2 ratio) -1
0
1 at 13.886 kb on + strand, within GFF4703 at 13.886 kb on + strand, within GFF4703 at 13.886 kb on + strand, within GFF4703 at 14.191 kb on + strand, within GFF4703 at 14.192 kb on - strand, within GFF4703 at 14.461 kb on + strand, within GFF4703 at 14.693 kb on + strand at 14.954 kb on - strand at 15.070 kb on + strand, within GFF4704 at 15.071 kb on - strand, within GFF4704 at 15.148 kb on - strand, within GFF4704 at 15.151 kb on + strand, within GFF4704 at 15.177 kb on + strand, within GFF4704 at 15.313 kb on + strand, within GFF4704 at 15.313 kb on + strand, within GFF4704 at 15.314 kb on - strand, within GFF4704 at 15.314 kb on - strand, within GFF4704 at 15.365 kb on - strand, within GFF4704 at 15.433 kb on + strand, within GFF4704 at 15.433 kb on + strand, within GFF4704 at 15.574 kb on + strand, within GFF4704 at 15.574 kb on + strand, within GFF4704 at 15.574 kb on + strand, within GFF4704 at 15.574 kb on + strand, within GFF4704 at 15.575 kb on - strand, within GFF4704 at 15.575 kb on - strand, within GFF4704 at 15.596 kb on - strand, within GFF4704 at 15.609 kb on + strand, within GFF4704 at 15.643 kb on - strand, within GFF4704 at 15.643 kb on - strand, within GFF4704 at 15.643 kb on - strand, within GFF4704 at 15.789 kb on + strand, within GFF4704 at 15.790 kb on - strand, within GFF4704 at 16.095 kb on - strand, within GFF4704 at 16.139 kb on + strand, within GFF4704 at 16.139 kb on + strand, within GFF4704 at 16.139 kb on + strand, within GFF4704 at 16.153 kb on + strand at 16.153 kb on + strand at 16.248 kb on - strand at 16.307 kb on + strand at 16.308 kb on - strand at 16.434 kb on - strand, within GFF4705 at 16.521 kb on - strand, within GFF4705 at 16.572 kb on - strand, within GFF4705 at 16.649 kb on - strand, within GFF4705 at 16.664 kb on + strand, within GFF4705 at 16.754 kb on - strand, within GFF4705 at 16.852 kb on + strand, within GFF4705 at 16.853 kb on - strand, within GFF4705 at 16.949 kb on - strand, within GFF4705 at 16.959 kb on - strand, within GFF4705 at 16.962 kb on - strand, within GFF4705 at 16.996 kb on + strand, within GFF4705 at 17.060 kb on + strand, within GFF4705 at 17.061 kb on - strand, within GFF4705 at 17.064 kb on + strand, within GFF4705 at 17.069 kb on - strand, within GFF4705 at 17.091 kb on + strand, within GFF4705 at 17.209 kb on + strand, within GFF4705
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 13,886 + GFF4703 0.49 +0.3 13,886 + GFF4703 0.49 -0.7 13,886 + GFF4703 0.49 -0.0 14,191 + GFF4703 0.69 +0.9 14,192 - GFF4703 0.69 -1.0 14,461 + GFF4703 0.87 +1.7 14,693 + -0.4 14,954 - +0.4 15,070 + GFF4704 0.15 -1.0 15,071 - GFF4704 0.15 -0.1 15,148 - GFF4704 0.21 +0.5 15,151 + GFF4704 0.21 +0.6 15,177 + GFF4704 0.23 +0.8 15,313 + GFF4704 0.32 -0.0 15,313 + GFF4704 0.32 -0.3 15,314 - GFF4704 0.32 +0.3 15,314 - GFF4704 0.32 +0.7 15,365 - GFF4704 0.36 -0.0 15,433 + GFF4704 0.40 -0.6 15,433 + GFF4704 0.40 +0.1 15,574 + GFF4704 0.50 -1.6 15,574 + GFF4704 0.50 +0.2 15,574 + GFF4704 0.50 +0.6 15,574 + GFF4704 0.50 -0.2 15,575 - GFF4704 0.50 -0.1 15,575 - GFF4704 0.50 +1.2 15,596 - GFF4704 0.52 +0.4 15,609 + GFF4704 0.53 +1.1 15,643 - GFF4704 0.55 +0.0 15,643 - GFF4704 0.55 -0.3 15,643 - GFF4704 0.55 +0.7 15,789 + GFF4704 0.65 -0.0 15,790 - GFF4704 0.65 +0.1 16,095 - GFF4704 0.86 -0.8 16,139 + GFF4704 0.89 -0.3 16,139 + GFF4704 0.89 +1.5 16,139 + GFF4704 0.89 -0.4 16,153 + +0.1 16,153 + -0.5 16,248 - +0.4 16,307 + +1.2 16,308 - -1.0 16,434 - GFF4705 0.13 -1.4 16,521 - GFF4705 0.21 +1.2 16,572 - GFF4705 0.25 -0.2 16,649 - GFF4705 0.32 +0.2 16,664 + GFF4705 0.33 +1.2 16,754 - GFF4705 0.41 +0.6 16,852 + GFF4705 0.50 -0.1 16,853 - GFF4705 0.50 +0.4 16,949 - GFF4705 0.58 +0.5 16,959 - GFF4705 0.59 +0.7 16,962 - GFF4705 0.60 +0.7 16,996 + GFF4705 0.63 +0.8 17,060 + GFF4705 0.68 +0.4 17,061 - GFF4705 0.68 +0.1 17,064 + GFF4705 0.69 +0.4 17,069 - GFF4705 0.69 +0.1 17,091 + GFF4705 0.71 -0.2 17,209 + GFF4705 0.81 -0.0
Or see this region's nucleotide sequence