Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4380 and GFF4381 are separated by 141 nucleotides GFF4381 and GFF4382 are separated by 124 nucleotides GFF4382 and GFF4383 are separated by 41 nucleotides
GFF4380 - Membrane fusion protein of RND family multidrug efflux pump, at 162,633 to 163,826
GFF4380
GFF4381 - Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family, at 163,968 to 164,621
GFF4381
GFF4382 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 164,746 to 168,102
GFF4382
GFF4383 - Mobile element protein, at 168,144 to 169,079
GFF4383
Position (kb) 164
165
166
167
168
169 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 163.898 kb on - strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.920 kb on + strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.921 kb on - strand at 163.928 kb on + strand at 163.929 kb on - strand at 163.934 kb on + strand at 163.935 kb on - strand at 163.967 kb on + strand at 164.113 kb on - strand, within GFF4381 at 164.141 kb on - strand, within GFF4381 at 164.231 kb on - strand, within GFF4381 at 164.291 kb on - strand, within GFF4381 at 164.304 kb on - strand, within GFF4381 at 164.411 kb on + strand, within GFF4381 at 164.571 kb on + strand at 164.572 kb on - strand at 164.572 kb on - strand at 164.626 kb on - strand at 164.746 kb on - strand at 164.904 kb on + strand at 164.931 kb on + strand at 164.963 kb on + strand at 165.088 kb on - strand, within GFF4382 at 165.199 kb on + strand, within GFF4382 at 165.202 kb on - strand, within GFF4382 at 165.256 kb on + strand, within GFF4382 at 165.257 kb on - strand, within GFF4382 at 165.258 kb on + strand, within GFF4382 at 165.259 kb on - strand, within GFF4382 at 165.275 kb on + strand, within GFF4382 at 165.347 kb on + strand, within GFF4382 at 165.350 kb on + strand, within GFF4382 at 165.414 kb on + strand, within GFF4382 at 165.414 kb on + strand, within GFF4382 at 165.452 kb on - strand, within GFF4382 at 165.780 kb on + strand, within GFF4382 at 165.788 kb on - strand, within GFF4382 at 165.831 kb on + strand, within GFF4382 at 165.831 kb on + strand, within GFF4382 at 166.005 kb on - strand, within GFF4382 at 166.021 kb on - strand, within GFF4382 at 166.021 kb on - strand, within GFF4382 at 166.150 kb on - strand, within GFF4382 at 166.327 kb on + strand, within GFF4382 at 166.372 kb on - strand, within GFF4382 at 166.503 kb on + strand, within GFF4382 at 166.507 kb on - strand, within GFF4382 at 166.632 kb on - strand, within GFF4382 at 166.751 kb on + strand, within GFF4382 at 166.751 kb on + strand, within GFF4382 at 166.885 kb on + strand, within GFF4382 at 166.885 kb on + strand, within GFF4382 at 166.938 kb on + strand, within GFF4382 at 166.977 kb on - strand, within GFF4382 at 166.982 kb on + strand, within GFF4382 at 167.115 kb on + strand, within GFF4382 at 167.116 kb on - strand, within GFF4382 at 167.129 kb on + strand, within GFF4382 at 167.187 kb on - strand, within GFF4382 at 167.187 kb on - strand, within GFF4382 at 167.208 kb on + strand, within GFF4382 at 167.208 kb on + strand, within GFF4382 at 167.209 kb on - strand, within GFF4382 at 167.222 kb on - strand, within GFF4382 at 167.279 kb on + strand, within GFF4382 at 167.279 kb on + strand, within GFF4382 at 167.280 kb on - strand, within GFF4382 at 167.410 kb on + strand, within GFF4382 at 167.410 kb on + strand, within GFF4382 at 167.411 kb on - strand, within GFF4382 at 167.469 kb on + strand, within GFF4382 at 167.650 kb on - strand, within GFF4382 at 167.908 kb on + strand at 167.988 kb on + strand at 167.989 kb on - strand at 168.091 kb on - strand at 168.100 kb on + strand at 168.366 kb on - strand, within GFF4383 at 168.438 kb on - strand, within GFF4383 at 168.439 kb on + strand, within GFF4383 at 168.634 kb on + strand, within GFF4383 at 168.636 kb on + strand, within GFF4383 at 168.636 kb on + strand, within GFF4383 at 168.668 kb on + strand, within GFF4383 at 168.669 kb on - strand, within GFF4383 at 168.669 kb on - strand, within GFF4383 at 168.669 kb on - strand, within GFF4383 at 168.669 kb on - strand, within GFF4383 at 168.672 kb on - strand, within GFF4383 at 168.680 kb on + strand, within GFF4383 at 168.681 kb on - strand, within GFF4383 at 168.716 kb on - strand, within GFF4383 at 168.887 kb on - strand, within GFF4383
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 163,898 - -1.1 163,920 + +0.3 163,920 + +0.8 163,920 + +0.6 163,920 + +0.5 163,920 + +0.4 163,920 + +0.2 163,921 - -0.4 163,921 - -0.3 163,921 - -0.3 163,921 - +0.2 163,921 - +0.4 163,921 - -0.5 163,928 + +0.7 163,929 - +1.0 163,934 + -0.3 163,935 - -0.6 163,967 + +0.3 164,113 - GFF4381 0.22 -0.8 164,141 - GFF4381 0.26 -1.9 164,231 - GFF4381 0.40 +0.5 164,291 - GFF4381 0.49 -0.9 164,304 - GFF4381 0.51 -0.5 164,411 + GFF4381 0.68 +0.1 164,571 + -0.4 164,572 - -0.0 164,572 - +0.4 164,626 - -2.0 164,746 - +0.4 164,904 + -0.5 164,931 + -0.0 164,963 + -0.1 165,088 - GFF4382 0.10 +1.7 165,199 + GFF4382 0.13 -1.0 165,202 - GFF4382 0.14 +0.7 165,256 + GFF4382 0.15 +0.3 165,257 - GFF4382 0.15 +0.2 165,258 + GFF4382 0.15 +0.8 165,259 - GFF4382 0.15 -0.1 165,275 + GFF4382 0.16 +1.6 165,347 + GFF4382 0.18 -0.2 165,350 + GFF4382 0.18 +0.2 165,414 + GFF4382 0.20 +0.5 165,414 + GFF4382 0.20 -0.0 165,452 - GFF4382 0.21 +0.4 165,780 + GFF4382 0.31 -0.1 165,788 - GFF4382 0.31 +0.6 165,831 + GFF4382 0.32 +0.4 165,831 + GFF4382 0.32 -0.5 166,005 - GFF4382 0.38 -0.1 166,021 - GFF4382 0.38 +0.2 166,021 - GFF4382 0.38 +0.4 166,150 - GFF4382 0.42 -0.1 166,327 + GFF4382 0.47 -0.8 166,372 - GFF4382 0.48 +1.3 166,503 + GFF4382 0.52 +0.8 166,507 - GFF4382 0.52 +0.9 166,632 - GFF4382 0.56 +0.6 166,751 + GFF4382 0.60 -3.1 166,751 + GFF4382 0.60 -1.1 166,885 + GFF4382 0.64 -1.7 166,885 + GFF4382 0.64 -1.9 166,938 + GFF4382 0.65 +0.5 166,977 - GFF4382 0.66 +1.2 166,982 + GFF4382 0.67 +0.8 167,115 + GFF4382 0.71 +0.9 167,116 - GFF4382 0.71 +0.2 167,129 + GFF4382 0.71 +0.1 167,187 - GFF4382 0.73 -0.8 167,187 - GFF4382 0.73 +1.4 167,208 + GFF4382 0.73 +0.1 167,208 + GFF4382 0.73 -0.1 167,209 - GFF4382 0.73 +0.1 167,222 - GFF4382 0.74 +1.2 167,279 + GFF4382 0.75 +0.0 167,279 + GFF4382 0.75 -1.0 167,280 - GFF4382 0.75 +0.5 167,410 + GFF4382 0.79 -2.0 167,410 + GFF4382 0.79 -0.1 167,411 - GFF4382 0.79 +0.3 167,469 + GFF4382 0.81 -0.0 167,650 - GFF4382 0.87 +1.4 167,908 + +2.0 167,988 + -0.2 167,989 - +2.9 168,091 - -0.2 168,100 + -0.3 168,366 - GFF4383 0.24 -0.2 168,438 - GFF4383 0.31 +0.4 168,439 + GFF4383 0.32 -0.6 168,634 + GFF4383 0.52 +0.4 168,636 + GFF4383 0.53 +0.1 168,636 + GFF4383 0.53 +0.1 168,668 + GFF4383 0.56 -0.7 168,669 - GFF4383 0.56 +1.1 168,669 - GFF4383 0.56 +0.2 168,669 - GFF4383 0.56 -0.5 168,669 - GFF4383 0.56 -1.2 168,672 - GFF4383 0.56 +0.0 168,680 + GFF4383 0.57 +0.6 168,681 - GFF4383 0.57 -0.1 168,716 - GFF4383 0.61 +0.9 168,887 - GFF4383 0.79 +0.5
Or see this region's nucleotide sequence