Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4318

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4315 and GFF4316 are separated by 74 nucleotidesGFF4316 and GFF4317 are separated by 44 nucleotidesGFF4317 and GFF4318 are separated by 3 nucleotidesGFF4318 and GFF4319 are separated by 88 nucleotidesGFF4319 and GFF4320 are separated by 20 nucleotides GFF4315 - Hypothetical lipoprotein yajI, at 98,912 to 99,409 GFF4315 GFF4316 - hypothetical protein, at 99,484 to 99,630 GFF4316 GFF4317 - Ribonucleotide reductase transcriptional regulator NrdR, at 99,675 to 100,052 GFF4317 GFF4318 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 100,056 to 101,159 GFF4318 GFF4319 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 101,248 to 101,718 GFF4319 GFF4320 - Transcription termination protein NusB, at 101,739 to 102,158 GFF4320 Position (kb) 100 101 102Strain fitness (log2 ratio) -1 0 1 2at 99.098 kb on + strand, within GFF4315at 99.150 kb on - strand, within GFF4315at 99.182 kb on - strand, within GFF4315at 99.204 kb on - strand, within GFF4315at 99.482 kb on + strandat 99.569 kb on + strand, within GFF4316at 99.570 kb on - strand, within GFF4316at 99.628 kb on + strandat 99.705 kb on - strandat 99.717 kb on - strand, within GFF4317at 99.795 kb on - strand, within GFF4317at 99.795 kb on - strand, within GFF4317at 99.795 kb on - strand, within GFF4317at 99.875 kb on + strand, within GFF4317at 99.875 kb on + strand, within GFF4317at 99.974 kb on + strand, within GFF4317at 99.974 kb on + strand, within GFF4317at 100.050 kb on + strandat 101.144 kb on + strandat 101.144 kb on + strandat 101.144 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
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99,098 + GFF4315 0.37 -0.1
99,150 - GFF4315 0.48 +0.5
99,182 - GFF4315 0.54 +0.7
99,204 - GFF4315 0.59 +0.0
99,482 + -1.6
99,569 + GFF4316 0.58 +0.5
99,570 - GFF4316 0.59 +1.3
99,628 + +1.8
99,705 - +1.0
99,717 - GFF4317 0.11 +0.7
99,795 - GFF4317 0.32 +0.7
99,795 - GFF4317 0.32 +0.4
99,795 - GFF4317 0.32 +1.4
99,875 + GFF4317 0.53 +1.9
99,875 + GFF4317 0.53 +1.3
99,974 + GFF4317 0.79 +2.6
99,974 + GFF4317 0.79 +2.0
100,050 + -1.0
101,144 + -0.3
101,144 + -0.3
101,144 + -0.1

Or see this region's nucleotide sequence