Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4315 and GFF4316 are separated by 74 nucleotides GFF4316 and GFF4317 are separated by 44 nucleotides GFF4317 and GFF4318 are separated by 3 nucleotides GFF4318 and GFF4319 are separated by 88 nucleotides GFF4319 and GFF4320 are separated by 20 nucleotides
GFF4315 - Hypothetical lipoprotein yajI, at 98,912 to 99,409
GFF4315
GFF4316 - hypothetical protein, at 99,484 to 99,630
GFF4316
GFF4317 - Ribonucleotide reductase transcriptional regulator NrdR, at 99,675 to 100,052
GFF4317
GFF4318 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 100,056 to 101,159
GFF4318
GFF4319 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 101,248 to 101,718
GFF4319
GFF4320 - Transcription termination protein NusB, at 101,739 to 102,158
GFF4320
Position (kb)
100
101
102 Strain fitness (log2 ratio)
-1
0
1
2 at 99.098 kb on + strand, within GFF4315 at 99.150 kb on - strand, within GFF4315 at 99.182 kb on - strand, within GFF4315 at 99.204 kb on - strand, within GFF4315 at 99.482 kb on + strand at 99.569 kb on + strand, within GFF4316 at 99.570 kb on - strand, within GFF4316 at 99.628 kb on + strand at 99.705 kb on - strand at 99.717 kb on - strand, within GFF4317 at 99.795 kb on - strand, within GFF4317 at 99.795 kb on - strand, within GFF4317 at 99.795 kb on - strand, within GFF4317 at 99.875 kb on + strand, within GFF4317 at 99.875 kb on + strand, within GFF4317 at 99.974 kb on + strand, within GFF4317 at 99.974 kb on + strand, within GFF4317 at 100.050 kb on + strand at 101.144 kb on + strand at 101.144 kb on + strand at 101.144 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 99,098 + GFF4315 0.37 -0.1 99,150 - GFF4315 0.48 +0.5 99,182 - GFF4315 0.54 +0.7 99,204 - GFF4315 0.59 +0.0 99,482 + -1.6 99,569 + GFF4316 0.58 +0.5 99,570 - GFF4316 0.59 +1.3 99,628 + +1.8 99,705 - +1.0 99,717 - GFF4317 0.11 +0.7 99,795 - GFF4317 0.32 +0.7 99,795 - GFF4317 0.32 +0.4 99,795 - GFF4317 0.32 +1.4 99,875 + GFF4317 0.53 +1.9 99,875 + GFF4317 0.53 +1.3 99,974 + GFF4317 0.79 +2.6 99,974 + GFF4317 0.79 +2.0 100,050 + -1.0 101,144 + -0.3 101,144 + -0.3 101,144 + -0.1
Or see this region's nucleotide sequence