Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4193 and GFF4194 are separated by 41 nucleotides GFF4194 and GFF4195 are separated by 9 nucleotides GFF4195 and GFF4196 are separated by 40 nucleotides GFF4196 and GFF4197 are separated by 92 nucleotides
GFF4193 - FIG01200173: hypothetical protein, at 8,063 to 8,653
GFF4193
GFF4194 - Endonuclease V (EC 3.1.21.7), at 8,695 to 9,366
GFF4194
GFF4195 - Uroporphyrinogen III decarboxylase (EC 4.1.1.37), at 9,376 to 10,440
GFF4195
GFF4196 - NADH pyrophosphatase (EC 3.6.1.22), at 10,481 to 11,254
GFF4196
GFF4197 - Regulator of sigma D, at 11,347 to 11,835
GFF4197
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 8.392 kb on - strand, within GFF4193 at 8.400 kb on + strand, within GFF4193 at 8.400 kb on + strand, within GFF4193 at 8.401 kb on - strand, within GFF4193 at 8.511 kb on + strand, within GFF4193 at 8.556 kb on + strand, within GFF4193 at 8.557 kb on - strand, within GFF4193 at 8.557 kb on - strand, within GFF4193 at 8.565 kb on + strand, within GFF4193 at 8.591 kb on - strand, within GFF4193 at 8.597 kb on - strand at 8.648 kb on + strand at 9.149 kb on + strand, within GFF4194 at 9.149 kb on + strand, within GFF4194 at 9.149 kb on + strand, within GFF4194 at 9.150 kb on - strand, within GFF4194 at 9.150 kb on - strand, within GFF4194 at 9.150 kb on - strand, within GFF4194 at 9.182 kb on + strand, within GFF4194 at 9.183 kb on - strand, within GFF4194 at 9.183 kb on - strand, within GFF4194 at 9.218 kb on - strand, within GFF4194 at 9.369 kb on + strand at 9.377 kb on + strand at 9.378 kb on - strand at 10.474 kb on + strand at 10.474 kb on + strand at 10.475 kb on - strand at 10.475 kb on - strand at 10.554 kb on - strand at 10.557 kb on - strand at 10.557 kb on - strand at 10.632 kb on + strand, within GFF4196 at 10.632 kb on + strand, within GFF4196 at 10.692 kb on + strand, within GFF4196 at 10.755 kb on - strand, within GFF4196 at 10.793 kb on - strand, within GFF4196 at 10.887 kb on + strand, within GFF4196 at 10.942 kb on + strand, within GFF4196 at 10.943 kb on - strand, within GFF4196 at 10.957 kb on - strand, within GFF4196 at 10.997 kb on + strand, within GFF4196 at 11.063 kb on - strand, within GFF4196 at 11.082 kb on - strand, within GFF4196 at 11.082 kb on - strand, within GFF4196 at 11.172 kb on - strand, within GFF4196 at 11.243 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.255 kb on - strand at 11.255 kb on - strand at 11.255 kb on - strand at 11.325 kb on + strand at 11.329 kb on + strand at 11.330 kb on - strand at 11.330 kb on - strand at 11.352 kb on - strand at 11.420 kb on + strand, within GFF4197 at 11.424 kb on + strand, within GFF4197 at 11.424 kb on + strand, within GFF4197 at 11.425 kb on - strand, within GFF4197
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 8,392 - GFF4193 0.56 -0.2 8,400 + GFF4193 0.57 +1.3 8,400 + GFF4193 0.57 -1.4 8,401 - GFF4193 0.57 +0.2 8,511 + GFF4193 0.76 +2.5 8,556 + GFF4193 0.83 +1.2 8,557 - GFF4193 0.84 +0.7 8,557 - GFF4193 0.84 +0.6 8,565 + GFF4193 0.85 +0.5 8,591 - GFF4193 0.89 +1.3 8,597 - +1.7 8,648 + +0.5 9,149 + GFF4194 0.68 -0.5 9,149 + GFF4194 0.68 +0.2 9,149 + GFF4194 0.68 -0.3 9,150 - GFF4194 0.68 +0.0 9,150 - GFF4194 0.68 +1.2 9,150 - GFF4194 0.68 -0.0 9,182 + GFF4194 0.72 -0.4 9,183 - GFF4194 0.73 +0.0 9,183 - GFF4194 0.73 -0.3 9,218 - GFF4194 0.78 -0.2 9,369 + +0.3 9,377 + +0.2 9,378 - -0.1 10,474 + -2.2 10,474 + +0.4 10,475 - -0.2 10,475 - -0.0 10,554 - -0.7 10,557 - -0.7 10,557 - +1.2 10,632 + GFF4196 0.20 +0.3 10,632 + GFF4196 0.20 -1.3 10,692 + GFF4196 0.27 -1.0 10,755 - GFF4196 0.35 +0.6 10,793 - GFF4196 0.40 +0.1 10,887 + GFF4196 0.52 -0.0 10,942 + GFF4196 0.60 -0.1 10,943 - GFF4196 0.60 +1.0 10,957 - GFF4196 0.61 -0.5 10,997 + GFF4196 0.67 -0.0 11,063 - GFF4196 0.75 +0.6 11,082 - GFF4196 0.78 -0.2 11,082 - GFF4196 0.78 -0.8 11,172 - GFF4196 0.89 +0.3 11,243 + -0.1 11,254 + -0.7 11,254 + +0.0 11,254 + +0.7 11,254 + -0.1 11,255 - +0.0 11,255 - +0.6 11,255 - +0.1 11,325 + -0.5 11,329 + +0.3 11,330 - -0.7 11,330 - -0.4 11,352 - +1.3 11,420 + GFF4197 0.15 +0.4 11,424 + GFF4197 0.16 +0.4 11,424 + GFF4197 0.16 -0.3 11,425 - GFF4197 0.16 +0.2
Or see this region's nucleotide sequence