Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF406 and GFF407 overlap by 20 nucleotides GFF407 and GFF408 overlap by 33 nucleotides GFF408 and GFF409 are separated by 63 nucleotides GFF409 and GFF410 overlap by 4 nucleotides GFF410 and GFF411 overlap by 4 nucleotides
GFF406 - Transcriptional regulator, LacI family, at 121,891 to 122,946
GFF406
GFF407 - hypothetical protein, at 122,927 to 123,046
GFF407
GFF408 - 'tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207)' transl_table=11, at 123,014 to 123,487
GFF408
GFF409 - L-lactate dehydrogenase (EC 1.1.2.3), at 123,551 to 124,741
GFF409
GFF410 - Lactate-responsive regulator LldR in Enterobacteria, GntR family, at 124,738 to 125,514
GFF410
GFF411 - L-lactate permease, at 125,511 to 127,166
GFF411
Position (kb)
123
124
125 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 122.553 kb on - strand, within GFF406 at 122.553 kb on - strand, within GFF406 at 122.556 kb on + strand, within GFF406 at 122.556 kb on + strand, within GFF406 at 122.557 kb on - strand, within GFF406 at 122.811 kb on + strand, within GFF406 at 122.814 kb on + strand, within GFF406 at 123.009 kb on - strand, within GFF407 at 123.010 kb on + strand, within GFF407 at 123.010 kb on + strand, within GFF407 at 123.010 kb on + strand, within GFF407 at 123.011 kb on - strand, within GFF407 at 123.064 kb on - strand, within GFF408 at 123.142 kb on + strand, within GFF408 at 123.216 kb on + strand, within GFF408 at 123.216 kb on + strand, within GFF408 at 123.217 kb on - strand, within GFF408 at 123.301 kb on - strand, within GFF408 at 123.377 kb on - strand, within GFF408 at 123.473 kb on + strand at 123.474 kb on - strand at 123.748 kb on - strand, within GFF409 at 124.063 kb on + strand, within GFF409 at 124.113 kb on + strand, within GFF409 at 124.130 kb on + strand, within GFF409 at 124.174 kb on + strand, within GFF409 at 124.197 kb on + strand, within GFF409 at 124.491 kb on + strand, within GFF409 at 124.492 kb on - strand, within GFF409 at 124.492 kb on - strand, within GFF409 at 124.643 kb on - strand at 124.644 kb on + strand at 124.746 kb on + strand at 124.767 kb on + strand at 124.767 kb on + strand at 124.768 kb on - strand at 124.869 kb on + strand, within GFF410 at 124.958 kb on + strand, within GFF410 at 124.958 kb on + strand, within GFF410 at 124.959 kb on - strand, within GFF410 at 124.962 kb on + strand, within GFF410 at 124.962 kb on + strand, within GFF410 at 124.963 kb on - strand, within GFF410 at 124.963 kb on - strand, within GFF410 at 125.387 kb on - strand, within GFF410 at 125.549 kb on + strand at 125.567 kb on + strand at 125.567 kb on + strand at 125.568 kb on - strand at 125.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 122,553 - GFF406 0.63 +0.6 122,553 - GFF406 0.63 -0.0 122,556 + GFF406 0.63 +0.1 122,556 + GFF406 0.63 -0.5 122,557 - GFF406 0.63 -0.2 122,811 + GFF406 0.87 -0.5 122,814 + GFF406 0.87 +1.3 123,009 - GFF407 0.68 +0.2 123,010 + GFF407 0.69 -0.9 123,010 + GFF407 0.69 +0.2 123,010 + GFF407 0.69 -0.7 123,011 - GFF407 0.70 -0.5 123,064 - GFF408 0.11 +1.9 123,142 + GFF408 0.27 -0.5 123,216 + GFF408 0.43 +0.6 123,216 + GFF408 0.43 +1.4 123,217 - GFF408 0.43 -0.1 123,301 - GFF408 0.61 +0.6 123,377 - GFF408 0.77 +0.4 123,473 + -0.1 123,474 - +0.3 123,748 - GFF409 0.17 -0.3 124,063 + GFF409 0.43 +1.1 124,113 + GFF409 0.47 +0.2 124,130 + GFF409 0.49 -0.3 124,174 + GFF409 0.52 -0.5 124,197 + GFF409 0.54 -1.9 124,491 + GFF409 0.79 +0.7 124,492 - GFF409 0.79 -0.3 124,492 - GFF409 0.79 +0.1 124,643 - -0.2 124,644 + +0.1 124,746 + -0.2 124,767 + -0.4 124,767 + +0.6 124,768 - +0.5 124,869 + GFF410 0.17 +0.1 124,958 + GFF410 0.28 +0.4 124,958 + GFF410 0.28 +0.1 124,959 - GFF410 0.28 -0.3 124,962 + GFF410 0.29 +1.0 124,962 + GFF410 0.29 +1.0 124,963 - GFF410 0.29 +0.2 124,963 - GFF410 0.29 +0.4 125,387 - GFF410 0.84 +0.8 125,549 + -0.1 125,567 + -0.8 125,567 + -0.4 125,568 - +0.3 125,568 - +0.7
Or see this region's nucleotide sequence