Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4066 and GFF4067 are separated by 277 nucleotides GFF4067 and GFF4068 are separated by 33 nucleotides GFF4068 and GFF4069 overlap by 1 nucleotides GFF4069 and GFF4070 are separated by 26 nucleotides
GFF4066 - Putative inner membrane protein, at 27,830 to 28,603
GFF4066
GFF4067 - putative Shiga-like toxin A subunit, at 28,881 to 29,237
GFF4067
GFF4068 - IcmF-related protein, at 29,271 to 33,140
GFF4068
GFF4069 - FIG074102: hypothetical protein, at 33,140 to 33,928
GFF4069
GFF4070 - Probable secreted protein, at 33,955 to 34,341
GFF4070
Position (kb) 29
30
31
32
33
34 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 28.402 kb on + strand, within GFF4066 at 28.421 kb on + strand, within GFF4066 at 28.509 kb on - strand, within GFF4066 at 28.654 kb on - strand at 29.475 kb on - strand at 29.642 kb on + strand at 29.968 kb on + strand, within GFF4068 at 29.969 kb on - strand, within GFF4068 at 29.969 kb on - strand, within GFF4068 at 29.987 kb on + strand, within GFF4068 at 30.025 kb on - strand, within GFF4068 at 30.483 kb on + strand, within GFF4068 at 30.492 kb on - strand, within GFF4068 at 30.512 kb on + strand, within GFF4068 at 30.772 kb on - strand, within GFF4068 at 31.002 kb on + strand, within GFF4068 at 31.119 kb on + strand, within GFF4068 at 31.212 kb on + strand, within GFF4068 at 31.212 kb on + strand, within GFF4068 at 31.248 kb on - strand, within GFF4068 at 31.248 kb on - strand, within GFF4068 at 31.276 kb on - strand, within GFF4068 at 31.310 kb on + strand, within GFF4068 at 31.315 kb on - strand, within GFF4068 at 31.512 kb on - strand, within GFF4068 at 31.520 kb on + strand, within GFF4068 at 31.884 kb on - strand, within GFF4068 at 31.893 kb on - strand, within GFF4068 at 32.092 kb on + strand, within GFF4068 at 32.117 kb on + strand, within GFF4068 at 32.262 kb on + strand, within GFF4068 at 32.263 kb on - strand, within GFF4068 at 32.263 kb on - strand, within GFF4068 at 32.265 kb on + strand, within GFF4068 at 32.266 kb on - strand, within GFF4068 at 32.266 kb on - strand, within GFF4068 at 32.289 kb on - strand, within GFF4068 at 32.765 kb on + strand at 32.765 kb on + strand at 32.765 kb on + strand at 32.863 kb on + strand at 33.084 kb on - strand at 33.203 kb on + strand at 33.367 kb on + strand, within GFF4069 at 33.368 kb on - strand, within GFF4069 at 33.512 kb on + strand, within GFF4069 at 33.602 kb on - strand, within GFF4069 at 33.765 kb on + strand, within GFF4069 at 33.876 kb on - strand at 33.910 kb on + strand at 33.954 kb on + strand at 33.986 kb on + strand at 34.011 kb on - strand, within GFF4070 at 34.080 kb on + strand, within GFF4070 at 34.081 kb on - strand, within GFF4070
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 28,402 + GFF4066 0.74 -1.0 28,421 + GFF4066 0.76 -0.1 28,509 - GFF4066 0.88 +0.0 28,654 - -0.2 29,475 - -0.0 29,642 + +0.6 29,968 + GFF4068 0.18 -0.5 29,969 - GFF4068 0.18 +0.6 29,969 - GFF4068 0.18 +0.2 29,987 + GFF4068 0.19 +0.2 30,025 - GFF4068 0.19 -2.3 30,483 + GFF4068 0.31 +0.1 30,492 - GFF4068 0.32 -0.1 30,512 + GFF4068 0.32 -0.7 30,772 - GFF4068 0.39 +0.8 31,002 + GFF4068 0.45 -0.5 31,119 + GFF4068 0.48 -0.8 31,212 + GFF4068 0.50 +0.8 31,212 + GFF4068 0.50 -0.2 31,248 - GFF4068 0.51 -0.3 31,248 - GFF4068 0.51 +0.8 31,276 - GFF4068 0.52 -0.4 31,310 + GFF4068 0.53 -0.3 31,315 - GFF4068 0.53 -0.1 31,512 - GFF4068 0.58 +0.8 31,520 + GFF4068 0.58 -1.6 31,884 - GFF4068 0.68 +0.9 31,893 - GFF4068 0.68 +0.5 32,092 + GFF4068 0.73 -0.6 32,117 + GFF4068 0.74 -0.2 32,262 + GFF4068 0.77 -0.2 32,263 - GFF4068 0.77 -1.6 32,263 - GFF4068 0.77 +0.4 32,265 + GFF4068 0.77 -2.9 32,266 - GFF4068 0.77 -0.0 32,266 - GFF4068 0.77 -0.1 32,289 - GFF4068 0.78 -0.3 32,765 + +0.6 32,765 + -0.3 32,765 + -0.2 32,863 + -0.1 33,084 - -0.5 33,203 + -1.1 33,367 + GFF4069 0.29 -0.7 33,368 - GFF4069 0.29 -0.2 33,512 + GFF4069 0.47 -0.1 33,602 - GFF4069 0.59 -0.4 33,765 + GFF4069 0.79 -1.4 33,876 - -0.1 33,910 + +0.3 33,954 + +0.3 33,986 + -0.1 34,011 - GFF4070 0.14 -0.2 34,080 + GFF4070 0.32 -0.2 34,081 - GFF4070 0.33 +0.4
Or see this region's nucleotide sequence