Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3875 and GFF3876 are separated by 2 nucleotides GFF3876 and GFF3877 are separated by 2 nucleotides GFF3877 and GFF3878 overlap by 4 nucleotides
GFF3875 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 151,128 to 152,249
GFF3875
GFF3876 - GDP-L-fucose synthetase (EC 1.1.1.271), at 152,252 to 153,217
GFF3876
GFF3877 - GDP-mannose mannosyl hydrolase (EC 3.6.1.-), at 153,220 to 153,693
GFF3877
GFF3878 - Colanic acid biosysnthesis glycosyl transferase WcaI, at 153,690 to 154,913
GFF3878
Position (kb)
152
153
154 Strain fitness (log2 ratio)
-1
0
1
2 at 151.265 kb on + strand, within GFF3875 at 151.377 kb on + strand, within GFF3875 at 151.377 kb on + strand, within GFF3875 at 151.378 kb on - strand, within GFF3875 at 151.378 kb on - strand, within GFF3875 at 151.656 kb on - strand, within GFF3875 at 151.824 kb on + strand, within GFF3875 at 151.849 kb on + strand, within GFF3875 at 152.257 kb on + strand at 152.578 kb on + strand, within GFF3876 at 152.579 kb on - strand, within GFF3876 at 152.658 kb on - strand, within GFF3876 at 152.699 kb on + strand, within GFF3876 at 152.700 kb on - strand, within GFF3876 at 152.914 kb on + strand, within GFF3876 at 153.176 kb on + strand at 153.177 kb on - strand at 153.177 kb on - strand at 153.227 kb on + strand at 153.485 kb on - strand, within GFF3877 at 153.485 kb on - strand, within GFF3877 at 153.706 kb on - strand at 153.713 kb on + strand at 153.713 kb on + strand at 153.713 kb on + strand at 153.906 kb on + strand, within GFF3878 at 153.906 kb on + strand, within GFF3878 at 154.091 kb on - strand, within GFF3878
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 151,265 + GFF3875 0.12 +1.4 151,377 + GFF3875 0.22 +0.2 151,377 + GFF3875 0.22 +1.4 151,378 - GFF3875 0.22 -0.3 151,378 - GFF3875 0.22 +0.4 151,656 - GFF3875 0.47 +0.1 151,824 + GFF3875 0.62 +0.2 151,849 + GFF3875 0.64 +1.8 152,257 + +0.5 152,578 + GFF3876 0.34 -0.9 152,579 - GFF3876 0.34 -0.3 152,658 - GFF3876 0.42 +0.4 152,699 + GFF3876 0.46 -1.1 152,700 - GFF3876 0.46 -0.5 152,914 + GFF3876 0.69 +0.4 153,176 + +1.5 153,177 - -0.2 153,177 - +0.4 153,227 + +0.7 153,485 - GFF3877 0.56 +0.5 153,485 - GFF3877 0.56 +0.4 153,706 - +0.3 153,713 + +1.8 153,713 + +1.8 153,713 + +0.6 153,906 + GFF3878 0.18 +1.5 153,906 + GFF3878 0.18 +1.8 154,091 - GFF3878 0.33 -0.5
Or see this region's nucleotide sequence