Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3706 and GFF3707 are separated by 108 nucleotides GFF3707 and GFF3708 overlap by 11 nucleotides GFF3708 and GFF3709 are separated by 31 nucleotides GFF3709 and GFF3710 are separated by 85 nucleotides
GFF3706 - Peptidyl-prolyl cis-trans isomerase PpiA precursor (EC 5.2.1.8), at 13,147 to 13,719
GFF3706
GFF3707 - FIG00639558: hypothetical protein, at 13,828 to 13,995
GFF3707
GFF3708 - Cell filamentation protein fic, at 13,985 to 14,587
GFF3708
GFF3709 - Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85), at 14,619 to 15,182
GFF3709
GFF3710 - Acetylornithine aminotransferase (EC 2.6.1.11) / N-succinyl-L,L-diaminopimelate aminotransferase (EC 2.6.1.17), at 15,268 to 16,485
GFF3710
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1 at 13.788 kb on + strand at 13.791 kb on - strand at 13.791 kb on - strand at 13.915 kb on - strand, within GFF3707 at 13.915 kb on - strand, within GFF3707 at 13.928 kb on + strand, within GFF3707 at 13.931 kb on + strand, within GFF3707 at 13.984 kb on + strand at 14.184 kb on - strand, within GFF3708 at 14.364 kb on - strand, within GFF3708 at 14.364 kb on - strand, within GFF3708 at 14.366 kb on + strand, within GFF3708 at 14.366 kb on + strand, within GFF3708 at 14.367 kb on - strand, within GFF3708 at 14.367 kb on - strand, within GFF3708 at 14.378 kb on - strand, within GFF3708 at 14.476 kb on + strand, within GFF3708 at 14.477 kb on - strand, within GFF3708 at 14.640 kb on + strand at 14.651 kb on + strand at 14.946 kb on - strand, within GFF3709 at 15.329 kb on - strand at 15.360 kb on + strand at 15.397 kb on + strand, within GFF3710 at 15.397 kb on + strand, within GFF3710 at 15.398 kb on - strand, within GFF3710 at 15.495 kb on + strand, within GFF3710 at 15.579 kb on + strand, within GFF3710 at 15.771 kb on + strand, within GFF3710 at 15.988 kb on + strand, within GFF3710 at 15.990 kb on + strand, within GFF3710 at 15.990 kb on + strand, within GFF3710 at 15.990 kb on + strand, within GFF3710 at 15.991 kb on - strand, within GFF3710 at 16.100 kb on + strand, within GFF3710 at 16.100 kb on + strand, within GFF3710 at 16.101 kb on - strand, within GFF3710 at 16.168 kb on - strand, within GFF3710 at 16.174 kb on - strand, within GFF3710 at 16.174 kb on - strand, within GFF3710
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 13,788 + -0.4 13,791 - +0.8 13,791 - -0.3 13,915 - GFF3707 0.52 -1.0 13,915 - GFF3707 0.52 +0.5 13,928 + GFF3707 0.60 +0.2 13,931 + GFF3707 0.61 +0.1 13,984 + -0.1 14,184 - GFF3708 0.33 -0.0 14,364 - GFF3708 0.63 -0.2 14,364 - GFF3708 0.63 +0.2 14,366 + GFF3708 0.63 -0.0 14,366 + GFF3708 0.63 -0.4 14,367 - GFF3708 0.63 +0.4 14,367 - GFF3708 0.63 -0.2 14,378 - GFF3708 0.65 -0.5 14,476 + GFF3708 0.81 -1.8 14,477 - GFF3708 0.82 +0.9 14,640 + +0.1 14,651 + +0.2 14,946 - GFF3709 0.58 +0.6 15,329 - -0.6 15,360 + +1.2 15,397 + GFF3710 0.11 -0.5 15,397 + GFF3710 0.11 +0.4 15,398 - GFF3710 0.11 -0.2 15,495 + GFF3710 0.19 -1.3 15,579 + GFF3710 0.26 -0.3 15,771 + GFF3710 0.41 +0.4 15,988 + GFF3710 0.59 +0.4 15,990 + GFF3710 0.59 -1.4 15,990 + GFF3710 0.59 -0.1 15,990 + GFF3710 0.59 +0.1 15,991 - GFF3710 0.59 -0.9 16,100 + GFF3710 0.68 +0.0 16,100 + GFF3710 0.68 +0.3 16,101 - GFF3710 0.68 -0.7 16,168 - GFF3710 0.74 -0.9 16,174 - GFF3710 0.74 +0.0 16,174 - GFF3710 0.74 -0.3
Or see this region's nucleotide sequence