Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3681 and GFF3682 are separated by 344 nucleotides GFF3682 and GFF3683 are separated by 259 nucleotides GFF3683 and GFF3684 are separated by 27 nucleotides
GFF3681 - Chromosome partition protein MukB, at 31,809 to 36,275
GFF3681
GFF3682 - L,D-transpeptidase YcbB, at 36,620 to 38,461
GFF3682
GFF3683 - FIG001587: exported protein, at 38,721 to 39,269
GFF3683
GFF3684 - Hypothetical metal-binding enzyme, YcbL homolog, at 39,297 to 39,944
GFF3684
Position (kb) 36
37
38
39 Strain fitness (log2 ratio) -2
-1
0
1 at 36.273 kb on + strand at 36.461 kb on + strand at 36.492 kb on + strand at 36.536 kb on + strand at 36.632 kb on - strand at 36.632 kb on - strand at 36.679 kb on - strand at 36.881 kb on - strand, within GFF3682 at 36.890 kb on + strand, within GFF3682 at 36.890 kb on + strand, within GFF3682 at 37.101 kb on - strand, within GFF3682 at 37.146 kb on + strand, within GFF3682 at 37.149 kb on - strand, within GFF3682 at 37.150 kb on + strand, within GFF3682 at 37.150 kb on + strand, within GFF3682 at 37.151 kb on - strand, within GFF3682 at 37.151 kb on - strand, within GFF3682 at 37.151 kb on - strand, within GFF3682 at 37.272 kb on - strand, within GFF3682 at 37.563 kb on - strand, within GFF3682 at 37.686 kb on + strand, within GFF3682 at 37.744 kb on + strand, within GFF3682 at 37.750 kb on - strand, within GFF3682 at 37.967 kb on + strand, within GFF3682 at 38.133 kb on - strand, within GFF3682 at 38.210 kb on - strand, within GFF3682 at 38.210 kb on - strand, within GFF3682 at 38.231 kb on - strand, within GFF3682 at 38.339 kb on + strand at 38.339 kb on + strand at 38.372 kb on - strand at 38.372 kb on - strand at 38.372 kb on - strand at 38.393 kb on + strand at 38.394 kb on - strand at 38.394 kb on - strand at 38.400 kb on - strand at 38.451 kb on + strand at 38.482 kb on - strand at 38.482 kb on - strand at 38.607 kb on - strand at 38.608 kb on + strand at 38.609 kb on - strand at 38.609 kb on - strand at 38.609 kb on - strand at 38.609 kb on - strand at 38.677 kb on + strand at 38.686 kb on - strand at 38.688 kb on + strand at 38.717 kb on + strand at 38.739 kb on - strand at 38.901 kb on + strand, within GFF3683 at 39.079 kb on + strand, within GFF3683 at 39.090 kb on - strand, within GFF3683 at 39.119 kb on + strand, within GFF3683 at 39.119 kb on + strand, within GFF3683 at 39.146 kb on + strand, within GFF3683 at 39.191 kb on + strand, within GFF3683 at 39.225 kb on - strand at 39.225 kb on - strand at 39.227 kb on + strand at 39.303 kb on + strand at 39.309 kb on - strand at 39.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 36,273 + -0.1 36,461 + +1.4 36,492 + +0.2 36,536 + -0.0 36,632 - -1.1 36,632 - +0.5 36,679 - +1.1 36,881 - GFF3682 0.14 +0.1 36,890 + GFF3682 0.15 -0.1 36,890 + GFF3682 0.15 -0.6 37,101 - GFF3682 0.26 -0.2 37,146 + GFF3682 0.29 +0.2 37,149 - GFF3682 0.29 +0.1 37,150 + GFF3682 0.29 -0.0 37,150 + GFF3682 0.29 -1.9 37,151 - GFF3682 0.29 +0.5 37,151 - GFF3682 0.29 +0.2 37,151 - GFF3682 0.29 +1.4 37,272 - GFF3682 0.35 -0.5 37,563 - GFF3682 0.51 +0.6 37,686 + GFF3682 0.58 +0.4 37,744 + GFF3682 0.61 +1.2 37,750 - GFF3682 0.61 +0.1 37,967 + GFF3682 0.73 +0.9 38,133 - GFF3682 0.82 +0.0 38,210 - GFF3682 0.86 +0.1 38,210 - GFF3682 0.86 +1.4 38,231 - GFF3682 0.87 +0.9 38,339 + +1.0 38,339 + -1.5 38,372 - +0.6 38,372 - -1.2 38,372 - +0.8 38,393 + +0.8 38,394 - +0.8 38,394 - +0.6 38,400 - -0.2 38,451 + +0.4 38,482 - +0.6 38,482 - -0.3 38,607 - +0.4 38,608 + -0.1 38,609 - +0.3 38,609 - +0.8 38,609 - -1.0 38,609 - +0.3 38,677 + +0.6 38,686 - +1.1 38,688 + -0.6 38,717 + +0.2 38,739 - +0.6 38,901 + GFF3683 0.33 +1.4 39,079 + GFF3683 0.65 +0.3 39,090 - GFF3683 0.67 +1.0 39,119 + GFF3683 0.72 +0.7 39,119 + GFF3683 0.72 +0.5 39,146 + GFF3683 0.77 +0.6 39,191 + GFF3683 0.86 +1.0 39,225 - +0.8 39,225 - +0.8 39,227 + +1.3 39,303 + -0.7 39,309 - -0.9 39,312 - +1.2
Or see this region's nucleotide sequence