Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3564 and GFF3565 are separated by 66 nucleotides GFF3565 and GFF3566 are separated by 82 nucleotides GFF3566 and GFF3567 are separated by 310 nucleotides GFF3567 and GFF3568 are separated by 401 nucleotides
GFF3564 - L,D-transpeptidase YnhG, at 320,042 to 321,043
GFF3564
GFF3565 - major outer membrane lipoprotein, at 321,110 to 321,349
GFF3565
GFF3566 - major outer membrane lipoprotein, at 321,432 to 321,668
GFF3566
GFF3567 - Pyruvate kinase (EC 2.7.1.40), at 321,979 to 323,391
GFF3567
GFF3568 - Amino acid transporter, at 323,793 to 325,136
GFF3568
Position (kb) 321
322
323
324 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 321.431 kb on - strand at 321.443 kb on - strand at 321.594 kb on - strand, within GFF3566 at 321.624 kb on - strand, within GFF3566 at 321.624 kb on - strand, within GFF3566 at 321.670 kb on - strand at 321.710 kb on - strand at 321.716 kb on - strand at 321.719 kb on - strand at 321.767 kb on - strand at 321.857 kb on + strand at 322.166 kb on - strand, within GFF3567 at 322.227 kb on + strand, within GFF3567 at 322.342 kb on - strand, within GFF3567 at 322.542 kb on + strand, within GFF3567 at 322.543 kb on - strand, within GFF3567 at 322.543 kb on - strand, within GFF3567 at 322.741 kb on - strand, within GFF3567 at 322.741 kb on - strand, within GFF3567 at 322.959 kb on + strand, within GFF3567 at 322.972 kb on - strand, within GFF3567 at 323.063 kb on + strand, within GFF3567 at 323.063 kb on + strand, within GFF3567 at 323.193 kb on + strand, within GFF3567 at 323.367 kb on + strand at 323.368 kb on - strand at 323.460 kb on - strand at 323.474 kb on - strand at 323.474 kb on - strand at 323.500 kb on + strand at 323.500 kb on + strand at 323.501 kb on - strand at 323.585 kb on - strand at 323.630 kb on + strand at 323.631 kb on - strand at 323.707 kb on + strand at 323.711 kb on - strand at 323.711 kb on - strand at 323.899 kb on - strand at 324.080 kb on + strand, within GFF3568 at 324.161 kb on + strand, within GFF3568 at 324.255 kb on - strand, within GFF3568 at 324.255 kb on - strand, within GFF3568
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 321,431 - -3.0 321,443 - -3.0 321,594 - GFF3566 0.68 -4.6 321,624 - GFF3566 0.81 -3.1 321,624 - GFF3566 0.81 -0.4 321,670 - -2.1 321,710 - -3.5 321,716 - -3.6 321,719 - -0.1 321,767 - -0.8 321,857 + -0.4 322,166 - GFF3567 0.13 +3.2 322,227 + GFF3567 0.18 +2.7 322,342 - GFF3567 0.26 +3.4 322,542 + GFF3567 0.40 +3.2 322,543 - GFF3567 0.40 +2.8 322,543 - GFF3567 0.40 +3.4 322,741 - GFF3567 0.54 +3.7 322,741 - GFF3567 0.54 +3.3 322,959 + GFF3567 0.69 +3.4 322,972 - GFF3567 0.70 +3.5 323,063 + GFF3567 0.77 +3.7 323,063 + GFF3567 0.77 +3.0 323,193 + GFF3567 0.86 +3.1 323,367 + +2.1 323,368 - +3.3 323,460 - +0.1 323,474 - -0.2 323,474 - +0.3 323,500 + +2.9 323,500 + +2.5 323,501 - +0.5 323,585 - +1.7 323,630 + +2.6 323,631 - +2.5 323,707 + +0.5 323,711 - +0.0 323,711 - +0.6 323,899 - +0.8 324,080 + GFF3568 0.21 +0.6 324,161 + GFF3568 0.27 -0.4 324,255 - GFF3568 0.34 +0.1 324,255 - GFF3568 0.34 -0.4
Or see this region's nucleotide sequence