Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3537 and GFF3538 are separated by 176 nucleotides GFF3538 and GFF3539 are separated by 46 nucleotides GFF3539 and GFF3540 are separated by 41 nucleotides
GFF3537 - FIG137360: hypothetical protein, at 288,653 to 289,486
GFF3537
GFF3538 - hypothetical protein, at 289,663 to 289,776
GFF3538
GFF3539 - Phosphoenolpyruvate synthase (EC 2.7.9.2), at 289,823 to 292,201
GFF3539
GFF3540 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 292,243 to 293,883
GFF3540
Position (kb) 289
290
291
292
293 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 288.881 kb on - strand, within GFF3537 at 288.887 kb on + strand, within GFF3537 at 288.919 kb on + strand, within GFF3537 at 288.950 kb on - strand, within GFF3537 at 289.110 kb on + strand, within GFF3537 at 289.454 kb on - strand at 289.756 kb on + strand, within GFF3538 at 289.770 kb on + strand at 289.778 kb on + strand at 290.054 kb on - strand at 290.267 kb on - strand, within GFF3539 at 290.363 kb on + strand, within GFF3539 at 290.546 kb on + strand, within GFF3539 at 290.547 kb on - strand, within GFF3539 at 290.759 kb on + strand, within GFF3539 at 290.760 kb on - strand, within GFF3539 at 290.815 kb on + strand, within GFF3539 at 290.871 kb on - strand, within GFF3539 at 290.871 kb on - strand, within GFF3539 at 290.872 kb on + strand, within GFF3539 at 291.250 kb on - strand, within GFF3539 at 291.342 kb on + strand, within GFF3539 at 291.370 kb on + strand, within GFF3539 at 291.377 kb on - strand, within GFF3539 at 291.608 kb on + strand, within GFF3539 at 291.928 kb on + strand, within GFF3539 at 291.928 kb on + strand, within GFF3539 at 292.062 kb on - strand at 292.199 kb on + strand at 292.205 kb on - strand at 292.205 kb on - strand at 292.278 kb on + strand at 292.279 kb on - strand at 292.367 kb on + strand at 292.367 kb on + strand at 292.448 kb on + strand, within GFF3540 at 292.448 kb on + strand, within GFF3540 at 292.449 kb on - strand, within GFF3540 at 292.486 kb on - strand, within GFF3540 at 292.530 kb on + strand, within GFF3540 at 292.644 kb on - strand, within GFF3540 at 292.704 kb on + strand, within GFF3540 at 292.884 kb on - strand, within GFF3540 at 292.884 kb on - strand, within GFF3540 at 292.941 kb on + strand, within GFF3540 at 293.050 kb on - strand, within GFF3540 at 293.069 kb on - strand, within GFF3540
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 288,881 - GFF3537 0.27 -0.4 288,887 + GFF3537 0.28 +0.6 288,919 + GFF3537 0.32 +0.2 288,950 - GFF3537 0.36 -0.3 289,110 + GFF3537 0.55 -0.1 289,454 - +0.6 289,756 + GFF3538 0.82 -0.2 289,770 + +0.5 289,778 + +0.7 290,054 - +1.0 290,267 - GFF3539 0.19 +0.6 290,363 + GFF3539 0.23 -3.0 290,546 + GFF3539 0.30 -1.2 290,547 - GFF3539 0.30 +0.5 290,759 + GFF3539 0.39 -1.3 290,760 - GFF3539 0.39 -0.2 290,815 + GFF3539 0.42 -0.2 290,871 - GFF3539 0.44 +0.6 290,871 - GFF3539 0.44 +0.1 290,872 + GFF3539 0.44 -0.2 291,250 - GFF3539 0.60 +0.2 291,342 + GFF3539 0.64 -1.4 291,370 + GFF3539 0.65 -2.0 291,377 - GFF3539 0.65 -1.1 291,608 + GFF3539 0.75 +0.5 291,928 + GFF3539 0.88 +0.8 291,928 + GFF3539 0.88 +0.8 292,062 - +0.2 292,199 + -0.2 292,205 - -1.4 292,205 - +0.6 292,278 + +0.6 292,279 - -1.2 292,367 + +0.4 292,367 + -0.0 292,448 + GFF3540 0.12 +2.2 292,448 + GFF3540 0.12 -0.5 292,449 - GFF3540 0.13 +0.7 292,486 - GFF3540 0.15 +0.7 292,530 + GFF3540 0.17 -0.3 292,644 - GFF3540 0.24 +0.6 292,704 + GFF3540 0.28 -0.3 292,884 - GFF3540 0.39 -0.1 292,884 - GFF3540 0.39 +0.1 292,941 + GFF3540 0.43 +0.5 293,050 - GFF3540 0.49 -0.1 293,069 - GFF3540 0.50 -0.0
Or see this region's nucleotide sequence