Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3477 and GFF3478 are separated by 334 nucleotides GFF3478 and GFF3479 are separated by 341 nucleotides GFF3479 and GFF3480 are separated by 41 nucleotides
GFF3477 - Aldose 1-epimerase family protein YeaD, at 229,527 to 230,411
GFF3477
GFF3478 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 230,746 to 231,741
GFF3478
GFF3479 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 232,083 to 232,496
GFF3479
GFF3480 - Uncharacterized protein YeaC, at 232,538 to 232,816
GFF3480
Position (kb) 230
231
232 Strain fitness (log2 ratio) -2
-1
0
1 at 229.812 kb on + strand, within GFF3477 at 229.812 kb on + strand, within GFF3477 at 230.067 kb on + strand, within GFF3477 at 230.068 kb on - strand, within GFF3477 at 230.226 kb on - strand, within GFF3477 at 230.238 kb on + strand, within GFF3477 at 230.286 kb on + strand, within GFF3477 at 230.425 kb on + strand at 230.565 kb on - strand at 230.569 kb on - strand at 230.685 kb on - strand at 231.943 kb on + strand at 231.943 kb on + strand at 231.946 kb on + strand at 231.947 kb on - strand at 232.176 kb on - strand, within GFF3479 at 232.193 kb on + strand, within GFF3479 at 232.320 kb on + strand, within GFF3479 at 232.320 kb on + strand, within GFF3479 at 232.324 kb on + strand, within GFF3479 at 232.325 kb on - strand, within GFF3479 at 232.439 kb on + strand, within GFF3479 at 232.580 kb on + strand, within GFF3480 at 232.580 kb on + strand, within GFF3480 at 232.612 kb on + strand, within GFF3480 at 232.677 kb on + strand, within GFF3480 at 232.711 kb on + strand, within GFF3480 at 232.712 kb on - strand, within GFF3480 at 232.712 kb on - strand, within GFF3480
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 229,812 + GFF3477 0.32 +0.6 229,812 + GFF3477 0.32 -0.4 230,067 + GFF3477 0.61 +0.9 230,068 - GFF3477 0.61 -0.8 230,226 - GFF3477 0.79 +0.5 230,238 + GFF3477 0.80 -0.8 230,286 + GFF3477 0.86 -0.3 230,425 + -0.0 230,565 - +0.2 230,569 - -0.4 230,685 - -2.2 231,943 + -1.2 231,943 + -0.3 231,946 + -0.3 231,947 - -0.6 232,176 - GFF3479 0.22 +0.4 232,193 + GFF3479 0.27 +0.8 232,320 + GFF3479 0.57 -0.0 232,320 + GFF3479 0.57 +1.6 232,324 + GFF3479 0.58 -0.1 232,325 - GFF3479 0.58 -0.5 232,439 + GFF3479 0.86 +0.3 232,580 + GFF3480 0.15 -1.6 232,580 + GFF3480 0.15 -0.7 232,612 + GFF3480 0.27 -0.5 232,677 + GFF3480 0.50 -0.6 232,711 + GFF3480 0.62 -1.6 232,712 - GFF3480 0.62 -0.4 232,712 - GFF3480 0.62 -0.0
Or see this region's nucleotide sequence