Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2991 and GFF2992 are separated by 112 nucleotides GFF2992 and GFF2993 are separated by 52 nucleotides GFF2993 and GFF2994 overlap by 20 nucleotides GFF2994 and GFF2995 overlap by 2 nucleotides GFF2995 and GFF2996 are separated by 14 nucleotides
GFF2991 - Tyrosine recombinase XerD, at 9,559 to 10,455
GFF2991
GFF2992 - Flavodoxin 2, at 10,568 to 11,089
GFF2992
GFF2993 - FIG00731769: possible membrane protein, at 11,142 to 11,555
GFF2993
GFF2994 - YgfY COG2938, at 11,536 to 11,802
GFF2994
GFF2995 - FIG01045243: hypothetical protein, at 11,801 to 12,037
GFF2995
GFF2996 - Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress, at 12,052 to 13,032
GFF2996
Position (kb)
11
12 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 10.491 kb on + strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.543 kb on + strand at 10.561 kb on + strand at 10.562 kb on - strand at 10.562 kb on - strand at 10.567 kb on - strand at 10.567 kb on - strand at 10.604 kb on + strand at 10.604 kb on + strand at 10.662 kb on + strand, within GFF2992 at 10.662 kb on + strand, within GFF2992 at 10.662 kb on + strand, within GFF2992 at 10.663 kb on - strand, within GFF2992 at 10.663 kb on - strand, within GFF2992 at 10.663 kb on - strand, within GFF2992 at 10.663 kb on - strand, within GFF2992 at 10.703 kb on + strand, within GFF2992 at 10.873 kb on + strand, within GFF2992 at 10.907 kb on - strand, within GFF2992 at 10.954 kb on + strand, within GFF2992 at 11.035 kb on + strand, within GFF2992 at 11.036 kb on - strand, within GFF2992 at 11.105 kb on - strand at 11.170 kb on - strand at 11.287 kb on - strand, within GFF2993 at 11.375 kb on + strand, within GFF2993 at 11.375 kb on + strand, within GFF2993 at 11.376 kb on - strand, within GFF2993 at 11.390 kb on + strand, within GFF2993 at 11.422 kb on - strand, within GFF2993 at 11.422 kb on - strand, within GFF2993 at 11.422 kb on - strand, within GFF2993 at 11.424 kb on + strand, within GFF2993 at 11.424 kb on + strand, within GFF2993 at 11.425 kb on - strand, within GFF2993 at 11.444 kb on - strand, within GFF2993 at 11.739 kb on + strand, within GFF2994 at 11.779 kb on - strand at 11.787 kb on + strand at 11.796 kb on + strand at 11.797 kb on - strand at 11.842 kb on + strand, within GFF2995 at 11.920 kb on - strand, within GFF2995 at 11.981 kb on - strand, within GFF2995 at 11.981 kb on - strand, within GFF2995 at 12.035 kb on + strand at 12.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 10,491 + +1.4 10,492 - +0.2 10,492 - +0.7 10,492 - -0.9 10,492 - -0.1 10,543 + +0.5 10,561 + +1.8 10,562 - +2.6 10,562 - +4.1 10,567 - +3.2 10,567 - +3.4 10,604 + +2.5 10,604 + +2.8 10,662 + GFF2992 0.18 +3.0 10,662 + GFF2992 0.18 +3.5 10,662 + GFF2992 0.18 +2.8 10,663 - GFF2992 0.18 +2.9 10,663 - GFF2992 0.18 +2.9 10,663 - GFF2992 0.18 +3.1 10,663 - GFF2992 0.18 +2.7 10,703 + GFF2992 0.26 +3.0 10,873 + GFF2992 0.58 +3.0 10,907 - GFF2992 0.65 +1.7 10,954 + GFF2992 0.74 +3.4 11,035 + GFF2992 0.89 +3.3 11,036 - GFF2992 0.90 +1.1 11,105 - -0.9 11,170 - +1.1 11,287 - GFF2993 0.35 +1.7 11,375 + GFF2993 0.56 +2.3 11,375 + GFF2993 0.56 +0.1 11,376 - GFF2993 0.57 +1.9 11,390 + GFF2993 0.60 +0.8 11,422 - GFF2993 0.68 +1.4 11,422 - GFF2993 0.68 -1.0 11,422 - GFF2993 0.68 +2.7 11,424 + GFF2993 0.68 +0.9 11,424 + GFF2993 0.68 +1.3 11,425 - GFF2993 0.68 +1.9 11,444 - GFF2993 0.73 +1.3 11,739 + GFF2994 0.76 -0.5 11,779 - -1.2 11,787 + +0.1 11,796 + +0.1 11,797 - -0.4 11,842 + GFF2995 0.17 +0.9 11,920 - GFF2995 0.50 -0.7 11,981 - GFF2995 0.76 +1.2 11,981 - GFF2995 0.76 +0.9 12,035 + +1.5 12,047 + +2.8
Or see this region's nucleotide sequence