Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2892 and GFF2893 are separated by 118 nucleotides GFF2893 and GFF2894 are separated by 39 nucleotides
GFF2892 - ATP-dependent RNA helicase RhlB, at 16,498 to 17,763
GFF2892
GFF2893 - 'Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (EC 3.6.1.40) @ Exopolyphosphatase (EC 3.6.1.11)' transl_table=11, at 17,882 to 19,363
GFF2893
GFF2894 - ATP-dependent DNA helicase Rep, at 19,403 to 21,427
GFF2894
Position (kb)
17
18
19
20 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 16.912 kb on - strand, within GFF2892 at 16.917 kb on + strand, within GFF2892 at 17.022 kb on + strand, within GFF2892 at 17.052 kb on + strand, within GFF2892 at 17.207 kb on - strand, within GFF2892 at 17.234 kb on + strand, within GFF2892 at 17.234 kb on + strand, within GFF2892 at 17.310 kb on + strand, within GFF2892 at 17.484 kb on + strand, within GFF2892 at 17.484 kb on + strand, within GFF2892 at 17.489 kb on - strand, within GFF2892 at 17.516 kb on - strand, within GFF2892 at 17.587 kb on + strand, within GFF2892 at 17.617 kb on + strand, within GFF2892 at 17.772 kb on - strand at 17.792 kb on + strand at 17.792 kb on + strand at 17.799 kb on + strand at 17.800 kb on - strand at 17.834 kb on + strand at 17.834 kb on + strand at 17.843 kb on + strand at 17.844 kb on - strand at 17.882 kb on - strand at 17.901 kb on - strand at 17.986 kb on - strand at 18.184 kb on + strand, within GFF2893 at 18.208 kb on + strand, within GFF2893 at 18.208 kb on + strand, within GFF2893 at 18.404 kb on - strand, within GFF2893 at 18.571 kb on + strand, within GFF2893 at 18.595 kb on - strand, within GFF2893 at 18.595 kb on - strand, within GFF2893 at 18.838 kb on + strand, within GFF2893 at 18.838 kb on + strand, within GFF2893 at 18.883 kb on + strand, within GFF2893 at 18.916 kb on + strand, within GFF2893 at 18.926 kb on - strand, within GFF2893 at 19.017 kb on + strand, within GFF2893 at 19.018 kb on - strand, within GFF2893 at 19.021 kb on - strand, within GFF2893 at 19.080 kb on + strand, within GFF2893 at 19.080 kb on + strand, within GFF2893 at 19.158 kb on + strand, within GFF2893 at 19.159 kb on - strand, within GFF2893 at 19.334 kb on + strand at 19.335 kb on - strand at 19.335 kb on - strand at 19.335 kb on - strand at 19.338 kb on + strand at 19.338 kb on + strand at 19.338 kb on + strand at 19.338 kb on + strand at 19.367 kb on + strand at 19.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 16,912 - GFF2892 0.33 +3.5 16,917 + GFF2892 0.33 +4.0 17,022 + GFF2892 0.41 +3.6 17,052 + GFF2892 0.44 +3.2 17,207 - GFF2892 0.56 +4.1 17,234 + GFF2892 0.58 +2.9 17,234 + GFF2892 0.58 +2.9 17,310 + GFF2892 0.64 +3.0 17,484 + GFF2892 0.78 +2.2 17,484 + GFF2892 0.78 +2.6 17,489 - GFF2892 0.78 +3.9 17,516 - GFF2892 0.80 +4.1 17,587 + GFF2892 0.86 +1.4 17,617 + GFF2892 0.88 +2.7 17,772 - +3.6 17,792 + +3.2 17,792 + +1.8 17,799 + +2.6 17,800 - +3.6 17,834 + +1.6 17,834 + +1.1 17,843 + +2.8 17,844 - +3.4 17,882 - +3.7 17,901 - +3.8 17,986 - +3.7 18,184 + GFF2893 0.20 +3.9 18,208 + GFF2893 0.22 +3.5 18,208 + GFF2893 0.22 +3.2 18,404 - GFF2893 0.35 +3.7 18,571 + GFF2893 0.46 +3.6 18,595 - GFF2893 0.48 +3.8 18,595 - GFF2893 0.48 +2.3 18,838 + GFF2893 0.65 +3.6 18,838 + GFF2893 0.65 +2.7 18,883 + GFF2893 0.68 +3.7 18,916 + GFF2893 0.70 +3.9 18,926 - GFF2893 0.70 +4.0 19,017 + GFF2893 0.77 +3.6 19,018 - GFF2893 0.77 +4.7 19,021 - GFF2893 0.77 +3.6 19,080 + GFF2893 0.81 +2.2 19,080 + GFF2893 0.81 +3.5 19,158 + GFF2893 0.86 +3.1 19,159 - GFF2893 0.86 +3.9 19,334 + +3.6 19,335 - +3.9 19,335 - +3.5 19,335 - +3.6 19,338 + +3.6 19,338 + +3.5 19,338 + +3.8 19,338 + +3.3 19,367 + +1.2 19,445 - -1.1
Or see this region's nucleotide sequence