Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF271 and GFF272 are separated by 196 nucleotides GFF272 and GFF273 are separated by 9 nucleotides
GFF271 - Possible transferase, at 45,701 to 46,774
GFF271
GFF272 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 46,971 to 48,134
GFF272
GFF273 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 48,144 to 50,333
GFF273
Position (kb) 46
47
48
49 Strain fitness (log2 ratio) -1
0
1
2 at 46.012 kb on + strand, within GFF271 at 46.013 kb on - strand, within GFF271 at 46.118 kb on + strand, within GFF271 at 46.357 kb on + strand, within GFF271 at 46.358 kb on - strand, within GFF271 at 46.360 kb on + strand, within GFF271 at 46.360 kb on + strand, within GFF271 at 46.361 kb on - strand, within GFF271 at 46.376 kb on - strand, within GFF271 at 46.380 kb on + strand, within GFF271 at 46.380 kb on + strand, within GFF271 at 46.627 kb on - strand, within GFF271 at 46.654 kb on + strand, within GFF271 at 46.713 kb on + strand at 46.713 kb on + strand at 46.713 kb on + strand at 46.738 kb on + strand at 46.738 kb on + strand at 46.828 kb on + strand at 46.893 kb on + strand at 46.893 kb on + strand at 46.893 kb on + strand at 46.894 kb on - strand at 46.894 kb on - strand at 46.894 kb on - strand at 46.894 kb on - strand at 46.894 kb on - strand at 46.897 kb on + strand at 46.897 kb on + strand at 46.897 kb on + strand at 46.897 kb on + strand at 46.897 kb on + strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.901 kb on + strand at 46.902 kb on - strand at 46.902 kb on - strand at 46.902 kb on - strand at 46.962 kb on + strand at 46.963 kb on - strand at 47.015 kb on + strand at 47.016 kb on - strand at 47.016 kb on - strand at 47.385 kb on + strand, within GFF272 at 47.519 kb on - strand, within GFF272 at 47.519 kb on - strand, within GFF272 at 47.925 kb on - strand, within GFF272 at 47.978 kb on + strand, within GFF272 at 47.979 kb on - strand, within GFF272 at 47.999 kb on + strand, within GFF272 at 48.011 kb on + strand, within GFF272 at 48.026 kb on + strand at 48.107 kb on + strand at 48.145 kb on + strand at 48.197 kb on - strand at 48.244 kb on + strand at 48.266 kb on - strand at 48.368 kb on + strand, within GFF273 at 48.443 kb on + strand, within GFF273 at 48.453 kb on + strand, within GFF273
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 46,012 + GFF271 0.29 +1.1 46,013 - GFF271 0.29 +0.4 46,118 + GFF271 0.39 +0.0 46,357 + GFF271 0.61 +0.3 46,358 - GFF271 0.61 +0.2 46,360 + GFF271 0.61 -0.5 46,360 + GFF271 0.61 +0.3 46,361 - GFF271 0.61 +0.1 46,376 - GFF271 0.63 -1.5 46,380 + GFF271 0.63 -0.9 46,380 + GFF271 0.63 -0.0 46,627 - GFF271 0.86 +0.0 46,654 + GFF271 0.89 -0.2 46,713 + +0.4 46,713 + -0.3 46,713 + -0.2 46,738 + +0.9 46,738 + -0.4 46,828 + +0.5 46,893 + +1.2 46,893 + -0.6 46,893 + +0.2 46,894 - +0.8 46,894 - +0.9 46,894 - +0.9 46,894 - +1.9 46,894 - +0.1 46,897 + -0.5 46,897 + -0.2 46,897 + +0.3 46,897 + -0.3 46,897 + -0.1 46,898 - +0.3 46,898 - +0.0 46,898 - -0.2 46,898 - +0.2 46,898 - +0.2 46,898 - +0.4 46,898 - +0.3 46,901 + +0.2 46,902 - -0.7 46,902 - -1.7 46,902 - -0.1 46,962 + -0.5 46,963 - +0.2 47,015 + +0.6 47,016 - +0.2 47,016 - +1.6 47,385 + GFF272 0.36 +0.1 47,519 - GFF272 0.47 +0.3 47,519 - GFF272 0.47 -0.2 47,925 - GFF272 0.82 +0.9 47,978 + GFF272 0.87 +0.7 47,979 - GFF272 0.87 +0.1 47,999 + GFF272 0.88 +0.4 48,011 + GFF272 0.89 -0.0 48,026 + -0.4 48,107 + -1.5 48,145 + -0.4 48,197 - -0.2 48,244 + +0.6 48,266 - +0.6 48,368 + GFF273 0.10 -0.6 48,443 + GFF273 0.14 -0.1 48,453 + GFF273 0.14 -0.3
Or see this region's nucleotide sequence