Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2427 and GFF2428 are separated by 139 nucleotides GFF2428 and GFF2429 are separated by 246 nucleotides GFF2429 and GFF2430 are separated by 11 nucleotides GFF2430 and GFF2431 are separated by 1 nucleotides
GFF2427 - Protein SseB, at 43,716 to 44,462
GFF2427
GFF2428 - Peptidase B (EC 3.4.11.23), at 44,602 to 45,885
GFF2428
GFF2429 - Believed to be involved in assembly of Fe-S clusters, at 46,132 to 46,332
GFF2429
GFF2430 - Ferredoxin, 2Fe-2S, at 46,344 to 46,679
GFF2430
GFF2431 - Chaperone protein HscA, at 46,681 to 48,531
GFF2431
Position (kb) 44
45
46 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 43.677 kb on + strand at 43.796 kb on + strand, within GFF2427 at 44.016 kb on - strand, within GFF2427 at 44.061 kb on + strand, within GFF2427 at 44.151 kb on - strand, within GFF2427 at 44.285 kb on + strand, within GFF2427 at 44.506 kb on + strand at 44.511 kb on - strand at 44.511 kb on - strand at 44.521 kb on + strand at 44.910 kb on + strand, within GFF2428 at 44.936 kb on + strand, within GFF2428 at 44.993 kb on - strand, within GFF2428 at 44.993 kb on - strand, within GFF2428 at 45.037 kb on + strand, within GFF2428 at 45.082 kb on - strand, within GFF2428 at 45.100 kb on - strand, within GFF2428 at 45.114 kb on + strand, within GFF2428 at 45.298 kb on - strand, within GFF2428 at 45.320 kb on + strand, within GFF2428 at 45.416 kb on + strand, within GFF2428 at 45.544 kb on - strand, within GFF2428 at 45.793 kb on - strand at 45.793 kb on - strand at 45.815 kb on + strand at 45.944 kb on - strand at 46.063 kb on + strand at 46.071 kb on + strand at 46.071 kb on + strand at 46.071 kb on + strand at 46.287 kb on - strand, within GFF2429 at 46.332 kb on + strand at 46.336 kb on - strand at 46.516 kb on - strand, within GFF2430 at 46.769 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 43,677 + +0.1 43,796 + GFF2427 0.11 -0.3 44,016 - GFF2427 0.40 -0.4 44,061 + GFF2427 0.46 +0.7 44,151 - GFF2427 0.58 +0.6 44,285 + GFF2427 0.76 +0.7 44,506 + +0.7 44,511 - +0.4 44,511 - -0.5 44,521 + +0.4 44,910 + GFF2428 0.24 +0.9 44,936 + GFF2428 0.26 +0.1 44,993 - GFF2428 0.30 +0.7 44,993 - GFF2428 0.30 +0.2 45,037 + GFF2428 0.34 +0.3 45,082 - GFF2428 0.37 +0.8 45,100 - GFF2428 0.39 -0.2 45,114 + GFF2428 0.40 +1.8 45,298 - GFF2428 0.54 +0.3 45,320 + GFF2428 0.56 +0.2 45,416 + GFF2428 0.63 +0.1 45,544 - GFF2428 0.73 -0.3 45,793 - -1.0 45,793 - +0.5 45,815 + +0.6 45,944 - -0.1 46,063 + +0.8 46,071 + +0.7 46,071 + +0.4 46,071 + -0.6 46,287 - GFF2429 0.77 -2.2 46,332 + -0.0 46,336 - -3.2 46,516 - GFF2430 0.51 -5.0 46,769 - -5.2
Or see this region's nucleotide sequence