Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2414 and GFF2415 are separated by 206 nucleotides GFF2415 and GFF2416 are separated by 120 nucleotides GFF2416 and GFF2417 overlap by 1 nucleotides
GFF2414 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 27,181 to 28,347
GFF2414
GFF2415 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 28,554 to 28,985
GFF2415
GFF2416 - Putative polyferredoxin, at 29,106 to 29,969
GFF2416
GFF2417 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 29,969 to 30,778
GFF2417
Position (kb) 28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 27.582 kb on - strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.635 kb on - strand, within GFF2414 at 27.635 kb on - strand, within GFF2414 at 27.658 kb on + strand, within GFF2414 at 27.658 kb on + strand, within GFF2414 at 27.788 kb on - strand, within GFF2414 at 28.014 kb on - strand, within GFF2414 at 28.124 kb on - strand, within GFF2414 at 28.188 kb on - strand, within GFF2414 at 28.188 kb on - strand, within GFF2414 at 28.347 kb on + strand at 28.347 kb on + strand at 28.347 kb on + strand at 28.347 kb on + strand at 28.348 kb on - strand at 28.370 kb on - strand at 28.376 kb on + strand at 28.419 kb on + strand at 28.461 kb on - strand at 28.511 kb on + strand at 28.658 kb on + strand, within GFF2415 at 28.659 kb on - strand, within GFF2415 at 28.659 kb on - strand, within GFF2415 at 28.659 kb on - strand, within GFF2415 at 28.773 kb on - strand, within GFF2415 at 28.834 kb on + strand, within GFF2415 at 28.834 kb on + strand, within GFF2415 at 28.990 kb on - strand at 29.031 kb on + strand at 29.094 kb on + strand at 29.094 kb on + strand at 29.207 kb on + strand, within GFF2416 at 29.208 kb on - strand, within GFF2416 at 29.217 kb on - strand, within GFF2416 at 29.220 kb on - strand, within GFF2416 at 29.349 kb on - strand, within GFF2416 at 29.649 kb on - strand, within GFF2416 at 29.811 kb on + strand, within GFF2416 at 29.812 kb on - strand, within GFF2416 at 29.825 kb on + strand, within GFF2416 at 29.977 kb on + strand at 29.980 kb on + strand at 29.981 kb on - strand at 29.981 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 27,582 - GFF2414 0.34 -0.2 27,634 + GFF2414 0.39 -0.0 27,634 + GFF2414 0.39 +0.9 27,634 + GFF2414 0.39 +0.1 27,634 + GFF2414 0.39 +1.0 27,635 - GFF2414 0.39 +0.7 27,635 - GFF2414 0.39 +0.3 27,658 + GFF2414 0.41 +0.1 27,658 + GFF2414 0.41 +0.1 27,788 - GFF2414 0.52 +1.3 28,014 - GFF2414 0.71 +0.8 28,124 - GFF2414 0.81 +1.0 28,188 - GFF2414 0.86 +0.5 28,188 - GFF2414 0.86 +0.7 28,347 + +0.6 28,347 + -0.4 28,347 + +2.2 28,347 + +0.2 28,348 - -0.2 28,370 - -0.0 28,376 + +0.5 28,419 + +0.7 28,461 - +1.4 28,511 + -0.0 28,658 + GFF2415 0.24 -0.3 28,659 - GFF2415 0.24 -3.4 28,659 - GFF2415 0.24 -1.7 28,659 - GFF2415 0.24 -2.0 28,773 - GFF2415 0.51 -0.2 28,834 + GFF2415 0.65 -2.2 28,834 + GFF2415 0.65 -2.5 28,990 - -2.7 29,031 + -1.6 29,094 + +0.3 29,094 + -3.6 29,207 + GFF2416 0.12 +1.8 29,208 - GFF2416 0.12 -0.2 29,217 - GFF2416 0.13 +0.2 29,220 - GFF2416 0.13 -0.8 29,349 - GFF2416 0.28 +0.8 29,649 - GFF2416 0.63 -0.5 29,811 + GFF2416 0.82 -0.5 29,812 - GFF2416 0.82 +0.1 29,825 + GFF2416 0.83 +0.2 29,977 + +0.2 29,980 + -0.3 29,981 - -0.6 29,981 - -0.1
Or see this region's nucleotide sequence