Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2118 and GFF2119 are separated by 72 nucleotides GFF2119 and GFF2120 are separated by 2 nucleotides GFF2120 and GFF2121 are separated by 150 nucleotides GFF2121 and GFF2122 are separated by 82 nucleotides
GFF2118 - FIG01046338: hypothetical protein, at 5,998 to 6,156
GFF2118
GFF2119 - Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family, at 6,229 to 6,891
GFF2119
GFF2120 - FIG00554530: hypothetical protein, at 6,894 to 8,993
GFF2120
GFF2121 - FIG00638667: hypothetical protein, at 9,144 to 9,308
GFF2121
GFF2122 - Adenine-specific methyltransferase (EC 2.1.1.72), at 9,391 to 10,275
GFF2122
Position (kb)
6
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1 at 6.405 kb on + strand, within GFF2119 at 6.896 kb on - strand at 6.909 kb on - strand at 7.054 kb on - strand at 7.080 kb on + strand at 7.198 kb on + strand, within GFF2120 at 7.213 kb on + strand, within GFF2120 at 7.434 kb on + strand, within GFF2120 at 7.435 kb on - strand, within GFF2120 at 7.603 kb on - strand, within GFF2120 at 7.607 kb on + strand, within GFF2120 at 7.608 kb on - strand, within GFF2120 at 7.771 kb on + strand, within GFF2120 at 7.771 kb on + strand, within GFF2120 at 7.881 kb on - strand, within GFF2120 at 7.881 kb on - strand, within GFF2120 at 7.894 kb on - strand, within GFF2120 at 8.066 kb on - strand, within GFF2120 at 8.202 kb on - strand, within GFF2120 at 8.267 kb on + strand, within GFF2120 at 8.293 kb on - strand, within GFF2120 at 8.312 kb on + strand, within GFF2120 at 8.322 kb on - strand, within GFF2120 at 8.442 kb on - strand, within GFF2120 at 8.445 kb on + strand, within GFF2120 at 8.552 kb on + strand, within GFF2120 at 8.626 kb on + strand, within GFF2120 at 8.626 kb on + strand, within GFF2120 at 8.626 kb on + strand, within GFF2120 at 8.868 kb on + strand at 8.869 kb on - strand at 8.916 kb on + strand at 8.916 kb on + strand at 8.927 kb on - strand at 9.057 kb on - strand at 9.117 kb on + strand at 9.299 kb on + strand at 9.303 kb on + strand at 9.303 kb on + strand at 9.303 kb on + strand at 9.304 kb on - strand at 9.348 kb on + strand at 9.349 kb on - strand at 9.350 kb on + strand at 9.653 kb on - strand, within GFF2122 at 9.702 kb on - strand, within GFF2122 at 9.716 kb on + strand, within GFF2122 at 9.717 kb on - strand, within GFF2122 at 9.767 kb on + strand, within GFF2122 at 9.897 kb on - strand, within GFF2122 at 9.900 kb on + strand, within GFF2122 at 9.900 kb on + strand, within GFF2122 at 9.936 kb on + strand, within GFF2122 at 9.937 kb on - strand, within GFF2122 at 9.937 kb on - strand, within GFF2122 at 9.941 kb on + strand, within GFF2122 at 9.941 kb on + strand, within GFF2122
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 6,405 + GFF2119 0.27 -0.7 6,896 - +0.0 6,909 - +0.3 7,054 - +0.2 7,080 + +0.7 7,198 + GFF2120 0.14 +0.2 7,213 + GFF2120 0.15 +0.4 7,434 + GFF2120 0.26 +0.5 7,435 - GFF2120 0.26 -0.6 7,603 - GFF2120 0.34 -0.1 7,607 + GFF2120 0.34 +1.7 7,608 - GFF2120 0.34 +0.4 7,771 + GFF2120 0.42 -2.0 7,771 + GFF2120 0.42 -0.1 7,881 - GFF2120 0.47 -0.3 7,881 - GFF2120 0.47 -0.3 7,894 - GFF2120 0.48 -0.7 8,066 - GFF2120 0.56 +0.2 8,202 - GFF2120 0.62 +0.4 8,267 + GFF2120 0.65 -0.8 8,293 - GFF2120 0.67 -0.6 8,312 + GFF2120 0.68 -1.1 8,322 - GFF2120 0.68 -0.8 8,442 - GFF2120 0.74 +0.1 8,445 + GFF2120 0.74 +0.7 8,552 + GFF2120 0.79 +0.3 8,626 + GFF2120 0.82 +0.0 8,626 + GFF2120 0.82 -0.5 8,626 + GFF2120 0.82 +0.3 8,868 + -0.3 8,869 - -1.3 8,916 + +1.1 8,916 + -0.7 8,927 - +1.2 9,057 - +0.1 9,117 + -1.3 9,299 + -0.2 9,303 + +0.4 9,303 + +0.5 9,303 + -0.2 9,304 - +0.1 9,348 + -0.8 9,349 - +0.2 9,350 + -2.2 9,653 - GFF2122 0.30 +0.1 9,702 - GFF2122 0.35 -0.2 9,716 + GFF2122 0.37 -0.2 9,717 - GFF2122 0.37 +0.8 9,767 + GFF2122 0.42 -0.3 9,897 - GFF2122 0.57 +0.0 9,900 + GFF2122 0.58 -0.9 9,900 + GFF2122 0.58 -0.4 9,936 + GFF2122 0.62 +0.1 9,937 - GFF2122 0.62 -0.4 9,937 - GFF2122 0.62 +0.1 9,941 + GFF2122 0.62 -0.3 9,941 + GFF2122 0.62 -0.3
Or see this region's nucleotide sequence