Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1970 and GFF1971 are separated by 341 nucleotides GFF1971 and GFF1972 are separated by 232 nucleotides GFF1972 and GFF1973 are separated by 203 nucleotides
GFF1970 - Uncharacterized PLP-dependent aminotransferase YfdZ, at 162,395 to 163,633
GFF1970
GFF1971 - hypothetical protein, at 163,975 to 164,121
GFF1971
GFF1972 - Glucokinase (EC 2.7.1.2), at 164,354 to 165,319
GFF1972
GFF1973 - Chloride channel protein, at 165,523 to 166,758
GFF1973
Position (kb) 164
165
166 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 163.363 kb on - strand, within GFF1970 at 163.363 kb on - strand, within GFF1970 at 163.363 kb on - strand, within GFF1970 at 163.375 kb on - strand, within GFF1970 at 163.523 kb on - strand at 163.571 kb on - strand at 163.983 kb on + strand at 163.983 kb on + strand at 164.081 kb on - strand, within GFF1971 at 164.083 kb on - strand, within GFF1971 at 164.358 kb on - strand at 164.369 kb on + strand at 164.369 kb on + strand at 164.414 kb on - strand at 164.452 kb on - strand, within GFF1972 at 164.452 kb on - strand, within GFF1972 at 164.542 kb on + strand, within GFF1972 at 164.544 kb on + strand, within GFF1972 at 164.544 kb on + strand, within GFF1972 at 164.824 kb on + strand, within GFF1972 at 164.825 kb on - strand, within GFF1972 at 165.132 kb on - strand, within GFF1972 at 165.290 kb on + strand at 165.291 kb on - strand at 165.303 kb on - strand at 165.303 kb on - strand at 165.309 kb on + strand at 165.310 kb on - strand at 165.416 kb on + strand at 165.419 kb on - strand at 165.493 kb on - strand at 165.493 kb on - strand at 165.498 kb on + strand at 165.498 kb on + strand at 165.522 kb on + strand at 165.523 kb on - strand at 165.698 kb on + strand, within GFF1973 at 165.705 kb on + strand, within GFF1973 at 165.706 kb on - strand, within GFF1973 at 165.842 kb on + strand, within GFF1973 at 165.867 kb on - strand, within GFF1973 at 165.900 kb on - strand, within GFF1973
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 163,363 - GFF1970 0.78 -1.6 163,363 - GFF1970 0.78 +0.3 163,363 - GFF1970 0.78 +0.1 163,375 - GFF1970 0.79 +0.3 163,523 - +0.0 163,571 - -0.7 163,983 + -0.2 163,983 + +0.1 164,081 - GFF1971 0.72 +0.3 164,083 - GFF1971 0.73 -0.3 164,358 - -0.8 164,369 + -0.1 164,369 + -0.3 164,414 - -2.9 164,452 - GFF1972 0.10 -0.9 164,452 - GFF1972 0.10 +0.2 164,542 + GFF1972 0.19 +0.9 164,544 + GFF1972 0.20 +0.1 164,544 + GFF1972 0.20 -0.8 164,824 + GFF1972 0.49 +0.2 164,825 - GFF1972 0.49 +0.3 165,132 - GFF1972 0.81 -0.1 165,290 + -0.6 165,291 - -0.1 165,303 - -0.6 165,303 - +1.5 165,309 + -0.8 165,310 - -0.6 165,416 + +0.6 165,419 - +0.7 165,493 - +2.3 165,493 - +1.2 165,498 + -1.0 165,498 + +0.0 165,522 + +0.3 165,523 - +0.3 165,698 + GFF1973 0.14 -0.9 165,705 + GFF1973 0.15 -1.0 165,706 - GFF1973 0.15 +0.2 165,842 + GFF1973 0.26 -0.3 165,867 - GFF1973 0.28 +0.5 165,900 - GFF1973 0.31 +0.2
Or see this region's nucleotide sequence