Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF174 and GFF175 are separated by 38 nucleotides GFF175 and GFF176 are separated by 118 nucleotides GFF176 and GFF177 are separated by 340 nucleotides
GFF174 - Thiamin ABC transporter, ATPase component / Thiamine transport ATP-binding protein thiQ, at 164,973 to 165,680
GFF174
GFF175 - DedA family inner membrane protein YabI, at 165,719 to 166,486
GFF175
GFF176 - Arabinose operon regulatory protein, at 166,605 to 167,450
GFF176
GFF177 - Ribulokinase (EC 2.7.1.16), at 167,791 to 169,500
GFF177
Position (kb) 166
167
168 Strain fitness (log2 ratio) -2
-1
0
1 at 165.753 kb on + strand at 165.754 kb on - strand at 165.754 kb on - strand at 165.755 kb on + strand at 165.829 kb on - strand, within GFF175 at 166.024 kb on - strand, within GFF175 at 166.160 kb on - strand, within GFF175 at 166.160 kb on - strand, within GFF175 at 166.443 kb on + strand at 166.462 kb on + strand at 166.463 kb on - strand at 166.486 kb on + strand at 166.486 kb on + strand at 166.487 kb on - strand at 166.487 kb on - strand at 166.487 kb on - strand at 166.487 kb on - strand at 166.487 kb on - strand at 166.563 kb on - strand at 166.563 kb on - strand at 166.566 kb on + strand at 166.567 kb on - strand at 166.573 kb on + strand at 166.573 kb on + strand at 166.606 kb on + strand at 166.788 kb on - strand, within GFF176 at 166.898 kb on - strand, within GFF176 at 166.898 kb on - strand, within GFF176 at 166.950 kb on - strand, within GFF176 at 166.961 kb on + strand, within GFF176 at 167.207 kb on - strand, within GFF176 at 167.238 kb on + strand, within GFF176 at 167.238 kb on + strand, within GFF176 at 167.316 kb on - strand, within GFF176 at 167.316 kb on - strand, within GFF176 at 167.554 kb on + strand at 167.631 kb on + strand at 167.633 kb on + strand at 167.633 kb on + strand at 167.882 kb on + strand at 167.883 kb on - strand at 167.960 kb on - strand at 168.006 kb on - strand, within GFF177 at 168.199 kb on - strand, within GFF177 at 168.242 kb on - strand, within GFF177
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 165,753 + -1.3 165,754 - -1.4 165,754 - -0.4 165,755 + -1.0 165,829 - GFF175 0.14 +0.3 166,024 - GFF175 0.40 -2.3 166,160 - GFF175 0.57 -1.2 166,160 - GFF175 0.57 -1.3 166,443 + -0.2 166,462 + -1.2 166,463 - -2.0 166,486 + -0.8 166,486 + -0.7 166,487 - -0.5 166,487 - -1.5 166,487 - -1.4 166,487 - +0.7 166,487 - -1.1 166,563 - +0.2 166,563 - +0.3 166,566 + +0.5 166,567 - +1.2 166,573 + +0.8 166,573 + -0.7 166,606 + +1.4 166,788 - GFF176 0.22 -0.0 166,898 - GFF176 0.35 -0.9 166,898 - GFF176 0.35 -0.2 166,950 - GFF176 0.41 -0.1 166,961 + GFF176 0.42 +0.7 167,207 - GFF176 0.71 -0.5 167,238 + GFF176 0.75 +0.6 167,238 + GFF176 0.75 +0.4 167,316 - GFF176 0.84 -0.1 167,316 - GFF176 0.84 +0.6 167,554 + +1.0 167,631 + +0.4 167,633 + -0.4 167,633 + +1.2 167,882 + -0.7 167,883 - +0.6 167,960 - +0.3 168,006 - GFF177 0.13 -1.0 168,199 - GFF177 0.24 -0.1 168,242 - GFF177 0.26 +0.2
Or see this region's nucleotide sequence