Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1634 and GFF1635 are separated by 116 nucleotides GFF1635 and GFF1636 are separated by 15 nucleotides
GFF1634 - Lipid A biosynthesis (KDO) 2-(lauroyl)-lipid IVA acyltransferase (EC 2.3.1.-), at 20,413 to 21,384
GFF1634
GFF1635 - Cell wall endopeptidase, family M23/M37, at 21,501 to 22,820
GFF1635
GFF1636 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 22,836 to 23,780
GFF1636
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 20.625 kb on + strand, within GFF1634 at 20.634 kb on - strand, within GFF1634 at 20.634 kb on - strand, within GFF1634 at 20.708 kb on + strand, within GFF1634 at 20.709 kb on - strand, within GFF1634 at 20.769 kb on - strand, within GFF1634 at 20.874 kb on - strand, within GFF1634 at 20.899 kb on - strand, within GFF1634 at 21.357 kb on - strand at 21.425 kb on - strand at 21.429 kb on + strand at 21.547 kb on + strand at 21.630 kb on + strand at 21.631 kb on - strand at 21.745 kb on + strand, within GFF1635 at 21.757 kb on + strand, within GFF1635 at 21.970 kb on - strand, within GFF1635 at 22.006 kb on + strand, within GFF1635 at 22.040 kb on - strand, within GFF1635 at 22.040 kb on - strand, within GFF1635 at 22.040 kb on - strand, within GFF1635 at 22.242 kb on - strand, within GFF1635 at 22.242 kb on - strand, within GFF1635 at 22.254 kb on - strand, within GFF1635 at 22.331 kb on - strand, within GFF1635 at 22.461 kb on - strand, within GFF1635 at 22.463 kb on + strand, within GFF1635 at 22.529 kb on - strand, within GFF1635 at 22.583 kb on + strand, within GFF1635 at 22.673 kb on + strand, within GFF1635 at 22.822 kb on + strand at 22.873 kb on - strand at 22.905 kb on - strand at 23.131 kb on + strand, within GFF1636 at 23.349 kb on - strand, within GFF1636 at 23.371 kb on - strand, within GFF1636 at 23.371 kb on - strand, within GFF1636 at 23.484 kb on - strand, within GFF1636 at 23.550 kb on + strand, within GFF1636 at 23.790 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 20,625 + GFF1634 0.22 -1.8 20,634 - GFF1634 0.23 -1.8 20,634 - GFF1634 0.23 -0.2 20,708 + GFF1634 0.30 -2.6 20,709 - GFF1634 0.30 -2.9 20,769 - GFF1634 0.37 -1.6 20,874 - GFF1634 0.47 -1.4 20,899 - GFF1634 0.50 -3.1 21,357 - -0.2 21,425 - -0.8 21,429 + +3.9 21,547 + -0.1 21,630 + +1.0 21,631 - +0.9 21,745 + GFF1635 0.18 +1.2 21,757 + GFF1635 0.19 +0.2 21,970 - GFF1635 0.36 +1.5 22,006 + GFF1635 0.38 +1.6 22,040 - GFF1635 0.41 +0.8 22,040 - GFF1635 0.41 +1.9 22,040 - GFF1635 0.41 +1.4 22,242 - GFF1635 0.56 +0.9 22,242 - GFF1635 0.56 +3.4 22,254 - GFF1635 0.57 +2.0 22,331 - GFF1635 0.63 +1.0 22,461 - GFF1635 0.73 +1.5 22,463 + GFF1635 0.73 +1.7 22,529 - GFF1635 0.78 +0.9 22,583 + GFF1635 0.82 +1.5 22,673 + GFF1635 0.89 +1.8 22,822 + +1.6 22,873 - +0.8 22,905 - -0.2 23,131 + GFF1636 0.31 +1.2 23,349 - GFF1636 0.54 +1.2 23,371 - GFF1636 0.57 -1.5 23,371 - GFF1636 0.57 +1.1 23,484 - GFF1636 0.69 -1.0 23,550 + GFF1636 0.76 +0.7 23,790 - +0.1
Or see this region's nucleotide sequence