Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1611 and GFF1612 are separated by 54 nucleotides GFF1612 and GFF1613 are separated by 64 nucleotides GFF1613 and GFF1614 are separated by 152 nucleotides GFF1614 and GFF1615 overlap by 16 nucleotides GFF1615 and GFF1616 are separated by 314 nucleotides
GFF1611 - Phage protein, at 933 to 1,196
GFF1611
GFF1612 - FIG01046582: hypothetical protein, at 1,251 to 1,439
GFF1612
GFF1613 - lytic enzyme, at 1,504 to 1,671
GFF1613
GFF1614 - hypothetical protein, at 1,824 to 1,943
GFF1614
GFF1615 - phage-tail assembly-like protein, at 1,928 to 2,461
GFF1615
GFF1616 - FIG01047716: hypothetical protein, at 2,776 to 3,429
GFF1616
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-1
0
1 at 0.931 kb on + strand at 0.935 kb on - strand at 1.061 kb on - strand, within GFF1611 at 1.398 kb on - strand, within GFF1612 at 1.557 kb on + strand, within GFF1613 at 1.612 kb on + strand, within GFF1613 at 1.612 kb on + strand, within GFF1613 at 1.613 kb on - strand, within GFF1613 at 1.710 kb on + strand at 1.711 kb on - strand at 1.946 kb on - strand at 1.971 kb on - strand at 2.282 kb on - strand, within GFF1615 at 2.517 kb on - strand at 2.546 kb on - strand at 2.682 kb on - strand at 2.773 kb on + strand at 2.773 kb on + strand at 2.774 kb on - strand at 2.850 kb on + strand, within GFF1616 at 3.087 kb on + strand, within GFF1616 at 3.166 kb on + strand, within GFF1616 at 3.272 kb on + strand, within GFF1616 at 3.273 kb on - strand, within GFF1616
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 931 + +1.0 935 - +0.3 1,061 - GFF1611 0.48 -0.6 1,398 - GFF1612 0.78 -0.9 1,557 + GFF1613 0.32 -0.1 1,612 + GFF1613 0.64 +0.0 1,612 + GFF1613 0.64 -1.0 1,613 - GFF1613 0.65 -0.0 1,710 + +0.5 1,711 - +0.1 1,946 - -0.2 1,971 - -0.7 2,282 - GFF1615 0.66 -0.8 2,517 - -0.7 2,546 - -0.4 2,682 - +0.9 2,773 + +0.9 2,773 + +1.0 2,774 - -0.4 2,850 + GFF1616 0.11 +0.6 3,087 + GFF1616 0.48 -0.4 3,166 + GFF1616 0.60 -0.0 3,272 + GFF1616 0.76 +0.2 3,273 - GFF1616 0.76 +0.2
Or see this region's nucleotide sequence