Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1586 and GFF1587 are separated by 354 nucleotides GFF1587 and GFF1588 overlap by 8 nucleotides
GFF1586 - Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1), at 42,819 to 43,778
GFF1586
GFF1587 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 44,133 to 45,335
GFF1587
GFF1588 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 45,328 to 46,392
GFF1588
Position (kb) 44
45
46 Strain fitness (log2 ratio) -1
0
1
2
3 at 43.139 kb on + strand, within GFF1586 at 43.289 kb on - strand, within GFF1586 at 43.507 kb on - strand, within GFF1586 at 43.537 kb on + strand, within GFF1586 at 43.584 kb on + strand, within GFF1586 at 43.595 kb on + strand, within GFF1586 at 43.729 kb on - strand at 44.265 kb on - strand, within GFF1587 at 44.300 kb on - strand, within GFF1587 at 44.405 kb on + strand, within GFF1587 at 44.492 kb on + strand, within GFF1587 at 44.911 kb on - strand, within GFF1587 at 44.939 kb on + strand, within GFF1587 at 44.966 kb on + strand, within GFF1587 at 45.067 kb on - strand, within GFF1587 at 45.067 kb on - strand, within GFF1587 at 45.267 kb on + strand at 45.267 kb on + strand at 45.290 kb on + strand at 45.290 kb on + strand at 45.291 kb on - strand at 45.291 kb on - strand at 45.323 kb on + strand at 45.373 kb on - strand at 45.601 kb on + strand, within GFF1588 at 45.769 kb on - strand, within GFF1588 at 45.983 kb on + strand, within GFF1588 at 45.984 kb on - strand, within GFF1588 at 45.995 kb on + strand, within GFF1588 at 45.996 kb on - strand, within GFF1588 at 45.996 kb on - strand, within GFF1588 at 46.037 kb on + strand, within GFF1588 at 46.098 kb on - strand, within GFF1588 at 46.208 kb on + strand, within GFF1588 at 46.209 kb on - strand, within GFF1588 at 46.211 kb on + strand, within GFF1588
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 43,139 + GFF1586 0.33 -0.3 43,289 - GFF1586 0.49 -0.4 43,507 - GFF1586 0.72 +0.7 43,537 + GFF1586 0.75 -0.4 43,584 + GFF1586 0.80 +0.2 43,595 + GFF1586 0.81 -0.6 43,729 - -0.8 44,265 - GFF1587 0.11 -0.2 44,300 - GFF1587 0.14 +0.5 44,405 + GFF1587 0.23 +0.0 44,492 + GFF1587 0.30 +0.4 44,911 - GFF1587 0.65 +0.4 44,939 + GFF1587 0.67 +0.6 44,966 + GFF1587 0.69 +0.7 45,067 - GFF1587 0.78 +0.4 45,067 - GFF1587 0.78 +0.5 45,267 + +0.9 45,267 + +0.1 45,290 + -0.2 45,290 + +0.5 45,291 - +0.4 45,291 - +1.0 45,323 + -1.0 45,373 - +0.6 45,601 + GFF1588 0.26 +0.5 45,769 - GFF1588 0.41 +1.1 45,983 + GFF1588 0.62 +2.7 45,984 - GFF1588 0.62 +0.5 45,995 + GFF1588 0.63 +0.3 45,996 - GFF1588 0.63 -0.2 45,996 - GFF1588 0.63 +1.0 46,037 + GFF1588 0.67 +3.0 46,098 - GFF1588 0.72 +2.6 46,208 + GFF1588 0.83 -0.3 46,209 - GFF1588 0.83 +0.2 46,211 + GFF1588 0.83 -0.1
Or see this region's nucleotide sequence