Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1096 and GFF1097 are separated by 91 nucleotides GFF1097 and GFF1098 are separated by 280 nucleotides
GFF1096 - Chitinase (EC 3.2.1.14), at 278,651 to 280,750
GFF1096
GFF1097 - Putative hydroxymethyltransferase, at 280,842 to 283,838
GFF1097
GFF1098 - FIG074102: hypothetical protein, at 284,119 to 284,823
GFF1098
Position (kb) 280
281
282
283
284 Strain fitness (log2 ratio) -2
-1
0
1
2 at 279.842 kb on + strand, within GFF1096 at 279.843 kb on - strand, within GFF1096 at 279.920 kb on + strand, within GFF1096 at 279.921 kb on - strand, within GFF1096 at 279.951 kb on + strand, within GFF1096 at 279.970 kb on + strand, within GFF1096 at 279.971 kb on - strand, within GFF1096 at 279.992 kb on + strand, within GFF1096 at 280.135 kb on - strand, within GFF1096 at 280.228 kb on - strand, within GFF1096 at 280.237 kb on + strand, within GFF1096 at 280.237 kb on + strand, within GFF1096 at 280.276 kb on + strand, within GFF1096 at 280.294 kb on + strand, within GFF1096 at 280.538 kb on - strand, within GFF1096 at 280.558 kb on + strand at 280.684 kb on + strand at 280.748 kb on + strand at 280.751 kb on + strand at 280.752 kb on - strand at 280.752 kb on - strand at 280.761 kb on + strand at 280.762 kb on - strand at 280.995 kb on - strand at 281.095 kb on + strand at 281.321 kb on + strand, within GFF1097 at 281.596 kb on + strand, within GFF1097 at 281.604 kb on + strand, within GFF1097 at 281.958 kb on + strand, within GFF1097 at 282.083 kb on - strand, within GFF1097 at 282.140 kb on + strand, within GFF1097 at 282.275 kb on - strand, within GFF1097 at 282.275 kb on - strand, within GFF1097 at 282.310 kb on - strand, within GFF1097 at 282.330 kb on + strand, within GFF1097 at 282.344 kb on - strand, within GFF1097 at 282.550 kb on - strand, within GFF1097 at 282.633 kb on + strand, within GFF1097 at 282.839 kb on - strand, within GFF1097 at 282.856 kb on + strand, within GFF1097 at 282.856 kb on + strand, within GFF1097 at 282.857 kb on - strand, within GFF1097 at 282.906 kb on + strand, within GFF1097 at 282.906 kb on + strand, within GFF1097 at 282.907 kb on - strand, within GFF1097 at 282.907 kb on - strand, within GFF1097 at 282.990 kb on + strand, within GFF1097 at 282.991 kb on - strand, within GFF1097 at 282.991 kb on - strand, within GFF1097 at 283.001 kb on - strand, within GFF1097 at 283.114 kb on - strand, within GFF1097 at 283.121 kb on + strand, within GFF1097 at 283.299 kb on + strand, within GFF1097 at 283.368 kb on + strand, within GFF1097 at 283.473 kb on - strand, within GFF1097 at 283.478 kb on + strand, within GFF1097 at 283.484 kb on - strand, within GFF1097 at 283.484 kb on - strand, within GFF1097 at 283.541 kb on + strand at 283.743 kb on - strand at 283.748 kb on + strand at 283.748 kb on + strand at 283.751 kb on + strand at 284.309 kb on - strand, within GFF1098 at 284.368 kb on - strand, within GFF1098 at 284.401 kb on - strand, within GFF1098 at 284.426 kb on - strand, within GFF1098 at 284.695 kb on - strand, within GFF1098
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 279,842 + GFF1096 0.57 +0.4 279,843 - GFF1096 0.57 +1.4 279,920 + GFF1096 0.60 -0.1 279,921 - GFF1096 0.60 -0.7 279,951 + GFF1096 0.62 +2.1 279,970 + GFF1096 0.63 -0.6 279,971 - GFF1096 0.63 +0.3 279,992 + GFF1096 0.64 +0.5 280,135 - GFF1096 0.71 +0.5 280,228 - GFF1096 0.75 +0.0 280,237 + GFF1096 0.76 +1.0 280,237 + GFF1096 0.76 -0.7 280,276 + GFF1096 0.77 -0.2 280,294 + GFF1096 0.78 +0.2 280,538 - GFF1096 0.90 +0.5 280,558 + -0.9 280,684 + -0.3 280,748 + +0.3 280,751 + -0.7 280,752 - -0.4 280,752 - +0.2 280,761 + +0.1 280,762 - -1.0 280,995 - -0.4 281,095 + +0.3 281,321 + GFF1097 0.16 +0.3 281,596 + GFF1097 0.25 +0.1 281,604 + GFF1097 0.25 -0.1 281,958 + GFF1097 0.37 +0.3 282,083 - GFF1097 0.41 -1.5 282,140 + GFF1097 0.43 +0.2 282,275 - GFF1097 0.48 -2.2 282,275 - GFF1097 0.48 +0.5 282,310 - GFF1097 0.49 +1.3 282,330 + GFF1097 0.50 -0.9 282,344 - GFF1097 0.50 +1.6 282,550 - GFF1097 0.57 -2.0 282,633 + GFF1097 0.60 +0.3 282,839 - GFF1097 0.67 +0.5 282,856 + GFF1097 0.67 -0.2 282,856 + GFF1097 0.67 -0.2 282,857 - GFF1097 0.67 -0.6 282,906 + GFF1097 0.69 +0.7 282,906 + GFF1097 0.69 -0.1 282,907 - GFF1097 0.69 -0.2 282,907 - GFF1097 0.69 -0.9 282,990 + GFF1097 0.72 +0.7 282,991 - GFF1097 0.72 +0.4 282,991 - GFF1097 0.72 +0.1 283,001 - GFF1097 0.72 -0.0 283,114 - GFF1097 0.76 +0.5 283,121 + GFF1097 0.76 +2.0 283,299 + GFF1097 0.82 +0.6 283,368 + GFF1097 0.84 -0.3 283,473 - GFF1097 0.88 +0.4 283,478 + GFF1097 0.88 +1.9 283,484 - GFF1097 0.88 +0.1 283,484 - GFF1097 0.88 -0.4 283,541 + -0.3 283,743 - -1.2 283,748 + +0.9 283,748 + -0.3 283,751 + -1.7 284,309 - GFF1098 0.27 -0.7 284,368 - GFF1098 0.35 +0.2 284,401 - GFF1098 0.40 +0.2 284,426 - GFF1098 0.44 -0.4 284,695 - GFF1098 0.82 +0.5
Or see this region's nucleotide sequence