Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3523 and EX28DRAFT_3524 are separated by 242 nucleotides EX28DRAFT_3524 and EX28DRAFT_3525 overlap by 1 nucleotides EX28DRAFT_3525 and EX28DRAFT_3526 are separated by 0 nucleotides EX28DRAFT_3526 and EX28DRAFT_3527 are separated by 51 nucleotides
EX28DRAFT_3523: EX28DRAFT_3523 - Predicted membrane protein, at 187,016 to 188,287
_3523
EX28DRAFT_3524: EX28DRAFT_3524 - Protein of unknown function (DUF2778), at 188,530 to 188,892
_3524
EX28DRAFT_3525: EX28DRAFT_3525 - hypothetical protein, at 188,892 to 189,404
_3525
EX28DRAFT_3526: EX28DRAFT_3526 - type VI secretion system effector, Hcp1 family, at 189,405 to 189,896
_3526
EX28DRAFT_3527: EX28DRAFT_3527 - Restriction endonuclease, at 189,948 to 191,130
_3527
Position (kb) 188
189
190 Strain fitness (log2 ratio) -2
-1
0
1 at 188.059 kb on + strand, within EX28DRAFT_3523 at 188.671 kb on - strand, within EX28DRAFT_3524 at 188.731 kb on - strand, within EX28DRAFT_3524 at 188.731 kb on - strand, within EX28DRAFT_3524 at 188.980 kb on + strand, within EX28DRAFT_3525 at 189.056 kb on + strand, within EX28DRAFT_3525 at 189.197 kb on - strand, within EX28DRAFT_3525 at 189.206 kb on - strand, within EX28DRAFT_3525 at 189.206 kb on - strand, within EX28DRAFT_3525 at 189.231 kb on - strand, within EX28DRAFT_3525 at 189.422 kb on + strand at 189.422 kb on + strand at 189.422 kb on + strand at 189.423 kb on - strand at 189.450 kb on - strand at 189.455 kb on - strand, within EX28DRAFT_3526 at 189.510 kb on + strand, within EX28DRAFT_3526 at 189.511 kb on - strand, within EX28DRAFT_3526 at 189.520 kb on + strand, within EX28DRAFT_3526 at 189.520 kb on + strand, within EX28DRAFT_3526 at 189.520 kb on + strand, within EX28DRAFT_3526 at 189.521 kb on - strand, within EX28DRAFT_3526 at 189.616 kb on - strand, within EX28DRAFT_3526 at 189.624 kb on - strand, within EX28DRAFT_3526 at 189.644 kb on + strand, within EX28DRAFT_3526 at 189.898 kb on + strand at 189.898 kb on + strand at 189.899 kb on - strand at 189.899 kb on - strand at 189.950 kb on - strand at 189.963 kb on + strand at 190.014 kb on + strand at 190.043 kb on + strand at 190.043 kb on + strand at 190.062 kb on + strand at 190.080 kb on + strand, within EX28DRAFT_3527 at 190.081 kb on - strand, within EX28DRAFT_3527 at 190.091 kb on + strand, within EX28DRAFT_3527 at 190.091 kb on + strand, within EX28DRAFT_3527 at 190.092 kb on - strand, within EX28DRAFT_3527 at 190.198 kb on - strand, within EX28DRAFT_3527 at 190.223 kb on + strand, within EX28DRAFT_3527 at 190.223 kb on + strand, within EX28DRAFT_3527 at 190.224 kb on - strand, within EX28DRAFT_3527 at 190.224 kb on - strand, within EX28DRAFT_3527 at 190.235 kb on + strand, within EX28DRAFT_3527 at 190.236 kb on - strand, within EX28DRAFT_3527 at 190.282 kb on - strand, within EX28DRAFT_3527 at 190.388 kb on - strand, within EX28DRAFT_3527
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 188,059 + EX28DRAFT_3523 0.82 -0.5 188,671 - EX28DRAFT_3524 0.39 -0.4 188,731 - EX28DRAFT_3524 0.55 +1.3 188,731 - EX28DRAFT_3524 0.55 +0.7 188,980 + EX28DRAFT_3525 0.17 -0.7 189,056 + EX28DRAFT_3525 0.32 -0.8 189,197 - EX28DRAFT_3525 0.59 +1.0 189,206 - EX28DRAFT_3525 0.61 -0.6 189,206 - EX28DRAFT_3525 0.61 -0.0 189,231 - EX28DRAFT_3525 0.66 -0.6 189,422 + -1.1 189,422 + +0.3 189,422 + +0.5 189,423 - -0.6 189,450 - +0.0 189,455 - EX28DRAFT_3526 0.10 -0.4 189,510 + EX28DRAFT_3526 0.21 +0.3 189,511 - EX28DRAFT_3526 0.22 -1.0 189,520 + EX28DRAFT_3526 0.23 +0.4 189,520 + EX28DRAFT_3526 0.23 +0.0 189,520 + EX28DRAFT_3526 0.23 -0.6 189,521 - EX28DRAFT_3526 0.24 -0.0 189,616 - EX28DRAFT_3526 0.43 -2.3 189,624 - EX28DRAFT_3526 0.45 -0.5 189,644 + EX28DRAFT_3526 0.49 +0.0 189,898 + -0.0 189,898 + +0.3 189,899 - -0.2 189,899 - -0.1 189,950 - -0.7 189,963 + +0.1 190,014 + +1.1 190,043 + -0.4 190,043 + -0.8 190,062 + -0.5 190,080 + EX28DRAFT_3527 0.11 -0.2 190,081 - EX28DRAFT_3527 0.11 +0.2 190,091 + EX28DRAFT_3527 0.12 +0.5 190,091 + EX28DRAFT_3527 0.12 -0.3 190,092 - EX28DRAFT_3527 0.12 -0.8 190,198 - EX28DRAFT_3527 0.21 -0.2 190,223 + EX28DRAFT_3527 0.23 +0.4 190,223 + EX28DRAFT_3527 0.23 +0.3 190,224 - EX28DRAFT_3527 0.23 +0.4 190,224 - EX28DRAFT_3527 0.23 -0.9 190,235 + EX28DRAFT_3527 0.24 +0.0 190,236 - EX28DRAFT_3527 0.24 -0.1 190,282 - EX28DRAFT_3527 0.28 -0.1 190,388 - EX28DRAFT_3527 0.37 +0.8
Or see this region's nucleotide sequence