Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3353 and EX28DRAFT_3354 are separated by 10 nucleotides EX28DRAFT_3354 and EX28DRAFT_3355 overlap by 8 nucleotides EX28DRAFT_3355 and EX28DRAFT_3356 are separated by 310 nucleotides
EX28DRAFT_3353: EX28DRAFT_3353 - Fumarate reductase subunit C, at 19,208 to 19,603
_3353
EX28DRAFT_3354: EX28DRAFT_3354 - succinate dehydrogenase and fumarate reductase iron-sulfur protein, at 19,614 to 20,348
_3354
EX28DRAFT_3355: EX28DRAFT_3355 - succinate dehydrogenase subunit A (EC 1.3.5.1), at 20,341 to 22,131
_3355
EX28DRAFT_3356: EX28DRAFT_3356 - EF-P lysine aminoacylase GenX, at 22,442 to 23,419
_3356
Position (kb) 20
21
22
23 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 19.471 kb on - strand, within EX28DRAFT_3353 at 19.528 kb on - strand, within EX28DRAFT_3353 at 19.581 kb on + strand at 19.581 kb on + strand at 19.582 kb on - strand at 19.582 kb on - strand at 19.582 kb on - strand at 19.582 kb on - strand at 19.615 kb on + strand at 19.772 kb on + strand, within EX28DRAFT_3354 at 19.772 kb on + strand, within EX28DRAFT_3354 at 19.772 kb on + strand, within EX28DRAFT_3354 at 19.773 kb on - strand, within EX28DRAFT_3354 at 19.990 kb on + strand, within EX28DRAFT_3354 at 19.991 kb on - strand, within EX28DRAFT_3354 at 19.998 kb on - strand, within EX28DRAFT_3354 at 20.082 kb on - strand, within EX28DRAFT_3354 at 20.095 kb on + strand, within EX28DRAFT_3354 at 20.114 kb on + strand, within EX28DRAFT_3354 at 20.115 kb on - strand, within EX28DRAFT_3354 at 20.189 kb on - strand, within EX28DRAFT_3354 at 20.269 kb on + strand, within EX28DRAFT_3354 at 20.270 kb on - strand, within EX28DRAFT_3354 at 20.309 kb on - strand at 20.401 kb on - strand at 20.401 kb on - strand at 20.436 kb on - strand at 20.436 kb on - strand at 20.577 kb on + strand, within EX28DRAFT_3355 at 20.577 kb on + strand, within EX28DRAFT_3355 at 20.578 kb on - strand, within EX28DRAFT_3355 at 20.578 kb on - strand, within EX28DRAFT_3355 at 20.618 kb on + strand, within EX28DRAFT_3355 at 20.727 kb on + strand, within EX28DRAFT_3355 at 20.733 kb on - strand, within EX28DRAFT_3355 at 20.736 kb on + strand, within EX28DRAFT_3355 at 20.950 kb on - strand, within EX28DRAFT_3355 at 21.015 kb on + strand, within EX28DRAFT_3355 at 21.019 kb on - strand, within EX28DRAFT_3355 at 21.191 kb on + strand, within EX28DRAFT_3355 at 21.192 kb on - strand, within EX28DRAFT_3355 at 21.287 kb on + strand, within EX28DRAFT_3355 at 21.287 kb on + strand, within EX28DRAFT_3355 at 21.288 kb on - strand, within EX28DRAFT_3355 at 21.438 kb on - strand, within EX28DRAFT_3355 at 21.526 kb on - strand, within EX28DRAFT_3355 at 21.528 kb on - strand, within EX28DRAFT_3355 at 21.606 kb on + strand, within EX28DRAFT_3355 at 21.607 kb on - strand, within EX28DRAFT_3355 at 21.607 kb on - strand, within EX28DRAFT_3355 at 21.879 kb on - strand, within EX28DRAFT_3355 at 21.879 kb on - strand, within EX28DRAFT_3355 at 21.879 kb on - strand, within EX28DRAFT_3355 at 22.078 kb on + strand at 22.079 kb on - strand at 22.079 kb on - strand at 22.173 kb on + strand at 22.174 kb on - strand at 22.178 kb on + strand at 22.179 kb on + strand at 22.180 kb on - strand at 22.235 kb on + strand at 22.236 kb on - strand at 22.243 kb on + strand at 22.243 kb on + strand at 22.244 kb on - strand at 22.246 kb on - strand at 22.254 kb on - strand at 22.308 kb on + strand at 22.328 kb on + strand at 22.328 kb on + strand at 22.328 kb on + strand at 22.329 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 19,471 - EX28DRAFT_3353 0.66 -0.4 19,528 - EX28DRAFT_3353 0.81 +0.4 19,581 + -1.2 19,581 + +0.5 19,582 - -0.6 19,582 - +0.6 19,582 - +0.1 19,582 - -0.3 19,615 + +0.4 19,772 + EX28DRAFT_3354 0.21 +0.1 19,772 + EX28DRAFT_3354 0.21 +0.5 19,772 + EX28DRAFT_3354 0.21 +0.3 19,773 - EX28DRAFT_3354 0.22 -1.1 19,990 + EX28DRAFT_3354 0.51 -0.0 19,991 - EX28DRAFT_3354 0.51 +0.4 19,998 - EX28DRAFT_3354 0.52 +0.4 20,082 - EX28DRAFT_3354 0.64 +0.0 20,095 + EX28DRAFT_3354 0.65 +0.6 20,114 + EX28DRAFT_3354 0.68 -2.3 20,115 - EX28DRAFT_3354 0.68 -1.9 20,189 - EX28DRAFT_3354 0.78 +0.3 20,269 + EX28DRAFT_3354 0.89 -0.4 20,270 - EX28DRAFT_3354 0.89 -0.3 20,309 - -0.2 20,401 - +0.2 20,401 - +0.0 20,436 - +0.3 20,436 - +0.1 20,577 + EX28DRAFT_3355 0.13 -0.8 20,577 + EX28DRAFT_3355 0.13 -0.4 20,578 - EX28DRAFT_3355 0.13 -0.7 20,578 - EX28DRAFT_3355 0.13 -0.6 20,618 + EX28DRAFT_3355 0.15 -0.1 20,727 + EX28DRAFT_3355 0.22 -0.9 20,733 - EX28DRAFT_3355 0.22 -0.4 20,736 + EX28DRAFT_3355 0.22 +0.1 20,950 - EX28DRAFT_3355 0.34 -0.9 21,015 + EX28DRAFT_3355 0.38 -0.4 21,019 - EX28DRAFT_3355 0.38 +0.7 21,191 + EX28DRAFT_3355 0.47 -0.6 21,192 - EX28DRAFT_3355 0.48 +0.8 21,287 + EX28DRAFT_3355 0.53 -0.1 21,287 + EX28DRAFT_3355 0.53 +0.9 21,288 - EX28DRAFT_3355 0.53 -0.0 21,438 - EX28DRAFT_3355 0.61 -0.4 21,526 - EX28DRAFT_3355 0.66 +3.9 21,528 - EX28DRAFT_3355 0.66 -0.5 21,606 + EX28DRAFT_3355 0.71 -0.4 21,607 - EX28DRAFT_3355 0.71 +0.5 21,607 - EX28DRAFT_3355 0.71 +0.3 21,879 - EX28DRAFT_3355 0.86 +0.1 21,879 - EX28DRAFT_3355 0.86 +0.2 21,879 - EX28DRAFT_3355 0.86 -0.5 22,078 + +0.1 22,079 - -0.6 22,079 - -0.2 22,173 + -2.3 22,174 - -0.3 22,178 + +0.0 22,179 + -0.7 22,180 - -0.2 22,235 + -0.3 22,236 - +0.4 22,243 + +0.5 22,243 + -0.9 22,244 - +0.2 22,246 - -0.4 22,254 - -0.8 22,308 + +0.1 22,328 + +0.0 22,328 + +0.0 22,328 + +0.3 22,329 - -0.1
Or see this region's nucleotide sequence