Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3162 and EX28DRAFT_3163 are separated by 344 nucleotides EX28DRAFT_3163 and EX28DRAFT_3164 are separated by 2 nucleotides EX28DRAFT_3164 and EX28DRAFT_3165 are separated by 142 nucleotides EX28DRAFT_3165 and EX28DRAFT_3166 are separated by 31 nucleotides
EX28DRAFT_3162: EX28DRAFT_3162 - Transcriptional regulators, at 466,052 to 466,828
_3162
EX28DRAFT_3163: EX28DRAFT_3163 - Uncharacterized membrane-associated protein, at 467,173 to 467,835
_3163
EX28DRAFT_3164: EX28DRAFT_3164 - SecD export protein N-terminal TM region, at 467,838 to 468,221
_3164
EX28DRAFT_3165: EX28DRAFT_3165 - Protein of unknown function (DUF1090), at 468,364 to 468,732
_3165
EX28DRAFT_3166: EX28DRAFT_3166 - Uncharacterized conserved protein, at 468,764 to 469,069
_3166
Position (kb) 467
468 Strain fitness (log2 ratio) -1
0
1
2 at 466.210 kb on + strand, within EX28DRAFT_3162 at 466.210 kb on + strand, within EX28DRAFT_3162 at 466.326 kb on - strand, within EX28DRAFT_3162 at 466.373 kb on - strand, within EX28DRAFT_3162 at 466.663 kb on + strand, within EX28DRAFT_3162 at 466.827 kb on - strand at 466.890 kb on + strand at 466.890 kb on + strand at 466.891 kb on - strand at 466.891 kb on - strand at 466.892 kb on + strand at 466.892 kb on + strand at 466.892 kb on + strand at 466.893 kb on - strand at 466.893 kb on - strand at 466.893 kb on - strand at 466.894 kb on + strand at 466.894 kb on + strand at 466.894 kb on + strand at 466.895 kb on - strand at 466.909 kb on + strand at 466.909 kb on + strand at 466.909 kb on + strand at 466.910 kb on - strand at 466.910 kb on - strand at 467.046 kb on + strand at 467.192 kb on + strand at 467.192 kb on + strand at 467.193 kb on - strand at 467.193 kb on - strand at 467.257 kb on + strand, within EX28DRAFT_3163 at 467.257 kb on + strand, within EX28DRAFT_3163 at 467.258 kb on - strand, within EX28DRAFT_3163 at 467.258 kb on - strand, within EX28DRAFT_3163 at 467.271 kb on + strand, within EX28DRAFT_3163 at 467.272 kb on - strand, within EX28DRAFT_3163 at 467.390 kb on + strand, within EX28DRAFT_3163 at 467.391 kb on - strand, within EX28DRAFT_3163 at 467.391 kb on - strand, within EX28DRAFT_3163 at 467.431 kb on + strand, within EX28DRAFT_3163 at 467.431 kb on + strand, within EX28DRAFT_3163 at 467.432 kb on - strand, within EX28DRAFT_3163 at 467.432 kb on - strand, within EX28DRAFT_3163 at 467.432 kb on - strand, within EX28DRAFT_3163 at 467.432 kb on - strand, within EX28DRAFT_3163 at 467.432 kb on - strand, within EX28DRAFT_3163 at 467.432 kb on - strand, within EX28DRAFT_3163 at 467.661 kb on - strand, within EX28DRAFT_3163 at 467.661 kb on - strand, within EX28DRAFT_3163 at 467.661 kb on - strand, within EX28DRAFT_3163 at 467.690 kb on - strand, within EX28DRAFT_3163 at 467.722 kb on + strand, within EX28DRAFT_3163 at 467.722 kb on + strand, within EX28DRAFT_3163 at 467.723 kb on - strand, within EX28DRAFT_3163 at 467.843 kb on + strand at 467.886 kb on - strand, within EX28DRAFT_3164 at 467.984 kb on - strand, within EX28DRAFT_3164 at 467.984 kb on - strand, within EX28DRAFT_3164 at 468.065 kb on - strand, within EX28DRAFT_3164 at 468.138 kb on + strand, within EX28DRAFT_3164 at 468.139 kb on - strand, within EX28DRAFT_3164 at 468.140 kb on + strand, within EX28DRAFT_3164 at 468.140 kb on + strand, within EX28DRAFT_3164 at 468.141 kb on - strand, within EX28DRAFT_3164 at 468.141 kb on - strand, within EX28DRAFT_3164 at 468.220 kb on - strand at 468.269 kb on + strand at 468.269 kb on + strand at 468.270 kb on - strand at 468.273 kb on + strand at 468.273 kb on + strand at 468.274 kb on - strand at 468.274 kb on - strand at 468.276 kb on + strand at 468.276 kb on + strand at 468.277 kb on - strand at 468.282 kb on + strand at 468.282 kb on + strand at 468.282 kb on + strand at 468.282 kb on + strand at 468.283 kb on - strand at 468.283 kb on - strand at 468.404 kb on + strand, within EX28DRAFT_3165 at 468.472 kb on + strand, within EX28DRAFT_3165 at 468.486 kb on + strand, within EX28DRAFT_3165 at 468.513 kb on + strand, within EX28DRAFT_3165
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 466,210 + EX28DRAFT_3162 0.20 -0.8 466,210 + EX28DRAFT_3162 0.20 -0.6 466,326 - EX28DRAFT_3162 0.35 +0.3 466,373 - EX28DRAFT_3162 0.41 -0.5 466,663 + EX28DRAFT_3162 0.79 -0.0 466,827 - -0.1 466,890 + +0.1 466,890 + -0.0 466,891 - +0.5 466,891 - -0.0 466,892 + +0.3 466,892 + -0.3 466,892 + +1.6 466,893 - -0.7 466,893 - -0.3 466,893 - -0.0 466,894 + +0.6 466,894 + +0.4 466,894 + +0.3 466,895 - -0.0 466,909 + +0.1 466,909 + -0.1 466,909 + +0.4 466,910 - -0.2 466,910 - -0.1 467,046 + +0.5 467,192 + -0.2 467,192 + -0.2 467,193 - -1.6 467,193 - +0.3 467,257 + EX28DRAFT_3163 0.13 -0.4 467,257 + EX28DRAFT_3163 0.13 -0.1 467,258 - EX28DRAFT_3163 0.13 -0.5 467,258 - EX28DRAFT_3163 0.13 -0.1 467,271 + EX28DRAFT_3163 0.15 +0.1 467,272 - EX28DRAFT_3163 0.15 -0.5 467,390 + EX28DRAFT_3163 0.33 -0.0 467,391 - EX28DRAFT_3163 0.33 -0.2 467,391 - EX28DRAFT_3163 0.33 -1.1 467,431 + EX28DRAFT_3163 0.39 +0.3 467,431 + EX28DRAFT_3163 0.39 +0.9 467,432 - EX28DRAFT_3163 0.39 +1.0 467,432 - EX28DRAFT_3163 0.39 -0.6 467,432 - EX28DRAFT_3163 0.39 -0.8 467,432 - EX28DRAFT_3163 0.39 -0.4 467,432 - EX28DRAFT_3163 0.39 +1.2 467,432 - EX28DRAFT_3163 0.39 +0.1 467,661 - EX28DRAFT_3163 0.74 -0.4 467,661 - EX28DRAFT_3163 0.74 +0.2 467,661 - EX28DRAFT_3163 0.74 +1.0 467,690 - EX28DRAFT_3163 0.78 +0.2 467,722 + EX28DRAFT_3163 0.83 -0.4 467,722 + EX28DRAFT_3163 0.83 -1.1 467,723 - EX28DRAFT_3163 0.83 -0.6 467,843 + +0.5 467,886 - EX28DRAFT_3164 0.12 +0.6 467,984 - EX28DRAFT_3164 0.38 +0.2 467,984 - EX28DRAFT_3164 0.38 -0.2 468,065 - EX28DRAFT_3164 0.59 -0.2 468,138 + EX28DRAFT_3164 0.78 -0.3 468,139 - EX28DRAFT_3164 0.78 +0.3 468,140 + EX28DRAFT_3164 0.79 +0.3 468,140 + EX28DRAFT_3164 0.79 +0.0 468,141 - EX28DRAFT_3164 0.79 -0.3 468,141 - EX28DRAFT_3164 0.79 -0.3 468,220 - -0.0 468,269 + -0.0 468,269 + -0.0 468,270 - +0.0 468,273 + -0.2 468,273 + +1.2 468,274 - +0.2 468,274 - -0.6 468,276 + +0.2 468,276 + +0.1 468,277 - -0.5 468,282 + -0.9 468,282 + +0.9 468,282 + +2.2 468,282 + +0.1 468,283 - +0.4 468,283 - +0.7 468,404 + EX28DRAFT_3165 0.11 -0.1 468,472 + EX28DRAFT_3165 0.29 -0.1 468,486 + EX28DRAFT_3165 0.33 -0.7 468,513 + EX28DRAFT_3165 0.40 -1.0
Or see this region's nucleotide sequence