Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2855 and EX28DRAFT_2856 are separated by 54 nucleotides EX28DRAFT_2856 and EX28DRAFT_2857 overlap by 1 nucleotides EX28DRAFT_2857 and EX28DRAFT_2858 are separated by 165 nucleotides EX28DRAFT_2858 and EX28DRAFT_2859 overlap by 44 nucleotides
EX28DRAFT_2855: EX28DRAFT_2855 - putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE, at 157,230 to 157,901
_2855
EX28DRAFT_2856: EX28DRAFT_2856 - Putative N-acetylmannosamine-6-phosphate epimerase, at 157,956 to 158,663
_2856
EX28DRAFT_2857: EX28DRAFT_2857 - PTS system, glucose-like IIB component, at 158,663 to 160,222
_2857
EX28DRAFT_2858: EX28DRAFT_2858 - transcriptional regulator, RpiR family, at 160,388 to 161,212
_2858
EX28DRAFT_2859: EX28DRAFT_2859 - hypothetical protein, at 161,169 to 161,351
_2859
Position (kb) 158
159
160
161 Strain fitness (log2 ratio) -2
-1
0
1
2 at 157.731 kb on + strand, within EX28DRAFT_2855 at 157.737 kb on - strand, within EX28DRAFT_2855 at 157.737 kb on - strand, within EX28DRAFT_2855 at 157.737 kb on - strand, within EX28DRAFT_2855 at 157.775 kb on + strand, within EX28DRAFT_2855 at 157.775 kb on + strand, within EX28DRAFT_2855 at 157.775 kb on + strand, within EX28DRAFT_2855 at 157.776 kb on - strand, within EX28DRAFT_2855 at 157.776 kb on - strand, within EX28DRAFT_2855 at 157.818 kb on + strand, within EX28DRAFT_2855 at 157.852 kb on + strand at 157.852 kb on + strand at 157.852 kb on + strand at 157.902 kb on - strand at 157.931 kb on - strand at 157.934 kb on - strand at 157.994 kb on + strand at 157.995 kb on - strand at 158.007 kb on - strand at 158.049 kb on - strand, within EX28DRAFT_2856 at 158.195 kb on + strand, within EX28DRAFT_2856 at 158.196 kb on - strand, within EX28DRAFT_2856 at 158.196 kb on - strand, within EX28DRAFT_2856 at 158.225 kb on + strand, within EX28DRAFT_2856 at 158.226 kb on - strand, within EX28DRAFT_2856 at 158.270 kb on + strand, within EX28DRAFT_2856 at 158.450 kb on - strand, within EX28DRAFT_2856 at 158.496 kb on - strand, within EX28DRAFT_2856 at 158.576 kb on + strand, within EX28DRAFT_2856 at 158.576 kb on + strand, within EX28DRAFT_2856 at 158.576 kb on + strand, within EX28DRAFT_2856 at 158.577 kb on - strand, within EX28DRAFT_2856 at 158.664 kb on + strand at 158.715 kb on + strand at 158.715 kb on + strand at 158.716 kb on - strand at 158.716 kb on - strand at 159.115 kb on + strand, within EX28DRAFT_2857 at 159.116 kb on - strand, within EX28DRAFT_2857 at 159.171 kb on + strand, within EX28DRAFT_2857 at 159.171 kb on + strand, within EX28DRAFT_2857 at 159.171 kb on + strand, within EX28DRAFT_2857 at 159.172 kb on - strand, within EX28DRAFT_2857 at 159.301 kb on - strand, within EX28DRAFT_2857 at 159.301 kb on - strand, within EX28DRAFT_2857 at 159.301 kb on - strand, within EX28DRAFT_2857 at 159.341 kb on - strand, within EX28DRAFT_2857 at 159.343 kb on + strand, within EX28DRAFT_2857 at 159.379 kb on - strand, within EX28DRAFT_2857 at 159.379 kb on - strand, within EX28DRAFT_2857 at 159.496 kb on - strand, within EX28DRAFT_2857 at 159.496 kb on - strand, within EX28DRAFT_2857 at 159.496 kb on - strand, within EX28DRAFT_2857 at 159.546 kb on + strand, within EX28DRAFT_2857 at 159.672 kb on - strand, within EX28DRAFT_2857 at 160.103 kb on + strand at 160.357 kb on + strand at 160.358 kb on - strand at 160.377 kb on - strand at 160.500 kb on - strand, within EX28DRAFT_2858 at 160.587 kb on - strand, within EX28DRAFT_2858 at 160.775 kb on + strand, within EX28DRAFT_2858 at 160.817 kb on + strand, within EX28DRAFT_2858 at 161.073 kb on + strand, within EX28DRAFT_2858 at 161.073 kb on + strand, within EX28DRAFT_2858 at 161.095 kb on + strand, within EX28DRAFT_2858
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 157,731 + EX28DRAFT_2855 0.75 +0.2 157,737 - EX28DRAFT_2855 0.75 -0.1 157,737 - EX28DRAFT_2855 0.75 -0.9 157,737 - EX28DRAFT_2855 0.75 +0.1 157,775 + EX28DRAFT_2855 0.81 +0.3 157,775 + EX28DRAFT_2855 0.81 +0.1 157,775 + EX28DRAFT_2855 0.81 -0.2 157,776 - EX28DRAFT_2855 0.81 -0.0 157,776 - EX28DRAFT_2855 0.81 +2.1 157,818 + EX28DRAFT_2855 0.88 -0.3 157,852 + -0.5 157,852 + +0.5 157,852 + +0.9 157,902 - +0.6 157,931 - -0.2 157,934 - +0.3 157,994 + -0.7 157,995 - -0.3 158,007 - -0.0 158,049 - EX28DRAFT_2856 0.13 -0.1 158,195 + EX28DRAFT_2856 0.34 -0.0 158,196 - EX28DRAFT_2856 0.34 -0.2 158,196 - EX28DRAFT_2856 0.34 +0.2 158,225 + EX28DRAFT_2856 0.38 -0.1 158,226 - EX28DRAFT_2856 0.38 -0.1 158,270 + EX28DRAFT_2856 0.44 -0.0 158,450 - EX28DRAFT_2856 0.70 +0.1 158,496 - EX28DRAFT_2856 0.76 +0.5 158,576 + EX28DRAFT_2856 0.88 +0.2 158,576 + EX28DRAFT_2856 0.88 +1.2 158,576 + EX28DRAFT_2856 0.88 -1.2 158,577 - EX28DRAFT_2856 0.88 -0.4 158,664 + +0.1 158,715 + -0.0 158,715 + +1.6 158,716 - -0.1 158,716 - -0.1 159,115 + EX28DRAFT_2857 0.29 -0.3 159,116 - EX28DRAFT_2857 0.29 +0.2 159,171 + EX28DRAFT_2857 0.33 -0.2 159,171 + EX28DRAFT_2857 0.33 +1.4 159,171 + EX28DRAFT_2857 0.33 +0.2 159,172 - EX28DRAFT_2857 0.33 +0.7 159,301 - EX28DRAFT_2857 0.41 +0.2 159,301 - EX28DRAFT_2857 0.41 -0.3 159,301 - EX28DRAFT_2857 0.41 +1.1 159,341 - EX28DRAFT_2857 0.43 +0.2 159,343 + EX28DRAFT_2857 0.44 +0.2 159,379 - EX28DRAFT_2857 0.46 +0.3 159,379 - EX28DRAFT_2857 0.46 +0.5 159,496 - EX28DRAFT_2857 0.53 +0.6 159,496 - EX28DRAFT_2857 0.53 +0.6 159,496 - EX28DRAFT_2857 0.53 -0.4 159,546 + EX28DRAFT_2857 0.57 +0.2 159,672 - EX28DRAFT_2857 0.65 -0.5 160,103 + -1.6 160,357 + +0.4 160,358 - -0.1 160,377 - -0.1 160,500 - EX28DRAFT_2858 0.14 -0.1 160,587 - EX28DRAFT_2858 0.24 -0.1 160,775 + EX28DRAFT_2858 0.47 -1.8 160,817 + EX28DRAFT_2858 0.52 +0.2 161,073 + EX28DRAFT_2858 0.83 +0.1 161,073 + EX28DRAFT_2858 0.83 +0.1 161,095 + EX28DRAFT_2858 0.86 +0.0
Or see this region's nucleotide sequence