Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1795 and EX28DRAFT_1796 are separated by 9 nucleotides EX28DRAFT_1796 and EX28DRAFT_1797 are separated by 40 nucleotides EX28DRAFT_1797 and EX28DRAFT_1798 are separated by 26 nucleotides EX28DRAFT_1798 and EX28DRAFT_1799 are separated by 19 nucleotides
EX28DRAFT_1795: EX28DRAFT_1795 - pseudouridine synthase, at 1,809,502 to 1,810,155
_1795
EX28DRAFT_1796: EX28DRAFT_1796 - ADP-ribose pyrophosphatase, at 1,810,165 to 1,810,638
_1796
EX28DRAFT_1797: EX28DRAFT_1797 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 1,810,679 to 1,811,791
_1797
EX28DRAFT_1798: EX28DRAFT_1798 - Uncharacterized protein involved in purine metabolism, at 1,811,818 to 1,812,447
_1798
EX28DRAFT_1799: EX28DRAFT_1799 - adenylosuccinate lyase, at 1,812,467 to 1,813,837
_1799
Position (kb) 1810
1811
1812 Strain fitness (log2 ratio) -1
0
1 at 1809.697 kb on + strand, within EX28DRAFT_1795 at 1809.698 kb on - strand, within EX28DRAFT_1795 at 1809.698 kb on - strand, within EX28DRAFT_1795 at 1809.699 kb on + strand, within EX28DRAFT_1795 at 1809.700 kb on - strand, within EX28DRAFT_1795 at 1809.826 kb on + strand, within EX28DRAFT_1795 at 1809.827 kb on - strand, within EX28DRAFT_1795 at 1809.833 kb on + strand, within EX28DRAFT_1795 at 1810.164 kb on + strand at 1810.165 kb on - strand at 1810.284 kb on + strand, within EX28DRAFT_1796 at 1810.285 kb on - strand, within EX28DRAFT_1796 at 1810.596 kb on + strand at 1811.805 kb on + strand at 1811.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,809,697 + EX28DRAFT_1795 0.30 -0.1 1,809,698 - EX28DRAFT_1795 0.30 +0.3 1,809,698 - EX28DRAFT_1795 0.30 +0.2 1,809,699 + EX28DRAFT_1795 0.30 -1.3 1,809,700 - EX28DRAFT_1795 0.30 +0.2 1,809,826 + EX28DRAFT_1795 0.50 -0.8 1,809,827 - EX28DRAFT_1795 0.50 +0.1 1,809,833 + EX28DRAFT_1795 0.51 +0.7 1,810,164 + +1.3 1,810,165 - -0.3 1,810,284 + EX28DRAFT_1796 0.25 +0.3 1,810,285 - EX28DRAFT_1796 0.25 +0.6 1,810,596 + -0.7 1,811,805 + -0.1 1,811,833 + -0.5
Or see this region's nucleotide sequence