Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1649 and EX28DRAFT_1650 are separated by 95 nucleotides EX28DRAFT_1650 and EX28DRAFT_1651 are separated by 44 nucleotides EX28DRAFT_1651 and EX28DRAFT_1652 are separated by 338 nucleotides
EX28DRAFT_1649: EX28DRAFT_1649 - lactoylglutathione lyase, at 1,666,007 to 1,666,414
_1649
EX28DRAFT_1650: EX28DRAFT_1650 - ribonuclease T, at 1,666,510 to 1,667,160
_1650
EX28DRAFT_1651: EX28DRAFT_1651 - monothiol glutaredoxin, Grx4 family, at 1,667,205 to 1,667,552
_1651
EX28DRAFT_1652: EX28DRAFT_1652 - Cell wall-associated hydrolases (invasion-associated proteins), at 1,667,891 to 1,668,664
_1652
Position (kb)
1667
1668 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1666.315 kb on - strand, within EX28DRAFT_1649 at 1666.440 kb on + strand at 1666.498 kb on + strand at 1666.786 kb on + strand, within EX28DRAFT_1650 at 1667.004 kb on + strand, within EX28DRAFT_1650 at 1667.191 kb on - strand at 1667.206 kb on + strand at 1667.282 kb on - strand, within EX28DRAFT_1651 at 1667.360 kb on - strand, within EX28DRAFT_1651 at 1667.360 kb on - strand, within EX28DRAFT_1651 at 1667.400 kb on - strand, within EX28DRAFT_1651 at 1667.492 kb on + strand, within EX28DRAFT_1651 at 1667.493 kb on - strand, within EX28DRAFT_1651 at 1667.494 kb on + strand, within EX28DRAFT_1651 at 1667.494 kb on + strand, within EX28DRAFT_1651 at 1667.495 kb on - strand, within EX28DRAFT_1651 at 1667.495 kb on - strand, within EX28DRAFT_1651 at 1667.567 kb on - strand at 1667.567 kb on - strand at 1667.567 kb on - strand at 1667.567 kb on - strand at 1667.641 kb on + strand at 1667.641 kb on + strand at 1667.643 kb on + strand at 1667.644 kb on - strand at 1667.650 kb on + strand at 1667.688 kb on + strand at 1667.780 kb on + strand at 1667.781 kb on - strand at 1667.781 kb on - strand at 1667.781 kb on - strand at 1667.781 kb on - strand at 1667.796 kb on - strand at 1667.798 kb on + strand at 1667.814 kb on + strand at 1667.821 kb on + strand at 1667.822 kb on - strand at 1667.822 kb on - strand at 1667.839 kb on + strand at 1667.839 kb on + strand at 1667.839 kb on + strand at 1667.931 kb on + strand at 1667.938 kb on + strand at 1668.247 kb on + strand, within EX28DRAFT_1652 at 1668.314 kb on + strand, within EX28DRAFT_1652 at 1668.371 kb on + strand, within EX28DRAFT_1652 at 1668.371 kb on + strand, within EX28DRAFT_1652 at 1668.378 kb on - strand, within EX28DRAFT_1652 at 1668.379 kb on + strand, within EX28DRAFT_1652 at 1668.425 kb on + strand, within EX28DRAFT_1652 at 1668.425 kb on + strand, within EX28DRAFT_1652 at 1668.530 kb on + strand, within EX28DRAFT_1652 at 1668.531 kb on - strand, within EX28DRAFT_1652
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,666,315 - EX28DRAFT_1649 0.75 -0.0 1,666,440 + +0.9 1,666,498 + +0.4 1,666,786 + EX28DRAFT_1650 0.42 -2.0 1,667,004 + EX28DRAFT_1650 0.76 -1.1 1,667,191 - -0.9 1,667,206 + +0.0 1,667,282 - EX28DRAFT_1651 0.22 -0.8 1,667,360 - EX28DRAFT_1651 0.45 -0.9 1,667,360 - EX28DRAFT_1651 0.45 +1.2 1,667,400 - EX28DRAFT_1651 0.56 +0.1 1,667,492 + EX28DRAFT_1651 0.82 -3.7 1,667,493 - EX28DRAFT_1651 0.83 -1.1 1,667,494 + EX28DRAFT_1651 0.83 -1.0 1,667,494 + EX28DRAFT_1651 0.83 -0.9 1,667,495 - EX28DRAFT_1651 0.83 -2.4 1,667,495 - EX28DRAFT_1651 0.83 -0.1 1,667,567 - +1.2 1,667,567 - +0.1 1,667,567 - -0.8 1,667,567 - -0.5 1,667,641 + -0.3 1,667,641 + +0.0 1,667,643 + +0.2 1,667,644 - -0.5 1,667,650 + -0.0 1,667,688 + +0.7 1,667,780 + -0.7 1,667,781 - +0.1 1,667,781 - +0.6 1,667,781 - +0.2 1,667,781 - -0.4 1,667,796 - +0.7 1,667,798 + +0.3 1,667,814 + -0.8 1,667,821 + +1.2 1,667,822 - +0.4 1,667,822 - -0.2 1,667,839 + -1.3 1,667,839 + +0.3 1,667,839 + +0.1 1,667,931 + -0.9 1,667,938 + -0.0 1,668,247 + EX28DRAFT_1652 0.46 -0.5 1,668,314 + EX28DRAFT_1652 0.55 -2.9 1,668,371 + EX28DRAFT_1652 0.62 +0.4 1,668,371 + EX28DRAFT_1652 0.62 +0.4 1,668,378 - EX28DRAFT_1652 0.63 +0.0 1,668,379 + EX28DRAFT_1652 0.63 -0.7 1,668,425 + EX28DRAFT_1652 0.69 +0.6 1,668,425 + EX28DRAFT_1652 0.69 +0.1 1,668,530 + EX28DRAFT_1652 0.83 +0.6 1,668,531 - EX28DRAFT_1652 0.83 -0.2
Or see this region's nucleotide sequence