Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1329 and EX28DRAFT_1330 are separated by 117 nucleotides EX28DRAFT_1330 and EX28DRAFT_1331 are separated by 20 nucleotides EX28DRAFT_1331 and EX28DRAFT_1332 are separated by 180 nucleotides EX28DRAFT_1332 and EX28DRAFT_1333 are separated by 62 nucleotides
EX28DRAFT_1329: EX28DRAFT_1329 - Site-specific recombinase XerD, at 1,325,096 to 1,326,376
_1329
EX28DRAFT_1330: EX28DRAFT_1330 - hypothetical protein, at 1,326,494 to 1,326,688
_1330
EX28DRAFT_1331: EX28DRAFT_1331 - hypothetical protein, at 1,326,709 to 1,327,116
_1331
EX28DRAFT_1332: EX28DRAFT_1332 - Phage minor tail protein, at 1,327,297 to 1,327,511
_1332
EX28DRAFT_1333: EX28DRAFT_1333 - hypothetical protein, at 1,327,574 to 1,327,998
_1333
Position (kb)
1326
1327 Strain fitness (log2 ratio)
-2
-1
0
1 at 1325.910 kb on + strand, within EX28DRAFT_1329 at 1325.911 kb on - strand, within EX28DRAFT_1329 at 1325.954 kb on + strand, within EX28DRAFT_1329 at 1325.954 kb on + strand, within EX28DRAFT_1329 at 1325.954 kb on + strand, within EX28DRAFT_1329 at 1325.955 kb on - strand, within EX28DRAFT_1329 at 1325.955 kb on - strand, within EX28DRAFT_1329 at 1325.955 kb on - strand, within EX28DRAFT_1329 at 1326.081 kb on - strand, within EX28DRAFT_1329 at 1326.173 kb on + strand, within EX28DRAFT_1329 at 1326.173 kb on + strand, within EX28DRAFT_1329 at 1326.174 kb on - strand, within EX28DRAFT_1329 at 1326.174 kb on - strand, within EX28DRAFT_1329 at 1326.309 kb on + strand at 1326.441 kb on - strand at 1326.441 kb on - strand at 1326.441 kb on - strand at 1326.452 kb on - strand at 1326.458 kb on + strand at 1326.463 kb on - strand at 1326.463 kb on - strand at 1326.477 kb on + strand at 1326.497 kb on + strand at 1326.497 kb on + strand at 1326.497 kb on + strand at 1326.497 kb on + strand at 1326.498 kb on - strand at 1326.498 kb on - strand at 1326.539 kb on - strand, within EX28DRAFT_1330 at 1326.564 kb on + strand, within EX28DRAFT_1330 at 1326.651 kb on - strand, within EX28DRAFT_1330 at 1326.694 kb on - strand at 1326.695 kb on + strand at 1326.707 kb on + strand at 1326.765 kb on - strand, within EX28DRAFT_1331 at 1326.910 kb on - strand, within EX28DRAFT_1331 at 1326.916 kb on - strand, within EX28DRAFT_1331 at 1326.936 kb on + strand, within EX28DRAFT_1331 at 1326.974 kb on - strand, within EX28DRAFT_1331 at 1327.022 kb on + strand, within EX28DRAFT_1331 at 1327.024 kb on + strand, within EX28DRAFT_1331 at 1327.025 kb on - strand, within EX28DRAFT_1331 at 1327.083 kb on + strand at 1327.137 kb on + strand at 1327.151 kb on + strand at 1327.211 kb on + strand at 1327.212 kb on - strand at 1327.213 kb on + strand at 1327.214 kb on - strand at 1327.274 kb on + strand at 1327.275 kb on - strand at 1327.281 kb on + strand at 1327.282 kb on - strand at 1327.443 kb on + strand, within EX28DRAFT_1332 at 1327.444 kb on - strand, within EX28DRAFT_1332 at 1327.447 kb on + strand, within EX28DRAFT_1332 at 1327.451 kb on + strand, within EX28DRAFT_1332 at 1327.494 kb on - strand at 1327.597 kb on + strand at 1327.615 kb on + strand at 1327.615 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,325,910 + EX28DRAFT_1329 0.64 -0.5 1,325,911 - EX28DRAFT_1329 0.64 -0.4 1,325,954 + EX28DRAFT_1329 0.67 -1.4 1,325,954 + EX28DRAFT_1329 0.67 +0.1 1,325,954 + EX28DRAFT_1329 0.67 -1.0 1,325,955 - EX28DRAFT_1329 0.67 -0.4 1,325,955 - EX28DRAFT_1329 0.67 +0.5 1,325,955 - EX28DRAFT_1329 0.67 +0.6 1,326,081 - EX28DRAFT_1329 0.77 -0.3 1,326,173 + EX28DRAFT_1329 0.84 +0.8 1,326,173 + EX28DRAFT_1329 0.84 +0.3 1,326,174 - EX28DRAFT_1329 0.84 +0.0 1,326,174 - EX28DRAFT_1329 0.84 +0.1 1,326,309 + +0.4 1,326,441 - -0.4 1,326,441 - +0.9 1,326,441 - -0.8 1,326,452 - +0.1 1,326,458 + -0.6 1,326,463 - -0.2 1,326,463 - +0.2 1,326,477 + -0.5 1,326,497 + -1.8 1,326,497 + -0.6 1,326,497 + +0.4 1,326,497 + +1.0 1,326,498 - -0.4 1,326,498 - +0.7 1,326,539 - EX28DRAFT_1330 0.23 -0.6 1,326,564 + EX28DRAFT_1330 0.36 -1.1 1,326,651 - EX28DRAFT_1330 0.81 -0.1 1,326,694 - -0.2 1,326,695 + +1.0 1,326,707 + +0.4 1,326,765 - EX28DRAFT_1331 0.14 -0.6 1,326,910 - EX28DRAFT_1331 0.49 -1.9 1,326,916 - EX28DRAFT_1331 0.51 -0.3 1,326,936 + EX28DRAFT_1331 0.56 +0.4 1,326,974 - EX28DRAFT_1331 0.65 +0.0 1,327,022 + EX28DRAFT_1331 0.77 -0.5 1,327,024 + EX28DRAFT_1331 0.77 +0.1 1,327,025 - EX28DRAFT_1331 0.77 +0.4 1,327,083 + -1.3 1,327,137 + +0.4 1,327,151 + -1.1 1,327,211 + -0.2 1,327,212 - +0.9 1,327,213 + +0.2 1,327,214 - +0.4 1,327,274 + +0.5 1,327,275 - +1.4 1,327,281 + -0.6 1,327,282 - +0.3 1,327,443 + EX28DRAFT_1332 0.68 -0.2 1,327,444 - EX28DRAFT_1332 0.68 -0.2 1,327,447 + EX28DRAFT_1332 0.70 -0.5 1,327,451 + EX28DRAFT_1332 0.72 +0.5 1,327,494 - -1.4 1,327,597 + +0.6 1,327,615 + +0.4 1,327,615 + -0.3
Or see this region's nucleotide sequence