Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0087 and EX28DRAFT_0088 are separated by 3 nucleotides EX28DRAFT_0088 and EX28DRAFT_0089 are separated by 24 nucleotides EX28DRAFT_0089 and EX28DRAFT_0090 overlap by 113 nucleotides
EX28DRAFT_0087: EX28DRAFT_0087 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 26,122 to 27,483
_0087
EX28DRAFT_0088: EX28DRAFT_0088 - haloacid dehalogenase superfamily, subfamily IF hydrolase, YfhB, at 27,487 to 28,122
_0088
EX28DRAFT_0089: EX28DRAFT_0089 - Cytosine/adenosine deaminases, at 28,147 to 28,686
_0089
EX28DRAFT_0090: EX28DRAFT_0090 - Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein, at 28,574 to 30,124
_0090
Position (kb)
28
29 Strain fitness (log2 ratio)
-1
0
1
2 at 27.315 kb on + strand, within EX28DRAFT_0087 at 27.316 kb on - strand, within EX28DRAFT_0087 at 27.316 kb on - strand, within EX28DRAFT_0087 at 27.319 kb on + strand, within EX28DRAFT_0087 at 27.403 kb on + strand at 27.403 kb on + strand at 27.404 kb on - strand at 27.410 kb on + strand at 27.416 kb on - strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.482 kb on - strand at 27.549 kb on + strand at 27.549 kb on + strand at 27.549 kb on + strand at 27.550 kb on - strand at 27.621 kb on - strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.696 kb on + strand, within EX28DRAFT_0088 at 27.697 kb on - strand, within EX28DRAFT_0088 at 27.805 kb on + strand, within EX28DRAFT_0088 at 28.120 kb on + strand at 28.131 kb on - strand at 28.131 kb on - strand at 28.143 kb on + strand at 28.144 kb on - strand at 28.144 kb on - strand at 28.146 kb on - strand at 28.785 kb on + strand, within EX28DRAFT_0090 at 28.806 kb on + strand, within EX28DRAFT_0090 at 28.807 kb on - strand, within EX28DRAFT_0090 at 28.813 kb on - strand, within EX28DRAFT_0090 at 28.839 kb on + strand, within EX28DRAFT_0090 at 28.853 kb on - strand, within EX28DRAFT_0090 at 28.853 kb on - strand, within EX28DRAFT_0090 at 28.854 kb on + strand, within EX28DRAFT_0090 at 28.855 kb on - strand, within EX28DRAFT_0090 at 28.855 kb on - strand, within EX28DRAFT_0090 at 28.915 kb on + strand, within EX28DRAFT_0090 at 28.955 kb on - strand, within EX28DRAFT_0090 at 28.974 kb on + strand, within EX28DRAFT_0090 at 29.038 kb on + strand, within EX28DRAFT_0090 at 29.039 kb on - strand, within EX28DRAFT_0090 at 29.050 kb on - strand, within EX28DRAFT_0090 at 29.331 kb on + strand, within EX28DRAFT_0090 at 29.356 kb on + strand, within EX28DRAFT_0090 at 29.529 kb on + strand, within EX28DRAFT_0090 at 29.530 kb on - strand, within EX28DRAFT_0090 at 29.683 kb on + strand, within EX28DRAFT_0090
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 27,315 + EX28DRAFT_0087 0.88 +0.3 27,316 - EX28DRAFT_0087 0.88 +0.2 27,316 - EX28DRAFT_0087 0.88 +1.1 27,319 + EX28DRAFT_0087 0.88 -0.9 27,403 + -0.6 27,403 + -0.4 27,404 - -0.8 27,410 + +0.2 27,416 - -0.3 27,420 - -0.1 27,420 - -0.3 27,420 - +1.4 27,420 - +0.1 27,482 - -0.5 27,549 + -0.0 27,549 + -0.8 27,549 + -0.1 27,550 - -0.2 27,621 - EX28DRAFT_0088 0.21 -1.1 27,696 + EX28DRAFT_0088 0.33 +0.2 27,696 + EX28DRAFT_0088 0.33 +0.0 27,696 + EX28DRAFT_0088 0.33 -0.0 27,696 + EX28DRAFT_0088 0.33 +0.2 27,697 - EX28DRAFT_0088 0.33 +0.6 27,805 + EX28DRAFT_0088 0.50 +0.2 28,120 + +0.2 28,131 - +0.7 28,131 - +0.1 28,143 + -1.1 28,144 - -1.2 28,144 - -0.6 28,146 - -0.8 28,785 + EX28DRAFT_0090 0.14 +0.4 28,806 + EX28DRAFT_0090 0.15 +0.9 28,807 - EX28DRAFT_0090 0.15 -0.2 28,813 - EX28DRAFT_0090 0.15 +0.1 28,839 + EX28DRAFT_0090 0.17 -0.1 28,853 - EX28DRAFT_0090 0.18 +0.4 28,853 - EX28DRAFT_0090 0.18 +2.4 28,854 + EX28DRAFT_0090 0.18 -0.2 28,855 - EX28DRAFT_0090 0.18 -0.4 28,855 - EX28DRAFT_0090 0.18 -0.1 28,915 + EX28DRAFT_0090 0.22 +0.0 28,955 - EX28DRAFT_0090 0.25 -0.9 28,974 + EX28DRAFT_0090 0.26 +0.1 29,038 + EX28DRAFT_0090 0.30 -0.4 29,039 - EX28DRAFT_0090 0.30 -0.2 29,050 - EX28DRAFT_0090 0.31 +0.0 29,331 + EX28DRAFT_0090 0.49 -0.1 29,356 + EX28DRAFT_0090 0.50 +1.2 29,529 + EX28DRAFT_0090 0.62 -0.6 29,530 - EX28DRAFT_0090 0.62 +1.5 29,683 + EX28DRAFT_0090 0.72 -0.9
Or see this region's nucleotide sequence