Experiment: 4-Hydroxyvalerate (C) (40mM)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4714 and PP_t49 are separated by 78 nucleotides PP_t49 and PP_t48 are separated by 100 nucleotides PP_t48 and PP_5706 are separated by 21 nucleotides PP_5706 and PP_4715 are separated by 4 nucleotides PP_4715 and PP_4716 are separated by 65 nucleotides
PP_4714: PP_4714 - ribosome maturation factor, at 5,360,329 to 5,360,838
_4714
PP_t49: PP_t49 - tRNA-Met, at 5,360,917 to 5,360,993
_t49
PP_t48: PP_t48 - tRNA-Leu, at 5,361,094 to 5,361,179
_t48
PP_5706: PP_5706 - Sec translocation complex - SecG subunit, at 5,361,201 to 5,361,599
_5706
PP_4715: PP_4715 - triose phosphate isomerase, at 5,361,604 to 5,362,359
_4715
PP_4716: PP_4716 - Phosphoglucosamine mutase, at 5,362,425 to 5,363,765
_4716
Position (kb) 5361
5362
5363 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 5360.957 kb on - strand, within PP_t49 at 5360.973 kb on - strand, within PP_t49 at 5361.186 kb on - strand at 5361.186 kb on - strand at 5361.189 kb on - strand at 5361.189 kb on - strand at 5361.229 kb on - strand at 5361.229 kb on - strand at 5361.229 kb on - strand at 5361.229 kb on - strand at 5361.240 kb on - strand at 5361.291 kb on - strand, within PP_5706 at 5361.291 kb on - strand, within PP_5706 at 5361.354 kb on - strand at 5361.354 kb on - strand, within PP_5706 at 5361.424 kb on - strand, within PP_5706 at 5361.426 kb on - strand, within PP_5706 at 5361.442 kb on - strand, within PP_5706 at 5361.522 kb on - strand, within PP_5706 at 5361.547 kb on - strand, within PP_5706
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxyvalerate (C) (40mM) remove 5,360,957 - PP_t49 0.52 +1.3 5,360,973 - PP_t49 0.73 -0.1 5,361,186 - +0.3 5,361,186 - -0.2 5,361,189 - +0.7 5,361,189 - -0.5 5,361,229 - -1.6 5,361,229 - +0.4 5,361,229 - -0.6 5,361,229 - -1.2 5,361,240 - +1.2 5,361,291 - PP_5706 0.23 +1.8 5,361,291 - PP_5706 0.23 +0.1 5,361,354 - +1.2 5,361,354 - PP_5706 0.38 +2.4 5,361,424 - PP_5706 0.56 +0.7 5,361,426 - PP_5706 0.56 +0.8 5,361,442 - PP_5706 0.60 +3.9 5,361,522 - PP_5706 0.80 +1.0 5,361,547 - PP_5706 0.87 +1.8
Or see this region's nucleotide sequence