Strain Fitness in Pseudomonas putida KT2440 around PP_0323

Experiment: 4-Hydroxyvalerate (C) (40mM)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0322 and PP_0323 are separated by 15 nucleotidesPP_0323 and PP_0324 are separated by 14 nucleotidesPP_0324 and PP_0325 overlap by 4 nucleotides PP_0322: PP_0322 - serine hydroxymethyltransferase, at 386,861 to 388,114 _0322 PP_0323: PP_0323 - sarcosine oxidase subunit beta, at 388,130 to 389,380 _0323 PP_0324: PP_0324 - sarcosine oxidase subunit delta, at 389,395 to 389,730 _0324 PP_0325: PP_0325 - sarcosine oxidase subunit alpha, at 389,727 to 392,741 _0325 Position (kb) 388 389 390Strain fitness (log2 ratio) -2 -1 0 1 2at 387.176 kb on + strand, within PP_0322at 387.278 kb on + strand, within PP_0322at 387.279 kb on - strand, within PP_0322at 387.392 kb on + strand, within PP_0322at 387.393 kb on - strand, within PP_0322at 387.541 kb on + strand, within PP_0322at 387.542 kb on - strand, within PP_0322at 387.883 kb on + strand, within PP_0322at 387.950 kb on - strand, within PP_0322at 388.107 kb on - strandat 388.274 kb on + strand, within PP_0323at 388.275 kb on - strand, within PP_0323at 388.277 kb on + strand, within PP_0323at 388.277 kb on + strand, within PP_0323at 388.328 kb on + strand, within PP_0323at 388.329 kb on - strandat 388.329 kb on - strand, within PP_0323at 388.355 kb on - strand, within PP_0323at 388.379 kb on + strand, within PP_0323at 388.406 kb on + strand, within PP_0323at 388.406 kb on + strand, within PP_0323at 388.407 kb on - strand, within PP_0323at 388.454 kb on + strand, within PP_0323at 388.454 kb on + strand, within PP_0323at 388.455 kb on - strand, within PP_0323at 388.455 kb on - strand, within PP_0323at 388.455 kb on - strand, within PP_0323at 388.455 kb on - strand, within PP_0323at 388.484 kb on + strand, within PP_0323at 388.484 kb on + strand, within PP_0323at 388.484 kb on + strand, within PP_0323at 388.485 kb on - strand, within PP_0323at 388.485 kb on - strand, within PP_0323at 388.605 kb on - strand, within PP_0323at 388.654 kb on - strand, within PP_0323at 388.976 kb on + strand, within PP_0323at 388.976 kb on + strand, within PP_0323at 389.148 kb on + strand, within PP_0323at 389.148 kb on + strand, within PP_0323at 389.149 kb on - strand, within PP_0323at 389.149 kb on - strand, within PP_0323at 389.330 kb on + strandat 389.330 kb on + strandat 389.330 kb on + strandat 389.330 kb on + strandat 389.403 kb on + strandat 389.404 kb on - strandat 389.404 kb on - strandat 389.404 kb on - strandat 389.404 kb on - strandat 389.517 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.521 kb on + strand, within PP_0324at 389.522 kb on - strand, within PP_0324at 389.522 kb on - strand, within PP_0324at 389.522 kb on - strand, within PP_0324at 389.522 kb on - strand, within PP_0324at 389.522 kb on - strand, within PP_0324at 389.522 kb on - strand, within PP_0324at 389.522 kb on - strand, within PP_0324at 389.522 kb on - strand, within PP_0324at 389.522 kb on - strand, within PP_0324at 389.530 kb on + strand, within PP_0324at 389.530 kb on + strand, within PP_0324at 389.530 kb on + strand, within PP_0324at 389.530 kb on + strand, within PP_0324at 389.530 kb on + strand, within PP_0324at 389.593 kb on + strand, within PP_0324at 389.594 kb on - strand, within PP_0324at 389.641 kb on + strand, within PP_0324at 389.642 kb on - strand, within PP_0324at 389.727 kb on - strandat 389.740 kb on - strandat 389.740 kb on - strandat 389.763 kb on - strandat 389.808 kb on + strandat 389.808 kb on + strandat 389.809 kb on - strandat 389.876 kb on + strandat 389.876 kb on + strandat 389.876 kb on + strandat 389.877 kb on - strandat 389.886 kb on + strandat 389.887 kb on - strandat 389.887 kb on - strandat 389.964 kb on - strandat 390.003 kb on + strandat 390.004 kb on - strandat 390.108 kb on + strand, within PP_0325at 390.108 kb on + strand, within PP_0325at 390.111 kb on + strand, within PP_0325at 390.126 kb on + strand, within PP_0325at 390.127 kb on - strand, within PP_0325at 390.151 kb on - strand, within PP_0325at 390.151 kb on - strand, within PP_0325at 390.159 kb on + strand, within PP_0325at 390.160 kb on - strand, within PP_0325at 390.160 kb on - strand, within PP_0325at 390.167 kb on + strand, within PP_0325at 390.167 kb on + strand, within PP_0325at 390.168 kb on - strand, within PP_0325at 390.216 kb on + strand, within PP_0325at 390.216 kb on + strand, within PP_0325at 390.216 kb on + strand, within PP_0325at 390.217 kb on - strand, within PP_0325at 390.217 kb on - strand, within PP_0325at 390.222 kb on + strand, within PP_0325at 390.222 kb on + strand, within PP_0325at 390.222 kb on + strand, within PP_0325at 390.222 kb on + strand, within PP_0325at 390.222 kb on + strand, within PP_0325at 390.223 kb on - strand, within PP_0325

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Per-strain Table

Position Strand Gene LocusTag Fraction 4-Hydroxyvalerate (C) (40mM)
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387,176 + PP_0322 0.25 +0.5
387,278 + PP_0322 0.33 -0.1
387,279 - PP_0322 0.33 -1.0
387,392 + PP_0322 0.42 -0.3
387,393 - PP_0322 0.42 -0.1
387,541 + PP_0322 0.54 +0.3
387,542 - PP_0322 0.54 +1.2
387,883 + PP_0322 0.81 +0.4
387,950 - PP_0322 0.87 -0.8
388,107 - +0.6
388,274 + PP_0323 0.12 +0.5
388,275 - PP_0323 0.12 +0.4
388,277 + PP_0323 0.12 +0.1
388,277 + PP_0323 0.12 -0.4
388,328 + PP_0323 0.16 -1.0
388,329 - -0.3
388,329 - PP_0323 0.16 +0.4
388,355 - PP_0323 0.18 +0.1
388,379 + PP_0323 0.20 +0.5
388,406 + PP_0323 0.22 -1.2
388,406 + PP_0323 0.22 +0.3
388,407 - PP_0323 0.22 +0.5
388,454 + PP_0323 0.26 +0.7
388,454 + PP_0323 0.26 +0.7
388,455 - PP_0323 0.26 -0.7
388,455 - PP_0323 0.26 +0.6
388,455 - PP_0323 0.26 +0.7
388,455 - PP_0323 0.26 +0.4
388,484 + PP_0323 0.28 -0.3
388,484 + PP_0323 0.28 +1.6
388,484 + PP_0323 0.28 +0.4
388,485 - PP_0323 0.28 -0.1
388,485 - PP_0323 0.28 +2.7
388,605 - PP_0323 0.38 -0.0
388,654 - PP_0323 0.42 -0.2
388,976 + PP_0323 0.68 +0.4
388,976 + PP_0323 0.68 -0.6
389,148 + PP_0323 0.81 +1.2
389,148 + PP_0323 0.81 +0.5
389,149 - PP_0323 0.81 +0.5
389,149 - PP_0323 0.81 -0.0
389,330 + -1.0
389,330 + -0.4
389,330 + +0.1
389,330 + +0.8
389,403 + +1.0
389,404 - +0.2
389,404 - +1.1
389,404 - -0.8
389,404 - +0.7
389,517 + PP_0324 0.36 -0.3
389,521 + PP_0324 0.38 +0.7
389,521 + PP_0324 0.38 -1.2
389,521 + PP_0324 0.38 -0.5
389,521 + PP_0324 0.38 -1.2
389,521 + PP_0324 0.38 +0.3
389,521 + PP_0324 0.38 -1.3
389,521 + PP_0324 0.38 -0.1
389,521 + PP_0324 0.38 +0.3
389,521 + PP_0324 0.38 -0.5
389,521 + PP_0324 0.38 +0.3
389,521 + PP_0324 0.38 -0.1
389,522 - PP_0324 0.38 +0.1
389,522 - PP_0324 0.38 +0.7
389,522 - PP_0324 0.38 -0.0
389,522 - PP_0324 0.38 +0.8
389,522 - PP_0324 0.38 +0.2
389,522 - PP_0324 0.38 -0.3
389,522 - PP_0324 0.38 +0.3
389,522 - PP_0324 0.38 -2.4
389,522 - PP_0324 0.38 +0.2
389,530 + PP_0324 0.40 -0.7
389,530 + PP_0324 0.40 +0.7
389,530 + PP_0324 0.40 +1.1
389,530 + PP_0324 0.40 +0.8
389,530 + PP_0324 0.40 -0.3
389,593 + PP_0324 0.59 -0.7
389,594 - PP_0324 0.59 +0.1
389,641 + PP_0324 0.73 -0.4
389,642 - PP_0324 0.74 +0.5
389,727 - +0.1
389,740 - -0.6
389,740 - -0.1
389,763 - +0.8
389,808 + +0.8
389,808 + +0.5
389,809 - -0.3
389,876 + -0.4
389,876 + +0.2
389,876 + +0.0
389,877 - +0.8
389,886 + +0.4
389,887 - -0.1
389,887 - +0.4
389,964 - +0.3
390,003 + -0.3
390,004 - +0.4
390,108 + PP_0325 0.13 +0.4
390,108 + PP_0325 0.13 -1.2
390,111 + PP_0325 0.13 +0.6
390,126 + PP_0325 0.13 +0.3
390,127 - PP_0325 0.13 +0.4
390,151 - PP_0325 0.14 +1.2
390,151 - PP_0325 0.14 +0.4
390,159 + PP_0325 0.14 +0.4
390,160 - PP_0325 0.14 +0.8
390,160 - PP_0325 0.14 +0.6
390,167 + PP_0325 0.15 +0.1
390,167 + PP_0325 0.15 -0.4
390,168 - PP_0325 0.15 -0.8
390,216 + PP_0325 0.16 -0.0
390,216 + PP_0325 0.16 -0.4
390,216 + PP_0325 0.16 -0.3
390,217 - PP_0325 0.16 -1.7
390,217 - PP_0325 0.16 -0.7
390,222 + PP_0325 0.16 -0.6
390,222 + PP_0325 0.16 +0.3
390,222 + PP_0325 0.16 +0.4
390,222 + PP_0325 0.16 +0.1
390,222 + PP_0325 0.16 +0.8
390,223 - PP_0325 0.16 -0.2

Or see this region's nucleotide sequence