Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF922 and GFF923 overlap by 40 nucleotides GFF923 and GFF924 are separated by 137 nucleotides GFF924 and GFF925 overlap by 6 nucleotides GFF925 and GFF926 are separated by 224 nucleotides
GFF922 - PTS system, trehalose-specific IIB component (EC 2.7.1.69) / PTS system, trehalose-specific IIC component (EC 2.7.1.69), at 100,154 to 101,029
GFF922
GFF923 - PTS system, trehalose-specific IIB component (EC 2.7.1.69) / PTS system, trehalose-specific IIC component (EC 2.7.1.69), at 100,990 to 101,571
GFF923
GFF924 - Trehalose operon transcriptional repressor, at 101,709 to 102,656
GFF924
GFF925 - hypothetical protein, at 102,651 to 102,767
GFF925
GFF926 - Mg(2+) transport ATPase, P-type (EC 3.6.3.2), at 102,992 to 105,748
GFF926
Position (kb) 101
102
103 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 100.773 kb on - strand, within GFF922 at 100.893 kb on + strand, within GFF922 at 100.916 kb on + strand, within GFF922 at 101.067 kb on - strand, within GFF923 at 101.096 kb on - strand, within GFF923 at 101.188 kb on + strand, within GFF923 at 101.255 kb on - strand, within GFF923 at 101.315 kb on + strand, within GFF923 at 101.315 kb on + strand, within GFF923 at 101.349 kb on - strand, within GFF923 at 101.423 kb on - strand, within GFF923 at 101.596 kb on + strand at 101.597 kb on - strand at 101.597 kb on - strand at 101.689 kb on + strand at 101.689 kb on + strand at 101.698 kb on + strand at 101.702 kb on + strand at 101.702 kb on + strand at 101.892 kb on - strand, within GFF924 at 101.908 kb on + strand, within GFF924 at 101.908 kb on + strand, within GFF924 at 101.992 kb on + strand, within GFF924 at 102.064 kb on + strand, within GFF924 at 102.065 kb on - strand, within GFF924 at 102.123 kb on - strand, within GFF924 at 102.165 kb on - strand, within GFF924 at 102.337 kb on - strand, within GFF924 at 102.368 kb on + strand, within GFF924 at 102.369 kb on - strand, within GFF924 at 102.372 kb on - strand, within GFF924 at 102.380 kb on - strand, within GFF924 at 102.380 kb on - strand, within GFF924 at 102.458 kb on + strand, within GFF924 at 102.750 kb on - strand, within GFF925 at 102.764 kb on - strand at 103.035 kb on + strand at 103.040 kb on - strand at 103.040 kb on - strand at 103.243 kb on - strand at 103.336 kb on - strand, within GFF926 at 103.603 kb on + strand, within GFF926 at 103.606 kb on + strand, within GFF926
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 100,773 - GFF922 0.71 -1.2 100,893 + GFF922 0.84 -0.6 100,916 + GFF922 0.87 -1.4 101,067 - GFF923 0.13 -1.4 101,096 - GFF923 0.18 +0.3 101,188 + GFF923 0.34 -0.6 101,255 - GFF923 0.46 -0.1 101,315 + GFF923 0.56 -0.2 101,315 + GFF923 0.56 +0.7 101,349 - GFF923 0.62 +0.5 101,423 - GFF923 0.74 -0.7 101,596 + +0.1 101,597 - +0.4 101,597 - +4.9 101,689 + -2.1 101,689 + +0.4 101,698 + -0.6 101,702 + +1.0 101,702 + +0.2 101,892 - GFF924 0.19 +1.1 101,908 + GFF924 0.21 +0.7 101,908 + GFF924 0.21 +1.2 101,992 + GFF924 0.30 +0.6 102,064 + GFF924 0.37 +0.5 102,065 - GFF924 0.38 +3.0 102,123 - GFF924 0.44 +0.4 102,165 - GFF924 0.48 +0.6 102,337 - GFF924 0.66 +0.8 102,368 + GFF924 0.70 +2.2 102,369 - GFF924 0.70 +1.1 102,372 - GFF924 0.70 +1.1 102,380 - GFF924 0.71 +0.1 102,380 - GFF924 0.71 +1.4 102,458 + GFF924 0.79 +1.4 102,750 - GFF925 0.85 -0.9 102,764 - -1.1 103,035 + +0.8 103,040 - -1.4 103,040 - -0.2 103,243 - -1.1 103,336 - GFF926 0.12 -0.2 103,603 + GFF926 0.22 +0.4 103,606 + GFF926 0.22 +0.2
Or see this region's nucleotide sequence