Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF921 and GFF922 are separated by 49 nucleotides GFF922 and GFF923 overlap by 40 nucleotides GFF923 and GFF924 are separated by 137 nucleotides
GFF921 - Trehalose-6-phosphate hydrolase (EC 3.2.1.93), at 98,452 to 100,104
GFF921
GFF922 - PTS system, trehalose-specific IIB component (EC 2.7.1.69) / PTS system, trehalose-specific IIC component (EC 2.7.1.69), at 100,154 to 101,029
GFF922
GFF923 - PTS system, trehalose-specific IIB component (EC 2.7.1.69) / PTS system, trehalose-specific IIC component (EC 2.7.1.69), at 100,990 to 101,571
GFF923
GFF924 - Trehalose operon transcriptional repressor, at 101,709 to 102,656
GFF924
Position (kb) 100
101
102 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 99.218 kb on + strand, within GFF921 at 99.219 kb on - strand, within GFF921 at 99.219 kb on - strand, within GFF921 at 99.321 kb on - strand, within GFF921 at 99.417 kb on + strand, within GFF921 at 99.438 kb on + strand, within GFF921 at 99.441 kb on - strand, within GFF921 at 99.626 kb on - strand, within GFF921 at 99.630 kb on - strand, within GFF921 at 99.633 kb on - strand, within GFF921 at 99.786 kb on + strand, within GFF921 at 99.792 kb on + strand, within GFF921 at 99.793 kb on - strand, within GFF921 at 99.793 kb on - strand, within GFF921 at 99.793 kb on - strand, within GFF921 at 99.807 kb on + strand, within GFF921 at 99.879 kb on - strand, within GFF921 at 99.897 kb on + strand, within GFF921 at 99.899 kb on + strand, within GFF921 at 99.899 kb on + strand, within GFF921 at 99.940 kb on - strand at 100.071 kb on + strand at 100.099 kb on - strand at 100.152 kb on + strand at 100.153 kb on - strand at 100.153 kb on - strand at 100.155 kb on + strand at 100.194 kb on + strand at 100.198 kb on + strand at 100.198 kb on + strand at 100.198 kb on + strand at 100.198 kb on + strand at 100.251 kb on + strand, within GFF922 at 100.410 kb on + strand, within GFF922 at 100.410 kb on + strand, within GFF922 at 100.411 kb on - strand, within GFF922 at 100.437 kb on + strand, within GFF922 at 100.438 kb on - strand, within GFF922 at 100.483 kb on + strand, within GFF922 at 100.773 kb on - strand, within GFF922 at 100.893 kb on + strand, within GFF922 at 100.916 kb on + strand, within GFF922 at 101.067 kb on - strand, within GFF923 at 101.096 kb on - strand, within GFF923 at 101.188 kb on + strand, within GFF923 at 101.255 kb on - strand, within GFF923 at 101.315 kb on + strand, within GFF923 at 101.315 kb on + strand, within GFF923 at 101.349 kb on - strand, within GFF923 at 101.423 kb on - strand, within GFF923 at 101.596 kb on + strand at 101.597 kb on - strand at 101.597 kb on - strand at 101.689 kb on + strand at 101.689 kb on + strand at 101.698 kb on + strand at 101.702 kb on + strand at 101.702 kb on + strand at 101.892 kb on - strand, within GFF924 at 101.908 kb on + strand, within GFF924 at 101.908 kb on + strand, within GFF924 at 101.992 kb on + strand, within GFF924
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 99,218 + GFF921 0.46 -0.2 99,219 - GFF921 0.46 +3.0 99,219 - GFF921 0.46 -0.1 99,321 - GFF921 0.53 +0.3 99,417 + GFF921 0.58 +0.0 99,438 + GFF921 0.60 -0.8 99,441 - GFF921 0.60 -1.0 99,626 - GFF921 0.71 -0.5 99,630 - GFF921 0.71 +0.2 99,633 - GFF921 0.71 +2.0 99,786 + GFF921 0.81 +0.4 99,792 + GFF921 0.81 -0.4 99,793 - GFF921 0.81 +0.3 99,793 - GFF921 0.81 +0.0 99,793 - GFF921 0.81 -0.7 99,807 + GFF921 0.82 -1.0 99,879 - GFF921 0.86 +0.3 99,897 + GFF921 0.87 -0.6 99,899 + GFF921 0.88 -1.9 99,899 + GFF921 0.88 +1.4 99,940 - -1.2 100,071 + -0.2 100,099 - -0.8 100,152 + +0.2 100,153 - +1.3 100,153 - -0.1 100,155 + -1.0 100,194 + -2.3 100,198 + +0.6 100,198 + -2.5 100,198 + +2.5 100,198 + +2.4 100,251 + GFF922 0.11 +0.2 100,410 + GFF922 0.29 +2.0 100,410 + GFF922 0.29 -1.7 100,411 - GFF922 0.29 +0.9 100,437 + GFF922 0.32 +0.8 100,438 - GFF922 0.32 +0.0 100,483 + GFF922 0.38 -0.9 100,773 - GFF922 0.71 -1.2 100,893 + GFF922 0.84 -0.6 100,916 + GFF922 0.87 -1.4 101,067 - GFF923 0.13 -1.4 101,096 - GFF923 0.18 +0.3 101,188 + GFF923 0.34 -0.6 101,255 - GFF923 0.46 -0.1 101,315 + GFF923 0.56 -0.2 101,315 + GFF923 0.56 +0.7 101,349 - GFF923 0.62 +0.5 101,423 - GFF923 0.74 -0.7 101,596 + +0.1 101,597 - +0.4 101,597 - +4.9 101,689 + -2.1 101,689 + +0.4 101,698 + -0.6 101,702 + +1.0 101,702 + +0.2 101,892 - GFF924 0.19 +1.1 101,908 + GFF924 0.21 +0.7 101,908 + GFF924 0.21 +1.2 101,992 + GFF924 0.30 +0.6
Or see this region's nucleotide sequence