Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF919 and GFF920 are separated by 204 nucleotides GFF920 and GFF921 are separated by 33 nucleotides GFF921 and GFF922 are separated by 49 nucleotides GFF922 and GFF923 overlap by 40 nucleotides
GFF919 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 95,905 to 98,043
GFF919
GFF920 - FIG01045722: hypothetical protein, at 98,248 to 98,418
GFF920
GFF921 - Trehalose-6-phosphate hydrolase (EC 3.2.1.93), at 98,452 to 100,104
GFF921
GFF922 - PTS system, trehalose-specific IIB component (EC 2.7.1.69) / PTS system, trehalose-specific IIC component (EC 2.7.1.69), at 100,154 to 101,029
GFF922
GFF923 - PTS system, trehalose-specific IIB component (EC 2.7.1.69) / PTS system, trehalose-specific IIC component (EC 2.7.1.69), at 100,990 to 101,571
GFF923
Position (kb) 98
99
100
101 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 97.479 kb on + strand, within GFF919 at 97.518 kb on + strand, within GFF919 at 97.518 kb on + strand, within GFF919 at 97.519 kb on - strand, within GFF919 at 97.519 kb on - strand, within GFF919 at 97.547 kb on + strand, within GFF919 at 97.562 kb on + strand, within GFF919 at 97.591 kb on + strand, within GFF919 at 97.594 kb on + strand, within GFF919 at 97.594 kb on + strand, within GFF919 at 97.625 kb on + strand, within GFF919 at 97.678 kb on - strand, within GFF919 at 97.793 kb on + strand, within GFF919 at 97.932 kb on - strand at 97.969 kb on + strand at 97.983 kb on + strand at 98.242 kb on + strand at 98.439 kb on + strand at 98.439 kb on + strand at 98.439 kb on + strand at 98.439 kb on + strand at 98.439 kb on + strand at 98.440 kb on - strand at 98.453 kb on + strand at 98.461 kb on + strand at 98.462 kb on - strand at 98.526 kb on - strand at 98.530 kb on - strand at 98.560 kb on - strand at 98.560 kb on - strand at 98.648 kb on - strand, within GFF921 at 98.733 kb on - strand, within GFF921 at 98.738 kb on + strand, within GFF921 at 98.739 kb on - strand, within GFF921 at 98.790 kb on - strand, within GFF921 at 99.030 kb on + strand, within GFF921 at 99.030 kb on + strand, within GFF921 at 99.031 kb on - strand, within GFF921 at 99.031 kb on - strand, within GFF921 at 99.031 kb on - strand, within GFF921 at 99.031 kb on - strand, within GFF921 at 99.033 kb on - strand, within GFF921 at 99.093 kb on - strand, within GFF921 at 99.218 kb on + strand, within GFF921 at 99.219 kb on - strand, within GFF921 at 99.219 kb on - strand, within GFF921 at 99.321 kb on - strand, within GFF921 at 99.417 kb on + strand, within GFF921 at 99.438 kb on + strand, within GFF921 at 99.441 kb on - strand, within GFF921 at 99.626 kb on - strand, within GFF921 at 99.630 kb on - strand, within GFF921 at 99.633 kb on - strand, within GFF921 at 99.786 kb on + strand, within GFF921 at 99.792 kb on + strand, within GFF921 at 99.793 kb on - strand, within GFF921 at 99.793 kb on - strand, within GFF921 at 99.793 kb on - strand, within GFF921 at 99.807 kb on + strand, within GFF921 at 99.879 kb on - strand, within GFF921 at 99.897 kb on + strand, within GFF921 at 99.899 kb on + strand, within GFF921 at 99.899 kb on + strand, within GFF921 at 99.940 kb on - strand at 100.071 kb on + strand at 100.099 kb on - strand at 100.152 kb on + strand at 100.153 kb on - strand at 100.153 kb on - strand at 100.155 kb on + strand at 100.194 kb on + strand at 100.198 kb on + strand at 100.198 kb on + strand at 100.198 kb on + strand at 100.198 kb on + strand at 100.251 kb on + strand, within GFF922 at 100.410 kb on + strand, within GFF922 at 100.410 kb on + strand, within GFF922 at 100.411 kb on - strand, within GFF922 at 100.437 kb on + strand, within GFF922 at 100.438 kb on - strand, within GFF922 at 100.483 kb on + strand, within GFF922 at 100.773 kb on - strand, within GFF922 at 100.893 kb on + strand, within GFF922 at 100.916 kb on + strand, within GFF922 at 101.067 kb on - strand, within GFF923 at 101.096 kb on - strand, within GFF923
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 97,479 + GFF919 0.74 +0.1 97,518 + GFF919 0.75 +1.1 97,518 + GFF919 0.75 -1.6 97,519 - GFF919 0.75 +0.9 97,519 - GFF919 0.75 -0.1 97,547 + GFF919 0.77 -1.3 97,562 + GFF919 0.77 +0.5 97,591 + GFF919 0.79 +0.1 97,594 + GFF919 0.79 -1.5 97,594 + GFF919 0.79 +0.0 97,625 + GFF919 0.80 +0.0 97,678 - GFF919 0.83 -0.4 97,793 + GFF919 0.88 +1.2 97,932 - -4.3 97,969 + -0.4 97,983 + +0.5 98,242 + +1.9 98,439 + -0.4 98,439 + +1.0 98,439 + +0.3 98,439 + -0.5 98,439 + +0.4 98,440 - -1.4 98,453 + -2.1 98,461 + -0.5 98,462 - +1.0 98,526 - +0.7 98,530 - +0.1 98,560 - +0.6 98,560 - -0.8 98,648 - GFF921 0.12 -3.2 98,733 - GFF921 0.17 +0.1 98,738 + GFF921 0.17 +0.5 98,739 - GFF921 0.17 -0.1 98,790 - GFF921 0.20 +0.1 99,030 + GFF921 0.35 +0.6 99,030 + GFF921 0.35 +0.2 99,031 - GFF921 0.35 +0.3 99,031 - GFF921 0.35 +1.2 99,031 - GFF921 0.35 -2.2 99,031 - GFF921 0.35 -0.3 99,033 - GFF921 0.35 -1.7 99,093 - GFF921 0.39 -1.1 99,218 + GFF921 0.46 -0.2 99,219 - GFF921 0.46 +3.0 99,219 - GFF921 0.46 -0.1 99,321 - GFF921 0.53 +0.3 99,417 + GFF921 0.58 +0.0 99,438 + GFF921 0.60 -0.8 99,441 - GFF921 0.60 -1.0 99,626 - GFF921 0.71 -0.5 99,630 - GFF921 0.71 +0.2 99,633 - GFF921 0.71 +2.0 99,786 + GFF921 0.81 +0.4 99,792 + GFF921 0.81 -0.4 99,793 - GFF921 0.81 +0.3 99,793 - GFF921 0.81 +0.0 99,793 - GFF921 0.81 -0.7 99,807 + GFF921 0.82 -1.0 99,879 - GFF921 0.86 +0.3 99,897 + GFF921 0.87 -0.6 99,899 + GFF921 0.88 -1.9 99,899 + GFF921 0.88 +1.4 99,940 - -1.2 100,071 + -0.2 100,099 - -0.8 100,152 + +0.2 100,153 - +1.3 100,153 - -0.1 100,155 + -1.0 100,194 + -2.3 100,198 + +0.6 100,198 + -2.5 100,198 + +2.5 100,198 + +2.4 100,251 + GFF922 0.11 +0.2 100,410 + GFF922 0.29 +2.0 100,410 + GFF922 0.29 -1.7 100,411 - GFF922 0.29 +0.9 100,437 + GFF922 0.32 +0.8 100,438 - GFF922 0.32 +0.0 100,483 + GFF922 0.38 -0.9 100,773 - GFF922 0.71 -1.2 100,893 + GFF922 0.84 -0.6 100,916 + GFF922 0.87 -1.4 101,067 - GFF923 0.13 -1.4 101,096 - GFF923 0.18 +0.3
Or see this region's nucleotide sequence