Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF709 and GFF710 are separated by 353 nucleotides GFF710 and GFF711 are separated by 62 nucleotides GFF711 and GFF712 are separated by 0 nucleotides GFF712 and GFF713 are separated by 36 nucleotides GFF713 and GFF714 are separated by 242 nucleotides
GFF709 - Putative DMT superfamily metabolite efflux protein precursor, at 65,357 to 66,244
GFF709
GFF710 - Outer membrane protein X precursor, at 66,598 to 67,113
GFF710
GFF711 - FIG00732228: membrane protein, at 67,176 to 68,756
GFF711
GFF712 - hypothetical protein, at 68,757 to 68,885
GFF712
GFF713 - FIG01047037: hypothetical protein, at 68,922 to 69,092
GFF713
GFF714 - Mn-dependent transcriptional regulator MntR, at 69,335 to 69,808
GFF714
Position (kb) 67
68
69 Strain fitness (log2 ratio) -2
-1
0
1
2 at 66.226 kb on + strand at 66.395 kb on - strand at 66.429 kb on - strand at 66.439 kb on + strand at 66.495 kb on + strand at 66.573 kb on + strand at 66.573 kb on + strand at 66.662 kb on + strand, within GFF710 at 66.675 kb on + strand, within GFF710 at 66.675 kb on + strand, within GFF710 at 66.748 kb on + strand, within GFF710 at 66.749 kb on - strand, within GFF710 at 66.836 kb on - strand, within GFF710 at 66.904 kb on + strand, within GFF710 at 66.979 kb on + strand, within GFF710 at 66.992 kb on - strand, within GFF710 at 67.065 kb on + strand at 67.111 kb on + strand at 67.247 kb on - strand at 67.308 kb on - strand at 67.364 kb on + strand, within GFF711 at 67.382 kb on + strand, within GFF711 at 67.501 kb on + strand, within GFF711 at 67.501 kb on + strand, within GFF711 at 67.582 kb on + strand, within GFF711 at 67.624 kb on + strand, within GFF711 at 67.624 kb on + strand, within GFF711 at 67.625 kb on - strand, within GFF711 at 67.637 kb on + strand, within GFF711 at 67.916 kb on - strand, within GFF711 at 67.990 kb on + strand, within GFF711 at 68.101 kb on + strand, within GFF711 at 68.101 kb on + strand, within GFF711 at 68.102 kb on - strand, within GFF711 at 68.149 kb on - strand, within GFF711 at 68.249 kb on + strand, within GFF711 at 68.255 kb on + strand, within GFF711 at 68.423 kb on + strand, within GFF711 at 68.424 kb on - strand, within GFF711 at 68.425 kb on + strand, within GFF711 at 68.425 kb on + strand, within GFF711 at 68.465 kb on + strand, within GFF711 at 68.495 kb on + strand, within GFF711 at 68.633 kb on - strand at 68.808 kb on - strand, within GFF712 at 68.808 kb on - strand, within GFF712 at 69.066 kb on - strand, within GFF713 at 69.077 kb on + strand at 69.078 kb on - strand at 69.129 kb on - strand at 69.129 kb on - strand at 69.149 kb on + strand at 69.149 kb on + strand at 69.150 kb on - strand at 69.151 kb on + strand at 69.151 kb on + strand at 69.201 kb on - strand at 69.201 kb on - strand at 69.201 kb on - strand at 69.205 kb on - strand at 69.211 kb on - strand at 69.212 kb on + strand at 69.278 kb on + strand at 69.278 kb on + strand at 69.390 kb on + strand, within GFF714 at 69.391 kb on - strand, within GFF714 at 69.391 kb on - strand, within GFF714 at 69.448 kb on - strand, within GFF714 at 69.456 kb on - strand, within GFF714 at 69.696 kb on + strand, within GFF714
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 66,226 + -0.5 66,395 - -1.0 66,429 - +0.5 66,439 + -1.1 66,495 + +0.0 66,573 + -1.6 66,573 + -0.4 66,662 + GFF710 0.12 +1.5 66,675 + GFF710 0.15 +0.8 66,675 + GFF710 0.15 +0.5 66,748 + GFF710 0.29 +0.6 66,749 - GFF710 0.29 +0.2 66,836 - GFF710 0.46 -0.6 66,904 + GFF710 0.59 +0.6 66,979 + GFF710 0.74 -0.2 66,992 - GFF710 0.76 +1.7 67,065 + -0.2 67,111 + -0.9 67,247 - -0.4 67,308 - -1.3 67,364 + GFF711 0.12 +0.7 67,382 + GFF711 0.13 -0.2 67,501 + GFF711 0.21 -0.8 67,501 + GFF711 0.21 +0.3 67,582 + GFF711 0.26 -0.6 67,624 + GFF711 0.28 +0.2 67,624 + GFF711 0.28 -0.4 67,625 - GFF711 0.28 +0.4 67,637 + GFF711 0.29 -0.6 67,916 - GFF711 0.47 -0.4 67,990 + GFF711 0.51 +0.4 68,101 + GFF711 0.59 +1.0 68,101 + GFF711 0.59 -1.0 68,102 - GFF711 0.59 -1.2 68,149 - GFF711 0.62 -2.1 68,249 + GFF711 0.68 -1.0 68,255 + GFF711 0.68 +0.0 68,423 + GFF711 0.79 -0.4 68,424 - GFF711 0.79 -0.1 68,425 + GFF711 0.79 +1.1 68,425 + GFF711 0.79 -1.1 68,465 + GFF711 0.82 -0.8 68,495 + GFF711 0.83 +0.4 68,633 - +0.1 68,808 - GFF712 0.40 -0.5 68,808 - GFF712 0.40 +0.3 69,066 - GFF713 0.84 +1.3 69,077 + +1.8 69,078 - +1.0 69,129 - +0.1 69,129 - +1.5 69,149 + -0.0 69,149 + +1.2 69,150 - -0.9 69,151 + +1.7 69,151 + +1.2 69,201 - -1.6 69,201 - +0.0 69,201 - +1.0 69,205 - -0.5 69,211 - -0.8 69,212 + -1.2 69,278 + -1.8 69,278 + -1.9 69,390 + GFF714 0.12 -2.5 69,391 - GFF714 0.12 -1.4 69,391 - GFF714 0.12 +0.1 69,448 - GFF714 0.24 -1.7 69,456 - GFF714 0.26 -0.5 69,696 + GFF714 0.76 -0.9
Or see this region's nucleotide sequence