Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF674 and GFF675 are separated by 650 nucleotides GFF675 and GFF676 are separated by 489 nucleotides
GFF674 - Dethiobiotin synthetase (EC 6.3.3.3), at 30,160 to 30,846
GFF674
GFF675 - Excinuclease ABC subunit B, at 31,497 to 33,518
GFF675
GFF676 - invasion plasmid antigen / internalin, putative, at 34,008 to 36,305
GFF676
Position (kb)
31
32
33
34 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 30.525 kb on + strand, within GFF674 at 30.615 kb on + strand, within GFF674 at 30.615 kb on + strand, within GFF674 at 30.616 kb on - strand, within GFF674 at 30.618 kb on + strand, within GFF674 at 30.692 kb on + strand, within GFF674 at 30.693 kb on - strand, within GFF674 at 30.693 kb on - strand, within GFF674 at 30.815 kb on - strand at 30.826 kb on - strand at 31.109 kb on - strand at 31.358 kb on + strand at 31.392 kb on - strand at 31.392 kb on - strand at 31.502 kb on + strand at 31.556 kb on + strand at 31.770 kb on + strand, within GFF675 at 31.770 kb on + strand, within GFF675 at 31.773 kb on + strand, within GFF675 at 31.797 kb on + strand, within GFF675 at 31.798 kb on - strand, within GFF675 at 31.850 kb on + strand, within GFF675 at 31.932 kb on + strand, within GFF675 at 31.956 kb on + strand, within GFF675 at 32.070 kb on - strand, within GFF675 at 32.219 kb on + strand, within GFF675 at 32.219 kb on + strand, within GFF675 at 32.219 kb on + strand, within GFF675 at 32.220 kb on - strand, within GFF675 at 32.220 kb on - strand, within GFF675 at 32.220 kb on - strand, within GFF675 at 32.223 kb on - strand, within GFF675 at 32.245 kb on + strand, within GFF675 at 32.245 kb on + strand, within GFF675 at 32.246 kb on - strand, within GFF675 at 32.285 kb on - strand, within GFF675 at 32.377 kb on + strand, within GFF675 at 32.536 kb on + strand, within GFF675 at 32.536 kb on + strand, within GFF675 at 32.668 kb on - strand, within GFF675 at 32.691 kb on + strand, within GFF675 at 32.691 kb on + strand, within GFF675 at 32.691 kb on + strand, within GFF675 at 32.691 kb on + strand, within GFF675 at 32.852 kb on + strand, within GFF675 at 32.853 kb on - strand, within GFF675 at 32.853 kb on - strand, within GFF675 at 32.933 kb on + strand, within GFF675 at 33.069 kb on - strand, within GFF675 at 33.128 kb on + strand, within GFF675 at 33.228 kb on + strand, within GFF675 at 33.229 kb on - strand, within GFF675 at 33.549 kb on - strand at 33.581 kb on + strand at 33.591 kb on + strand at 33.592 kb on - strand at 33.773 kb on - strand at 33.776 kb on + strand at 33.796 kb on + strand at 33.796 kb on + strand at 33.804 kb on + strand at 33.805 kb on - strand at 33.840 kb on - strand at 34.013 kb on + strand at 34.222 kb on + strand at 34.231 kb on + strand at 34.257 kb on - strand, within GFF676 at 34.349 kb on + strand, within GFF676
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 30,525 + GFF674 0.53 -1.2 30,615 + GFF674 0.66 -0.2 30,615 + GFF674 0.66 +0.6 30,616 - GFF674 0.66 -1.4 30,618 + GFF674 0.67 +0.2 30,692 + GFF674 0.77 -1.1 30,693 - GFF674 0.78 +0.3 30,693 - GFF674 0.78 +1.3 30,815 - -0.2 30,826 - -3.7 31,109 - -0.7 31,358 + -0.1 31,392 - +1.6 31,392 - -0.7 31,502 + -1.8 31,556 + -0.2 31,770 + GFF675 0.14 -0.1 31,770 + GFF675 0.14 +1.6 31,773 + GFF675 0.14 -0.8 31,797 + GFF675 0.15 -0.3 31,798 - GFF675 0.15 -0.4 31,850 + GFF675 0.17 +1.0 31,932 + GFF675 0.22 -2.1 31,956 + GFF675 0.23 -0.7 32,070 - GFF675 0.28 -0.3 32,219 + GFF675 0.36 -0.8 32,219 + GFF675 0.36 -3.4 32,219 + GFF675 0.36 +1.0 32,220 - GFF675 0.36 -0.1 32,220 - GFF675 0.36 +1.9 32,220 - GFF675 0.36 -0.8 32,223 - GFF675 0.36 +0.4 32,245 + GFF675 0.37 +0.6 32,245 + GFF675 0.37 -2.6 32,246 - GFF675 0.37 -1.1 32,285 - GFF675 0.39 -0.2 32,377 + GFF675 0.44 -0.7 32,536 + GFF675 0.51 -0.6 32,536 + GFF675 0.51 -1.1 32,668 - GFF675 0.58 +0.5 32,691 + GFF675 0.59 -0.5 32,691 + GFF675 0.59 -0.5 32,691 + GFF675 0.59 -0.5 32,691 + GFF675 0.59 -1.0 32,852 + GFF675 0.67 -0.8 32,853 - GFF675 0.67 +1.1 32,853 - GFF675 0.67 -0.2 32,933 + GFF675 0.71 -1.6 33,069 - GFF675 0.78 +0.2 33,128 + GFF675 0.81 -0.5 33,228 + GFF675 0.86 -3.7 33,229 - GFF675 0.86 -1.6 33,549 - +0.8 33,581 + +0.2 33,591 + +0.1 33,592 - +2.7 33,773 - +0.4 33,776 + +1.1 33,796 + -0.4 33,796 + +1.2 33,804 + -0.4 33,805 - -0.2 33,840 - -0.5 34,013 + +0.5 34,222 + -0.1 34,231 + +0.4 34,257 - GFF676 0.11 -0.9 34,349 + GFF676 0.15 +1.2
Or see this region's nucleotide sequence