Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF5

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4 and GFF5 are separated by 24 nucleotidesGFF5 and GFF6 overlap by 4 nucleotides GFF4 - Quinolinate synthetase (EC 2.5.1.72), at 967 to 2,010 GFF4 GFF5 - Ribosyl nicotinamide transporter, PnuC-like, at 2,035 to 2,754 GFF5 GFF6 - Cobalt-zinc-cadmium resistance protein CzcD, at 2,751 to 3,689 GFF6 Position (kb) 2 3Strain fitness (log2 ratio) -2 -1 0 1at 1.373 kb on + strand, within GFF4at 1.381 kb on + strand, within GFF4at 1.381 kb on + strand, within GFF4at 1.386 kb on + strand, within GFF4at 1.566 kb on + strand, within GFF4at 1.566 kb on + strand, within GFF4at 1.704 kb on + strand, within GFF4at 1.704 kb on + strand, within GFF4at 2.049 kb on + strandat 2.142 kb on + strand, within GFF5at 2.281 kb on + strand, within GFF5at 2.378 kb on + strand, within GFF5at 2.387 kb on + strand, within GFF5at 2.387 kb on + strand, within GFF5at 2.387 kb on + strand, within GFF5at 2.388 kb on - strand, within GFF5at 2.413 kb on - strand, within GFF5at 2.492 kb on + strand, within GFF5at 2.544 kb on + strand, within GFF5at 2.575 kb on + strand, within GFF5at 2.576 kb on - strand, within GFF5at 2.819 kb on - strandat 2.852 kb on - strand, within GFF6at 2.877 kb on - strand, within GFF6at 2.974 kb on - strand, within GFF6at 2.974 kb on - strand, within GFF6at 2.974 kb on - strand, within GFF6at 3.015 kb on + strand, within GFF6at 3.024 kb on - strand, within GFF6at 3.531 kb on - strand, within GFF6at 3.642 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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1,373 + GFF4 0.39 +0.3
1,381 + GFF4 0.40 +0.4
1,381 + GFF4 0.40 -0.8
1,386 + GFF4 0.40 -1.1
1,566 + GFF4 0.57 +0.7
1,566 + GFF4 0.57 -0.8
1,704 + GFF4 0.71 +0.0
1,704 + GFF4 0.71 -0.7
2,049 + +0.0
2,142 + GFF5 0.15 -2.4
2,281 + GFF5 0.34 -0.1
2,378 + GFF5 0.48 +0.7
2,387 + GFF5 0.49 +0.9
2,387 + GFF5 0.49 +0.0
2,387 + GFF5 0.49 +1.0
2,388 - GFF5 0.49 +0.7
2,413 - GFF5 0.53 +0.5
2,492 + GFF5 0.63 +0.3
2,544 + GFF5 0.71 -0.1
2,575 + GFF5 0.75 -0.0
2,576 - GFF5 0.75 +0.9
2,819 - -0.6
2,852 - GFF6 0.11 -0.3
2,877 - GFF6 0.13 +0.6
2,974 - GFF6 0.24 -1.6
2,974 - GFF6 0.24 +1.0
2,974 - GFF6 0.24 -0.3
3,015 + GFF6 0.28 +0.8
3,024 - GFF6 0.29 +1.1
3,531 - GFF6 0.83 +1.0
3,642 - +0.3

Or see this region's nucleotide sequence