Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4859 and GFF4860 are separated by 100 nucleotides GFF4860 and GFF4861 are separated by 1 nucleotides GFF4861 and GFF4862 are separated by 57 nucleotides
GFF4859 - L-fucose isomerase (EC 5.3.1.25), at 158,185 to 159,960
GFF4859
GFF4860 - L-fuculokinase (EC 2.7.1.51), at 160,061 to 161,479
GFF4860
GFF4861 - L-fucose mutarotase, at 161,481 to 161,903
GFF4861
GFF4862 - L-fucose operon activator, at 161,961 to 162,671
GFF4862
Position (kb) 160
161
162 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 159.145 kb on - strand, within GFF4859 at 159.277 kb on + strand, within GFF4859 at 159.345 kb on + strand, within GFF4859 at 159.345 kb on + strand, within GFF4859 at 159.357 kb on + strand, within GFF4859 at 159.357 kb on + strand, within GFF4859 at 159.390 kb on + strand, within GFF4859 at 159.503 kb on - strand, within GFF4859 at 159.676 kb on - strand, within GFF4859 at 159.684 kb on + strand, within GFF4859 at 159.684 kb on + strand, within GFF4859 at 159.684 kb on + strand, within GFF4859 at 159.688 kb on - strand, within GFF4859 at 159.791 kb on + strand at 159.808 kb on - strand at 159.837 kb on - strand at 159.840 kb on + strand at 159.840 kb on + strand at 159.840 kb on + strand at 159.841 kb on - strand at 159.841 kb on - strand at 159.841 kb on - strand at 159.841 kb on - strand at 159.841 kb on - strand at 159.949 kb on + strand at 160.014 kb on - strand at 160.057 kb on - strand at 160.061 kb on - strand at 160.300 kb on + strand, within GFF4860 at 160.300 kb on + strand, within GFF4860 at 160.301 kb on - strand, within GFF4860 at 160.411 kb on + strand, within GFF4860 at 160.537 kb on + strand, within GFF4860 at 160.592 kb on - strand, within GFF4860 at 160.946 kb on + strand, within GFF4860 at 160.946 kb on + strand, within GFF4860 at 160.948 kb on + strand, within GFF4860 at 160.948 kb on + strand, within GFF4860 at 161.138 kb on - strand, within GFF4860 at 161.247 kb on + strand, within GFF4860 at 161.424 kb on - strand at 161.436 kb on - strand at 161.672 kb on + strand, within GFF4861 at 161.673 kb on - strand, within GFF4861 at 162.044 kb on + strand, within GFF4862 at 162.085 kb on + strand, within GFF4862 at 162.125 kb on + strand, within GFF4862 at 162.126 kb on - strand, within GFF4862 at 162.130 kb on - strand, within GFF4862 at 162.130 kb on - strand, within GFF4862 at 162.232 kb on + strand, within GFF4862 at 162.280 kb on - strand, within GFF4862 at 162.336 kb on - strand, within GFF4862 at 162.336 kb on - strand, within GFF4862 at 162.336 kb on - strand, within GFF4862 at 162.405 kb on + strand, within GFF4862 at 162.414 kb on + strand, within GFF4862
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 159,145 - GFF4859 0.54 +1.2 159,277 + GFF4859 0.61 +0.7 159,345 + GFF4859 0.65 +0.1 159,345 + GFF4859 0.65 +0.1 159,357 + GFF4859 0.66 -0.7 159,357 + GFF4859 0.66 -1.2 159,390 + GFF4859 0.68 +0.9 159,503 - GFF4859 0.74 -0.1 159,676 - GFF4859 0.84 -1.6 159,684 + GFF4859 0.84 +0.3 159,684 + GFF4859 0.84 -0.1 159,684 + GFF4859 0.84 -0.8 159,688 - GFF4859 0.85 -0.4 159,791 + -0.2 159,808 - +2.9 159,837 - +0.3 159,840 + -0.2 159,840 + +0.1 159,840 + -1.4 159,841 - -1.1 159,841 - +0.2 159,841 - +0.4 159,841 - +0.1 159,841 - +0.2 159,949 + -0.6 160,014 - +2.2 160,057 - -2.1 160,061 - -0.2 160,300 + GFF4860 0.17 -0.2 160,300 + GFF4860 0.17 +0.9 160,301 - GFF4860 0.17 +0.2 160,411 + GFF4860 0.25 -0.7 160,537 + GFF4860 0.34 -1.8 160,592 - GFF4860 0.37 +0.5 160,946 + GFF4860 0.62 -0.6 160,946 + GFF4860 0.62 +0.2 160,948 + GFF4860 0.63 +0.7 160,948 + GFF4860 0.63 +0.6 161,138 - GFF4860 0.76 +0.4 161,247 + GFF4860 0.84 -2.3 161,424 - -0.9 161,436 - -0.1 161,672 + GFF4861 0.45 -0.3 161,673 - GFF4861 0.45 -0.7 162,044 + GFF4862 0.12 -1.0 162,085 + GFF4862 0.17 -1.3 162,125 + GFF4862 0.23 -0.9 162,126 - GFF4862 0.23 +0.6 162,130 - GFF4862 0.24 +2.2 162,130 - GFF4862 0.24 +0.2 162,232 + GFF4862 0.38 +0.2 162,280 - GFF4862 0.45 +0.1 162,336 - GFF4862 0.53 -0.8 162,336 - GFF4862 0.53 -0.2 162,336 - GFF4862 0.53 -1.3 162,405 + GFF4862 0.62 +0.7 162,414 + GFF4862 0.64 -0.8
Or see this region's nucleotide sequence