Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4848 and GFF4849 are separated by 68 nucleotides GFF4849 and GFF4850 are separated by 112 nucleotides
GFF4848 - Syd protein, at 147,233 to 147,778
GFF4848
GFF4849 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 147,847 to 148,695
GFF4849
GFF4850 - Decarboxylase family protein, at 148,808 to 150,172
GFF4850
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 146.953 kb on - strand at 147.032 kb on - strand at 147.057 kb on - strand at 147.289 kb on + strand, within GFF4848 at 147.480 kb on - strand, within GFF4848 at 147.484 kb on + strand, within GFF4848 at 147.560 kb on + strand, within GFF4848 at 147.561 kb on - strand, within GFF4848 at 147.561 kb on - strand, within GFF4848 at 147.566 kb on - strand, within GFF4848 at 147.630 kb on + strand, within GFF4848 at 147.672 kb on - strand, within GFF4848 at 147.672 kb on - strand, within GFF4848 at 147.807 kb on - strand at 147.819 kb on + strand at 147.824 kb on + strand at 148.024 kb on + strand, within GFF4849 at 148.101 kb on + strand, within GFF4849 at 148.325 kb on - strand, within GFF4849 at 148.376 kb on + strand, within GFF4849 at 148.522 kb on - strand, within GFF4849 at 148.544 kb on + strand, within GFF4849 at 148.610 kb on - strand, within GFF4849 at 148.731 kb on + strand at 148.731 kb on + strand at 148.732 kb on - strand at 148.761 kb on - strand at 148.765 kb on + strand at 148.796 kb on + strand at 148.813 kb on + strand at 148.819 kb on + strand at 148.819 kb on + strand at 148.844 kb on - strand at 148.844 kb on - strand at 148.934 kb on - strand at 148.996 kb on + strand, within GFF4850 at 149.001 kb on + strand, within GFF4850 at 149.334 kb on + strand, within GFF4850 at 149.561 kb on - strand, within GFF4850 at 149.606 kb on + strand, within GFF4850
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 146,953 - -1.9 147,032 - -1.0 147,057 - -1.9 147,289 + GFF4848 0.10 +3.3 147,480 - GFF4848 0.45 +0.5 147,484 + GFF4848 0.46 +2.1 147,560 + GFF4848 0.60 +2.6 147,561 - GFF4848 0.60 +0.8 147,561 - GFF4848 0.60 +0.6 147,566 - GFF4848 0.61 -1.2 147,630 + GFF4848 0.73 +1.0 147,672 - GFF4848 0.80 +2.4 147,672 - GFF4848 0.80 +0.6 147,807 - -1.9 147,819 + -0.4 147,824 + -0.5 148,024 + GFF4849 0.21 -1.5 148,101 + GFF4849 0.30 +0.0 148,325 - GFF4849 0.56 +0.7 148,376 + GFF4849 0.62 -0.2 148,522 - GFF4849 0.80 +1.1 148,544 + GFF4849 0.82 +1.5 148,610 - GFF4849 0.90 -0.8 148,731 + -0.2 148,731 + +0.0 148,732 - +0.9 148,761 - +0.5 148,765 + +1.1 148,796 + +0.4 148,813 + +0.0 148,819 + -1.1 148,819 + -0.8 148,844 - +0.0 148,844 - -0.7 148,934 - -0.1 148,996 + GFF4850 0.14 +0.9 149,001 + GFF4850 0.14 +0.1 149,334 + GFF4850 0.39 -2.2 149,561 - GFF4850 0.55 +0.6 149,606 + GFF4850 0.58 +0.1
Or see this region's nucleotide sequence