Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4733 and GFF4734 are separated by 344 nucleotides GFF4734 and GFF4735 are separated by 86 nucleotides GFF4735 and GFF4736 overlap by 44 nucleotides
GFF4733 - Type III secretion transcriptional regulator HilC (= SirC), at 41,890 to 42,777
GFF4733
GFF4734 - Putative effector protein OrgC of SPI-1 type III secretion system, at 43,122 to 43,484
GFF4734
GFF4735 - OrgB protein, associated with InvC ATPase of type III secretion system, at 43,571 to 44,251
GFF4735
GFF4736 - Oxygen-regulated invasion protein OrgA, at 44,208 to 44,786
GFF4736
Position (kb) 43
44 Strain fitness (log2 ratio) -2
-1
0
1
2 at 42.284 kb on + strand, within GFF4733 at 43.026 kb on + strand at 43.070 kb on + strand at 43.081 kb on - strand at 43.082 kb on + strand at 43.085 kb on - strand at 43.085 kb on - strand at 43.097 kb on - strand at 43.365 kb on - strand, within GFF4734 at 43.476 kb on - strand at 43.546 kb on + strand at 43.547 kb on - strand at 43.616 kb on - strand at 43.682 kb on - strand, within GFF4735 at 43.754 kb on - strand, within GFF4735 at 43.778 kb on - strand, within GFF4735 at 43.784 kb on - strand, within GFF4735 at 44.014 kb on + strand, within GFF4735 at 44.122 kb on + strand, within GFF4735 at 44.188 kb on + strand at 44.251 kb on + strand at 44.252 kb on - strand at 44.426 kb on + strand, within GFF4736 at 44.440 kb on - strand, within GFF4736
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 42,284 + GFF4733 0.44 -1.0 43,026 + -0.6 43,070 + +0.5 43,081 - -0.1 43,082 + +2.2 43,085 - +0.5 43,085 - +0.4 43,097 - -0.3 43,365 - GFF4734 0.67 -0.2 43,476 - -1.9 43,546 + +0.4 43,547 - -0.4 43,616 - +0.4 43,682 - GFF4735 0.16 +1.4 43,754 - GFF4735 0.27 -0.3 43,778 - GFF4735 0.30 +1.1 43,784 - GFF4735 0.31 -0.1 44,014 + GFF4735 0.65 -0.2 44,122 + GFF4735 0.81 -0.5 44,188 + +0.5 44,251 + +0.9 44,252 - -1.1 44,426 + GFF4736 0.38 +0.0 44,440 - GFF4736 0.40 +0.2
Or see this region's nucleotide sequence