Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4659 and GFF4660 are separated by 43 nucleotides GFF4660 and GFF4661 are separated by 46 nucleotides GFF4661 and GFF4662 are separated by 15 nucleotides GFF4662 and GFF4663 are separated by 35 nucleotides GFF4663 and GFF4664 are separated by 35 nucleotides GFF4664 and GFF4665 are separated by 35 nucleotides GFF4665 and GFF4666 are separated by 35 nucleotides GFF4666 and GFF4667 are separated by 23 nucleotides GFF4667 and GFF4668 are separated by 8 nucleotides GFF4668 and GFF4582 are separated by 222 nucleotides
GFF4659 - tRNA-Gln-CTG, at 368,222 to 368,296
GFF4659
GFF4660 - tRNA-Gln-CTG, at 368,340 to 368,414
GFF4660
GFF4661 - tRNA-Met-CAT, at 368,461 to 368,537
GFF4661
GFF4662 - tRNA-Gln-TTG, at 368,553 to 368,627
GFF4662
GFF4663 - tRNA-Gln-TTG, at 368,663 to 368,737
GFF4663
GFF4664 - tRNA-Gln-TTG, at 368,773 to 368,847
GFF4664
GFF4665 - tRNA-Gln-TTG, at 368,883 to 368,957
GFF4665
GFF4666 - tRNA-Gln-TTG, at 368,993 to 369,067
GFF4666
GFF4667 - tRNA-Leu-TAG, at 369,091 to 369,175
GFF4667
GFF4668 - tRNA-Met-CAT, at 369,184 to 369,260
GFF4668
GFF4582 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 369,483 to 371,147
GFF4582
Position (kb)
369
370 Strain fitness (log2 ratio)
-1
0
1
2 at 368.132 kb on + strand at 368.174 kb on + strand at 368.460 kb on - strand at 369.661 kb on + strand, within GFF4582 at 369.661 kb on + strand, within GFF4582 at 369.662 kb on - strand, within GFF4582 at 369.662 kb on - strand, within GFF4582 at 369.688 kb on + strand, within GFF4582 at 369.777 kb on + strand, within GFF4582 at 369.830 kb on - strand, within GFF4582 at 369.997 kb on + strand, within GFF4582 at 369.997 kb on + strand, within GFF4582 at 369.998 kb on - strand, within GFF4582 at 370.001 kb on + strand, within GFF4582 at 370.001 kb on + strand, within GFF4582 at 370.001 kb on + strand, within GFF4582 at 370.002 kb on - strand, within GFF4582 at 370.002 kb on - strand, within GFF4582 at 370.002 kb on - strand, within GFF4582
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 368,132 + +0.7 368,174 + -0.0 368,460 - -0.5 369,661 + GFF4582 0.11 +0.7 369,661 + GFF4582 0.11 -0.4 369,662 - GFF4582 0.11 -0.1 369,662 - GFF4582 0.11 -0.3 369,688 + GFF4582 0.12 -0.3 369,777 + GFF4582 0.18 -1.3 369,830 - GFF4582 0.21 +2.3 369,997 + GFF4582 0.31 -0.4 369,997 + GFF4582 0.31 +1.0 369,998 - GFF4582 0.31 -0.1 370,001 + GFF4582 0.31 -0.1 370,001 + GFF4582 0.31 +0.8 370,001 + GFF4582 0.31 -0.8 370,002 - GFF4582 0.31 +0.2 370,002 - GFF4582 0.31 -0.6 370,002 - GFF4582 0.31 -1.6
Or see this region's nucleotide sequence