Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4649 and GFF4650 are separated by 291 nucleotides GFF4650 and GFF4651 overlap by 4 nucleotides GFF4651 and GFF4652 are separated by 3 nucleotides GFF4652 and GFF4653 are separated by 64 nucleotides
GFF4649 - Cyd operon protein YbgE, at 443,061 to 443,342
GFF4649
GFF4650 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 443,634 to 443,975
GFF4650
GFF4651 - MotA/TolQ/ExbB proton channel family protein, at 443,972 to 444,664
GFF4651
GFF4652 - Tol biopolymer transport system, TolR protein, at 444,668 to 445,096
GFF4652
GFF4653 - TolA protein, at 445,161 to 446,519
GFF4653
Position (kb) 443
444
445 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 443.004 kb on + strand at 443.004 kb on + strand at 443.004 kb on + strand at 443.004 kb on + strand at 443.012 kb on + strand at 443.056 kb on - strand at 443.056 kb on - strand at 443.068 kb on - strand at 443.074 kb on - strand at 443.141 kb on + strand, within GFF4649 at 443.141 kb on + strand, within GFF4649 at 443.141 kb on + strand, within GFF4649 at 443.141 kb on + strand, within GFF4649 at 443.142 kb on - strand, within GFF4649 at 443.142 kb on - strand, within GFF4649 at 443.142 kb on - strand, within GFF4649 at 443.142 kb on - strand, within GFF4649 at 443.204 kb on - strand, within GFF4649 at 443.441 kb on + strand at 443.444 kb on + strand at 443.445 kb on - strand at 443.445 kb on - strand at 443.445 kb on - strand at 443.463 kb on + strand at 443.487 kb on + strand at 443.487 kb on + strand at 443.496 kb on + strand at 443.508 kb on + strand at 443.576 kb on + strand at 443.580 kb on + strand at 443.581 kb on - strand at 443.652 kb on + strand at 443.769 kb on + strand, within GFF4650 at 443.793 kb on - strand, within GFF4650 at 443.881 kb on + strand, within GFF4650 at 443.945 kb on + strand at 444.376 kb on + strand, within GFF4651 at 444.376 kb on + strand, within GFF4651 at 444.376 kb on + strand, within GFF4651 at 444.409 kb on - strand, within GFF4651 at 444.662 kb on + strand at 444.703 kb on + strand at 444.719 kb on - strand, within GFF4652 at 444.739 kb on - strand, within GFF4652 at 444.764 kb on - strand, within GFF4652 at 444.841 kb on + strand, within GFF4652 at 444.862 kb on + strand, within GFF4652 at 444.888 kb on - strand, within GFF4652 at 444.951 kb on + strand, within GFF4652 at 445.234 kb on + strand at 445.235 kb on - strand at 445.271 kb on + strand at 445.350 kb on + strand, within GFF4653
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 443,004 + +0.2 443,004 + -1.3 443,004 + -2.9 443,004 + -1.0 443,012 + -1.9 443,056 - +1.2 443,056 - -0.1 443,068 - +2.2 443,074 - +0.2 443,141 + GFF4649 0.28 -0.3 443,141 + GFF4649 0.28 +0.2 443,141 + GFF4649 0.28 +0.2 443,141 + GFF4649 0.28 +0.0 443,142 - GFF4649 0.29 +0.0 443,142 - GFF4649 0.29 -0.2 443,142 - GFF4649 0.29 -0.0 443,142 - GFF4649 0.29 -0.8 443,204 - GFF4649 0.51 +0.4 443,441 + -0.5 443,444 + +0.1 443,445 - +2.6 443,445 - +2.6 443,445 - +2.1 443,463 + -0.5 443,487 + -1.2 443,487 + -0.5 443,496 + +1.1 443,508 + +0.2 443,576 + +0.4 443,580 + +1.3 443,581 - +3.0 443,652 + -0.2 443,769 + GFF4650 0.39 +0.2 443,793 - GFF4650 0.46 -0.6 443,881 + GFF4650 0.72 +0.5 443,945 + +0.6 444,376 + GFF4651 0.58 -0.2 444,376 + GFF4651 0.58 +3.0 444,376 + GFF4651 0.58 +3.0 444,409 - GFF4651 0.63 +4.2 444,662 + +1.6 444,703 + -1.9 444,719 - GFF4652 0.12 +4.4 444,739 - GFF4652 0.17 +3.2 444,764 - GFF4652 0.22 +2.4 444,841 + GFF4652 0.40 +1.2 444,862 + GFF4652 0.45 +1.0 444,888 - GFF4652 0.51 +0.7 444,951 + GFF4652 0.66 +0.9 445,234 + +2.9 445,235 - +2.2 445,271 + +1.4 445,350 + GFF4653 0.14 +2.6
Or see this region's nucleotide sequence