Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF461 and GFF462 overlap by 32 nucleotides GFF462 and GFF463 overlap by 23 nucleotides GFF463 and GFF464 are separated by 156 nucleotides
GFF461 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.5.3), at 180,425 to 182,758
GFF461
GFF462 - Acetyltransferase (EC 2.3.1.-), at 182,727 to 183,167
GFF462
GFF463 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 183,145 to 183,726
GFF463
GFF464 - FIG00543870: hypothetical protein, at 183,883 to 184,587
GFF464
Position (kb) 183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 182.253 kb on - strand, within GFF461 at 182.253 kb on - strand, within GFF461 at 182.378 kb on - strand, within GFF461 at 182.381 kb on + strand, within GFF461 at 182.381 kb on + strand, within GFF461 at 182.382 kb on - strand, within GFF461 at 182.757 kb on - strand at 183.034 kb on - strand, within GFF462 at 183.037 kb on + strand, within GFF462 at 183.100 kb on + strand, within GFF462 at 183.101 kb on - strand, within GFF462 at 183.101 kb on - strand, within GFF462 at 183.131 kb on + strand at 183.131 kb on + strand at 183.132 kb on - strand at 183.202 kb on - strand at 183.202 kb on - strand at 183.231 kb on + strand, within GFF463 at 183.516 kb on + strand, within GFF463 at 183.517 kb on - strand, within GFF463 at 183.687 kb on + strand at 183.687 kb on + strand at 183.687 kb on + strand at 183.688 kb on - strand at 183.689 kb on + strand at 183.689 kb on + strand at 183.690 kb on - strand at 183.690 kb on - strand at 183.690 kb on - strand at 183.726 kb on + strand at 183.727 kb on - strand at 183.727 kb on - strand at 183.799 kb on + strand at 183.800 kb on - strand at 183.803 kb on + strand at 183.806 kb on + strand at 183.845 kb on - strand at 183.847 kb on + strand at 183.890 kb on + strand at 183.961 kb on - strand, within GFF464 at 183.967 kb on + strand, within GFF464 at 183.967 kb on + strand, within GFF464 at 183.985 kb on + strand, within GFF464 at 183.986 kb on - strand, within GFF464 at 184.025 kb on - strand, within GFF464 at 184.114 kb on - strand, within GFF464 at 184.220 kb on - strand, within GFF464 at 184.243 kb on + strand, within GFF464 at 184.243 kb on + strand, within GFF464 at 184.243 kb on + strand, within GFF464 at 184.243 kb on + strand, within GFF464 at 184.244 kb on - strand, within GFF464 at 184.252 kb on + strand, within GFF464 at 184.274 kb on + strand, within GFF464 at 184.372 kb on + strand, within GFF464 at 184.372 kb on + strand, within GFF464 at 184.372 kb on + strand, within GFF464 at 184.373 kb on - strand, within GFF464 at 184.375 kb on - strand, within GFF464 at 184.469 kb on + strand, within GFF464 at 184.484 kb on + strand, within GFF464 at 184.485 kb on - strand, within GFF464 at 184.485 kb on - strand, within GFF464 at 184.586 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 182,253 - GFF461 0.78 +1.6 182,253 - GFF461 0.78 +0.1 182,378 - GFF461 0.84 -0.8 182,381 + GFF461 0.84 +0.4 182,381 + GFF461 0.84 -0.0 182,382 - GFF461 0.84 -1.0 182,757 - -1.4 183,034 - GFF462 0.70 +0.2 183,037 + GFF462 0.70 +0.5 183,100 + GFF462 0.85 +0.3 183,101 - GFF462 0.85 -0.6 183,101 - GFF462 0.85 -1.5 183,131 + +1.1 183,131 + -0.4 183,132 - -0.2 183,202 - -1.1 183,202 - +0.6 183,231 + GFF463 0.15 -1.3 183,516 + GFF463 0.64 +0.0 183,517 - GFF463 0.64 -0.7 183,687 + +1.6 183,687 + +0.5 183,687 + +0.2 183,688 - -0.1 183,689 + -0.5 183,689 + +0.4 183,690 - +2.0 183,690 - +0.4 183,690 - -0.9 183,726 + -1.2 183,727 - +0.2 183,727 - -1.4 183,799 + -1.1 183,800 - -0.0 183,803 + -0.4 183,806 + -1.5 183,845 - +1.3 183,847 + +1.6 183,890 + -0.1 183,961 - GFF464 0.11 -0.1 183,967 + GFF464 0.12 +0.2 183,967 + GFF464 0.12 -2.9 183,985 + GFF464 0.14 -0.5 183,986 - GFF464 0.15 -0.1 184,025 - GFF464 0.20 -0.4 184,114 - GFF464 0.33 -0.5 184,220 - GFF464 0.48 +0.6 184,243 + GFF464 0.51 -1.5 184,243 + GFF464 0.51 -0.7 184,243 + GFF464 0.51 +0.9 184,243 + GFF464 0.51 +4.1 184,244 - GFF464 0.51 +0.9 184,252 + GFF464 0.52 -0.5 184,274 + GFF464 0.55 +0.2 184,372 + GFF464 0.69 +0.0 184,372 + GFF464 0.69 +0.8 184,372 + GFF464 0.69 -0.5 184,373 - GFF464 0.70 -0.5 184,375 - GFF464 0.70 +0.1 184,469 + GFF464 0.83 -0.3 184,484 + GFF464 0.85 -0.0 184,485 - GFF464 0.85 +1.0 184,485 - GFF464 0.85 +1.0 184,586 - -0.2
Or see this region's nucleotide sequence