Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4588 and GFF4589 are separated by 35 nucleotides GFF4589 and GFF4590 are separated by 296 nucleotides GFF4590 and GFF4591 are separated by 49 nucleotides GFF4591 and GFF4592 are separated by 48 nucleotides
GFF4588 - Glutaminyl-tRNA synthetase (EC 6.1.1.18), at 377,965 to 379,632
GFF4588
GFF4589 - hypothetical protein, at 379,668 to 379,781
GFF4589
GFF4590 - N-acetylglucosamine-regulated outer membrane porin, at 380,078 to 381,484
GFF4590
GFF4591 - Hypothetical lipoprotein ybfN precursor, at 381,534 to 381,866
GFF4591
GFF4592 - TcuC: integral membrane protein used to transport tricarballylate across the cell membrane, at 381,915 to 383,219
GFF4592
Position (kb) 380
381
382 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 379.609 kb on - strand at 379.930 kb on + strand at 380.072 kb on + strand at 380.072 kb on + strand at 380.073 kb on - strand at 380.101 kb on + strand at 380.104 kb on + strand at 380.213 kb on + strand at 380.272 kb on + strand, within GFF4590 at 380.501 kb on + strand, within GFF4590 at 380.588 kb on + strand, within GFF4590 at 380.588 kb on + strand, within GFF4590 at 380.735 kb on + strand, within GFF4590 at 380.813 kb on + strand, within GFF4590 at 380.844 kb on - strand, within GFF4590 at 380.932 kb on + strand, within GFF4590 at 380.937 kb on - strand, within GFF4590 at 380.937 kb on - strand, within GFF4590 at 381.045 kb on - strand, within GFF4590 at 381.058 kb on + strand, within GFF4590 at 381.071 kb on + strand, within GFF4590 at 381.164 kb on + strand, within GFF4590 at 381.165 kb on - strand, within GFF4590 at 381.213 kb on - strand, within GFF4590 at 381.266 kb on + strand, within GFF4590 at 381.266 kb on + strand, within GFF4590 at 381.302 kb on + strand, within GFF4590 at 381.321 kb on - strand, within GFF4590 at 381.395 kb on - strand at 381.534 kb on - strand at 381.631 kb on - strand, within GFF4591 at 381.674 kb on - strand, within GFF4591 at 381.762 kb on + strand, within GFF4591 at 381.762 kb on + strand, within GFF4591 at 381.854 kb on + strand at 381.940 kb on + strand at 381.991 kb on - strand at 381.991 kb on - strand at 382.133 kb on - strand, within GFF4592 at 382.133 kb on - strand, within GFF4592 at 382.133 kb on - strand, within GFF4592 at 382.133 kb on - strand, within GFF4592 at 382.177 kb on + strand, within GFF4592 at 382.178 kb on - strand, within GFF4592 at 382.289 kb on + strand, within GFF4592 at 382.290 kb on - strand, within GFF4592 at 382.456 kb on + strand, within GFF4592 at 382.456 kb on + strand, within GFF4592
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 379,609 - +4.2 379,930 + -0.0 380,072 + +3.0 380,072 + -0.4 380,073 - +1.2 380,101 + -1.8 380,104 + +0.5 380,213 + +0.8 380,272 + GFF4590 0.14 +1.7 380,501 + GFF4590 0.30 +0.3 380,588 + GFF4590 0.36 +0.6 380,588 + GFF4590 0.36 +0.8 380,735 + GFF4590 0.47 -0.9 380,813 + GFF4590 0.52 +0.6 380,844 - GFF4590 0.54 +0.3 380,932 + GFF4590 0.61 +2.3 380,937 - GFF4590 0.61 +1.5 380,937 - GFF4590 0.61 -1.0 381,045 - GFF4590 0.69 +0.6 381,058 + GFF4590 0.70 +0.7 381,071 + GFF4590 0.71 -0.8 381,164 + GFF4590 0.77 -0.2 381,165 - GFF4590 0.77 +0.9 381,213 - GFF4590 0.81 +0.7 381,266 + GFF4590 0.84 +0.3 381,266 + GFF4590 0.84 +1.5 381,302 + GFF4590 0.87 +0.6 381,321 - GFF4590 0.88 +2.5 381,395 - +0.4 381,534 - -1.0 381,631 - GFF4591 0.29 +0.5 381,674 - GFF4591 0.42 -0.3 381,762 + GFF4591 0.68 -0.9 381,762 + GFF4591 0.68 +0.5 381,854 + +0.4 381,940 + +0.2 381,991 - +1.7 381,991 - -1.0 382,133 - GFF4592 0.17 +1.9 382,133 - GFF4592 0.17 +1.3 382,133 - GFF4592 0.17 +0.7 382,133 - GFF4592 0.17 +1.0 382,177 + GFF4592 0.20 +0.6 382,178 - GFF4592 0.20 -0.2 382,289 + GFF4592 0.29 -0.2 382,290 - GFF4592 0.29 +0.1 382,456 + GFF4592 0.41 +0.5 382,456 + GFF4592 0.41 -0.6
Or see this region's nucleotide sequence