Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4487 and GFF4488 are separated by 11 nucleotides GFF4488 and GFF4489 are separated by 45 nucleotides GFF4489 and GFF4490 overlap by 4 nucleotides GFF4490 and GFF4491 are separated by 48 nucleotides
GFF4487 - FIG00638355: hypothetical protein, at 269,892 to 271,010
GFF4487
GFF4488 - '4'-phosphopantetheinyl transferase (EC 2.7.8.-) [enterobactin] siderophore' transl_table=11, at 271,022 to 271,726
GFF4488
GFF4489 - TonB-dependent receptor; Outer membrane receptor for ferric enterobactin and colicins B, D, at 271,772 to 274,027
GFF4489
GFF4490 - hypothetical protein, at 274,024 to 274,167
GFF4490
GFF4491 - Enterobactin esterase, at 274,216 to 275,430
GFF4491
Position (kb)
271
272
273
274
275 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 270.777 kb on - strand, within GFF4487 at 270.840 kb on - strand, within GFF4487 at 270.851 kb on + strand, within GFF4487 at 270.852 kb on - strand, within GFF4487 at 270.922 kb on - strand at 270.928 kb on + strand at 270.974 kb on + strand at 270.975 kb on - strand at 270.975 kb on - strand at 271.043 kb on + strand at 271.043 kb on + strand at 271.044 kb on - strand at 271.044 kb on - strand at 271.045 kb on + strand at 271.099 kb on + strand, within GFF4488 at 271.102 kb on + strand, within GFF4488 at 271.103 kb on - strand, within GFF4488 at 271.108 kb on + strand, within GFF4488 at 271.141 kb on + strand, within GFF4488 at 271.142 kb on - strand, within GFF4488 at 271.182 kb on - strand, within GFF4488 at 271.261 kb on + strand, within GFF4488 at 271.355 kb on - strand, within GFF4488 at 271.544 kb on + strand, within GFF4488 at 271.591 kb on - strand, within GFF4488 at 271.629 kb on - strand, within GFF4488 at 271.786 kb on + strand at 271.786 kb on + strand at 271.787 kb on - strand at 271.796 kb on - strand at 271.797 kb on + strand at 271.797 kb on + strand at 271.805 kb on - strand at 271.805 kb on - strand at 271.805 kb on - strand at 271.805 kb on - strand at 271.805 kb on - strand at 271.821 kb on + strand at 271.821 kb on + strand at 271.822 kb on - strand at 271.822 kb on - strand at 271.846 kb on + strand at 271.848 kb on + strand at 271.849 kb on - strand at 271.918 kb on + strand at 271.981 kb on + strand at 271.982 kb on - strand at 271.987 kb on - strand at 272.040 kb on + strand, within GFF4489 at 272.064 kb on + strand, within GFF4489 at 272.103 kb on - strand, within GFF4489 at 272.189 kb on - strand, within GFF4489 at 272.190 kb on + strand, within GFF4489 at 272.190 kb on + strand, within GFF4489 at 272.191 kb on - strand, within GFF4489 at 272.191 kb on - strand, within GFF4489 at 272.191 kb on - strand, within GFF4489 at 272.191 kb on - strand, within GFF4489 at 272.289 kb on + strand, within GFF4489 at 272.289 kb on + strand, within GFF4489 at 272.290 kb on - strand, within GFF4489 at 272.315 kb on - strand, within GFF4489 at 272.348 kb on - strand, within GFF4489 at 272.348 kb on - strand, within GFF4489 at 272.422 kb on + strand, within GFF4489 at 272.432 kb on - strand, within GFF4489 at 272.467 kb on - strand, within GFF4489 at 272.547 kb on + strand, within GFF4489 at 272.653 kb on + strand, within GFF4489 at 272.654 kb on - strand, within GFF4489 at 272.768 kb on - strand, within GFF4489 at 272.830 kb on + strand, within GFF4489 at 272.910 kb on - strand, within GFF4489 at 273.017 kb on - strand, within GFF4489 at 273.025 kb on - strand, within GFF4489 at 273.038 kb on - strand, within GFF4489 at 273.038 kb on - strand, within GFF4489 at 273.124 kb on + strand, within GFF4489 at 273.254 kb on - strand, within GFF4489 at 273.338 kb on - strand, within GFF4489 at 273.520 kb on + strand, within GFF4489 at 273.521 kb on - strand, within GFF4489 at 273.785 kb on + strand, within GFF4489 at 273.987 kb on - strand at 273.999 kb on - strand at 274.030 kb on + strand at 274.031 kb on - strand at 274.031 kb on - strand at 274.372 kb on + strand, within GFF4491 at 274.372 kb on + strand, within GFF4491 at 274.372 kb on + strand, within GFF4491 at 274.490 kb on - strand, within GFF4491 at 274.497 kb on + strand, within GFF4491 at 274.586 kb on + strand, within GFF4491 at 274.587 kb on - strand, within GFF4491 at 274.773 kb on - strand, within GFF4491 at 274.822 kb on - strand, within GFF4491 at 274.938 kb on + strand, within GFF4491
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 270,777 - GFF4487 0.79 -1.5 270,840 - GFF4487 0.85 +2.2 270,851 + GFF4487 0.86 -0.7 270,852 - GFF4487 0.86 -1.6 270,922 - +0.9 270,928 + -0.0 270,974 + +0.4 270,975 - -0.1 270,975 - +0.8 271,043 + +0.7 271,043 + -2.0 271,044 - +0.6 271,044 - +0.0 271,045 + +2.2 271,099 + GFF4488 0.11 +0.1 271,102 + GFF4488 0.11 +0.1 271,103 - GFF4488 0.11 -0.5 271,108 + GFF4488 0.12 +0.9 271,141 + GFF4488 0.17 +0.2 271,142 - GFF4488 0.17 -0.4 271,182 - GFF4488 0.23 +2.4 271,261 + GFF4488 0.34 +0.5 271,355 - GFF4488 0.47 +1.0 271,544 + GFF4488 0.74 +1.1 271,591 - GFF4488 0.81 -0.3 271,629 - GFF4488 0.86 +0.4 271,786 + +0.5 271,786 + -1.1 271,787 - +0.2 271,796 - -1.0 271,797 + -0.9 271,797 + +0.8 271,805 - -0.8 271,805 - -0.1 271,805 - -2.2 271,805 - -0.4 271,805 - -1.6 271,821 + -1.7 271,821 + +0.6 271,822 - -0.2 271,822 - -0.7 271,846 + +1.6 271,848 + -1.6 271,849 - -1.2 271,918 + -0.1 271,981 + -1.0 271,982 - -1.7 271,987 - -1.5 272,040 + GFF4489 0.12 +0.1 272,064 + GFF4489 0.13 +0.3 272,103 - GFF4489 0.15 -1.0 272,189 - GFF4489 0.18 +1.4 272,190 + GFF4489 0.19 +0.3 272,190 + GFF4489 0.19 -0.2 272,191 - GFF4489 0.19 -0.5 272,191 - GFF4489 0.19 -0.7 272,191 - GFF4489 0.19 +0.5 272,191 - GFF4489 0.19 -2.8 272,289 + GFF4489 0.23 +0.2 272,289 + GFF4489 0.23 +0.2 272,290 - GFF4489 0.23 -0.6 272,315 - GFF4489 0.24 -0.1 272,348 - GFF4489 0.26 -0.4 272,348 - GFF4489 0.26 +0.3 272,422 + GFF4489 0.29 -0.5 272,432 - GFF4489 0.29 -0.4 272,467 - GFF4489 0.31 -0.1 272,547 + GFF4489 0.34 -0.7 272,653 + GFF4489 0.39 +0.7 272,654 - GFF4489 0.39 +0.0 272,768 - GFF4489 0.44 +1.9 272,830 + GFF4489 0.47 -2.3 272,910 - GFF4489 0.50 +0.6 273,017 - GFF4489 0.55 +0.4 273,025 - GFF4489 0.56 -1.3 273,038 - GFF4489 0.56 -1.4 273,038 - GFF4489 0.56 -2.1 273,124 + GFF4489 0.60 -1.6 273,254 - GFF4489 0.66 +0.1 273,338 - GFF4489 0.69 -2.2 273,520 + GFF4489 0.77 +1.4 273,521 - GFF4489 0.78 +0.7 273,785 + GFF4489 0.89 -1.6 273,987 - -1.5 273,999 - +0.3 274,030 + -0.1 274,031 - +0.1 274,031 - -1.5 274,372 + GFF4491 0.13 -2.5 274,372 + GFF4491 0.13 -1.3 274,372 + GFF4491 0.13 -0.8 274,490 - GFF4491 0.23 -2.8 274,497 + GFF4491 0.23 -3.7 274,586 + GFF4491 0.30 -0.7 274,587 - GFF4491 0.31 -3.4 274,773 - GFF4491 0.46 -3.9 274,822 - GFF4491 0.50 +0.3 274,938 + GFF4491 0.59 -2.3
Or see this region's nucleotide sequence